【英語名稱】:TNFRSF18 (Human) ELISA Kit
【中文名稱】:TNFRSF18 (人源) ELISA試劑盒
【產(chǎn)品類型】:免疫學試劑盒【供應商】:Abnova
【產(chǎn)品貨號】:KA1719
【產(chǎn)品規(guī)格】:1 Kit
【背景資料】:TNFRSF18 (人源) ELISA試劑盒產(chǎn)品為Abnova旗下提供的高品質(zhì)試劑盒產(chǎn)品。作為多家國際知名試劑盒品牌的中國區(qū)域代理,艾美捷能為您提供更多TNFRSF18 (人源) ELISA試劑盒產(chǎn)品或相關(guān)產(chǎn)品的解決方案。
【保存建議】:請在接收到TNFRSF18 (人源) ELISA試劑盒產(chǎn)品后,按照產(chǎn)品操作說明書對試劑盒內(nèi)不同組份進行適當?shù)谋4妗?/p>
【產(chǎn)品說明】:點擊:TNFRSF18 (人源) ELISA試劑盒查看更詳細產(chǎn)品說明,更多應用、儲存、價格、貨期等信息請致電400-6800-868垂詢Abnova中國區(qū)域授權(quán)總代理艾美捷科技有限公司。
作為全世界最大的單克隆抗體和蛋白生產(chǎn)商,Abnova的金牌品質(zhì)使其成為全球最主要抗體品牌的供應商,您可以在各個主要抗體品牌,如Abcam、Sigma、Novus、AbD等知名品牌的目錄上都能發(fā)現(xiàn)Abnova的抗體。作為Abnova的中國區(qū)域代理,艾美捷科技有限公司憑借豐富的專業(yè)經(jīng)驗,將一如既往的為中國區(qū)域客戶提供Abnova優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務,包括各種抗體、重組蛋白、抗體對、ELISA試劑盒、原位雜交熒光探針、蛋白定量以及可用于蛋白翻譯研究、診斷和制藥公司應用的基礎(chǔ)型體外診斷設(shè)備等。
T4GCW D1600E-S0 F1600E-S0 R830 R730 DELL服務器電源批發(fā)及專業(yè)電源維修批發(fā)TEL:13910098771
TPJ2X 5NF18 D750E-S7 Y3HJ8 TFR9V M12Y2 W12Y2 02RN7 E1100D-S0 T4GCW 95HR5 D1600E-S0 F1600E-S0 R830 T630 R930 T430 R530 R630 R730 R730XD 495W 750W 1100W 1600W DELL Poweredge 13G服務器13G服務器電源批發(fā)及專業(yè)電源維修D(zhuǎn)ELL服務器DELL磁盤陣列柜DELL刀片DELL工作站DELL交換機等整機及新老配件批發(fā)TEL:13910098771
TPJ2X 5NF18 D750E-S7 Y3HJ8 TFR9V M12Y2 W12Y2 02RN7 E1100D-S0 T4GCW 95HR5 D1600E-S0 F1600E-S0 R830 T630 R930 T430 R530 R630 R730 R730XD 495W 750W 1100W 1600W DELL Poweredge 13G服務器13G服務器電源批發(fā)及專業(yè)電源維修D(zhuǎn)ELL服務器DELL磁盤陣列柜DELL刀片DELL工作站DELL交換機等整機及新老配件批發(fā)TEL:13910098771
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樣品收集、處理及保存方法
樣品采集:收集標本前必須清楚要檢測的成份是否足夠穩(wěn)定。對收集后當天進行檢測的標本,儲存在4℃?zhèn)溆,如有特殊原因需要周期收集標本?/span>將標本及時分裝后放在-20℃或-70℃條件下保存。避免反復凍融。標本2-8℃可保存48小時,-20℃可保存1個月。-70度可保存6個月。 部分激素類標本需添加抑肽酶。人腫瘤壞死因子受體超家族成員18(TNFRSF18)ELISA試劑盒操作步驟:1. 使用前,將所有試劑充分混勻。不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差。2. 根據(jù)待測樣品數(shù)量加上標準品的數(shù)量決定所需的板條數(shù)。每個標準品和空白孔建議做復孔。每個樣品根據(jù)自己的數(shù)量來定,能使用復孔的盡量做復孔。3. 加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內(nèi)。立即加入50ul的生物素標記的抗體。蓋上膜板,輕輕振蕩混勻,37℃溫育1小時。4. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復此操作3次。如果用洗板機洗滌,洗滌次數(shù)增加一次。5. 每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘。6. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復此操作3次。如果用洗板機洗滌,洗滌次數(shù)增加一次。7. 每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘。避免光照。8. 取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結(jié)果。9. 在450nm波長處測定各孔的OD值。人腫瘤壞死因子受體超家族成員18(TNFRSF18)ELISA試劑盒結(jié)果判斷與分析
1、儀器值:于波長450nm的酶標儀上讀取各孔的OD值
2、以吸光度OD值為縱坐標(Y),相應的待測物質(zhì)標準品濃度為橫坐標(X),在坐標紙上做得相應的曲線,樣品的待測物質(zhì)含量可根據(jù)其OD值由標準曲線換算出相應的濃度。
注意事項
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歡迎垂詢超凡機械盡在HF1800型摩托車破碎機F
鞏義市宏富機械制造有限公司研發(fā)部根據(jù)廢舊金屬回收市場的迫切需要,組織力量并結(jié)合國內(nèi)外客戶的具體應用實際研制生產(chǎn)出新型、高效、節(jié)能、環(huán)保的新一代金屬破碎機,具有操作簡單方便、結(jié)構(gòu)緊促、產(chǎn)出量大等顯著優(yōu)點。該金屬破碎機主要運用于大型廢品回收站、廢金屬回收公司等,可以將廢舊自行車、摩托車、廢舊電線、易拉罐、啤酒瓶蓋、鐵盒、油漆桶等金屬破碎成理想的顆粒,從而大大方便運輸、加工和減少運輸成本。用戶可根據(jù)所要加工原料的種類和對成品物料的要求(破碎顆粒大。┻x購不同的機型和配置。宏富金屬破碎機憑借技術(shù)先進、質(zhì)量可靠、價格公平的優(yōu)勢早已遠銷南非、澳大利亞、俄羅斯等地并得到國內(nèi)外客戶的一致好評并。
HF1800型摩托車破碎機引爆耀眼商機
熱烈慶祝HF1800型摩托車破碎機成功銷往馬來西亞,這種破碎機是目前市場上比較大型的金屬破碎設(shè)備,具有扭力大,產(chǎn)量高,省動力,耐磨和等優(yōu)點!HF1800摩托車破碎機的工作原理:是利用錘子擊打的基本原理,上料口帶有過濾式傳送帶,在高速大扭矩電機的驅(qū)動下,主機轉(zhuǎn)子上的錘頭輪流擊打進入容腔內(nèi)的待破碎物,通過襯板與錘頭之間形成的空間,將待破碎物撕裂成合乎規(guī)格的破碎物。
鞏義市宏富機械制造有限公司旨在為您提供更好的產(chǎn)品及最為經(jīng)濟合理的解決方案,我們的技術(shù)專家隨時為您提供技術(shù)幫助,并希望與您建立長期的、追求更高價值的合作關(guān)系!