GLVR1抗原；長(zhǎng)臂猿白血病病毒受體1(GLVR1)重組蛋白多種屬 是應(yīng)用了重組DNA或重組RNA的技術(shù)從而獲得的蛋白質(zhì)。體外重組蛋白的生產(chǎn)主要包括四大系統(tǒng):原核蛋白表達(dá)，哺乳動(dòng)物細(xì)胞蛋白表達(dá)，酵母蛋白表達(dá)及昆蟲(chóng)細(xì)胞蛋白表達(dá)。生產(chǎn)的蛋白在活性和應(yīng)用方法方面均有所不同。根據(jù)自身的下游運(yùn)用選擇合適的蛋白表達(dá)系統(tǒng)，提高表達(dá)成功率。

Recombinant Gibbon Ape Leukemia Virus Receptor 1 (GLVR1)

長(zhǎng)臂猿白血病病毒受體1(GLVR1)重組蛋白

[ PROPERTIES ]

Article Number:XY1066Hu01

Specifications and prices: 50ug 1800.00 100ug 2880.00

Residues:Cys251~Glu510

Tags:Two N-terminal Tags, His-tag and T7-tag

Accession:Q8WUM9

Host:E. coli

Subcellular Location: Membrane; Multi-passMembrane Protein.

Purity:>90%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation:Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point:5.4

Predicted Molecular Mass:32.8kDa

Accurate Molecular Mass:37kDa as determined by SDS-PAGE reducingconditions.

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH₂O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8°Cfor one month.

             Aliquot and store at -80°Cfor 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE VGPATVPLQAVVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG AVQLPNGNLV QFSQAVSNQINSSGHYQYHT VHKDSGLYKE LLHKLHLAKV GDCMGDSGDK PLRRNNSYTS YTMAICGMPLDSFRAKEGEQ KGEEMEKLTW PNADSKKRIR MDSYTSYCNA VSDLHSASEI DMSVKAEMGLGDRKGSNGSL EEWYDQDKPE

[  IDENTIFICATION  ]

特別提示:本公司的所有產(chǎn)品僅可用于科研實(shí)驗(yàn),嚴(yán)禁用于臨床醫(yī)療及其他非科研用途!以實(shí)際收貨產(chǎn)品說(shuō)明書為準(zhǔn),網(wǎng)站說(shuō)明書僅供參考！

關(guān)鍵詞：SLC20A1    PIT1  Glvr-1    PiT-1  Leukemia  virus  receptor  1  homolog    Phosphate  transporter  1  

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