亚洲视频福利,日本精品一区二区三区在线,日韩欧美在线视频一区二区,国产高清自产拍av在线,99久久99久久精品国产片果冻,国产视频手机在线,成人禁在线观看午夜亚洲

人白介素10(IL-10)Elisa快速檢測試劑盒

特 異 性：本elisa試劑盒可同時檢測天然或重組的，且與其他相關蛋白無交叉反應。

檢測種屬：人、大小鼠、兔、羊、猴、豬、豚鼠ELISA檢測試劑盒等種屬。

樣本范圍：血清、血漿、細胞上清液、尿液、體液、灌洗液、腦脊髓、心房水、胸房水、組織等。

儲存方式人白介素10(IL-10)Elisa快速檢測試劑盒

存儲條件：4℃保存

有 效 期：6個月

產(chǎn)品規(guī)格：96T/48T

適用范圍：此試劑盒僅用于科研實驗，嚴禁用于臨床。

產(chǎn)品特點人白介素10(IL-10)Elisa快速檢測試劑盒本公司對所有售出的產(chǎn)品保證質(zhì)量，并提供良好的售后服務，凡購買本公司任意款Elisa試劑盒，均可享受免費代測服務，并可全程提供技術指導。

用特異性抗體包被于固相載體，經(jīng)洗滌后加入含有抗原之待測樣品，如待檢樣品中有相應抗原存在，即可與包被于固相載體上的特異性抗體結合，經(jīng)保溫孵育洗滌后，即可加入酶標記特異性抗體，再經(jīng)孵育洗滌后，加底物顯色進行測定，底物降解的量即為欲測抗原的量。這種方法欲測的抗原必須有兩個可以與抗體結合的部位，因為其一端要包被于固相載體上的抗體作用，而另一端則要與酶標記特異性抗體作用。因此，不能用于分子量小于5000的半抗原之類的抗原測定。

可以：（1）免疫酶染色各種細胞內(nèi)成份的定位。

     （2）研究抗酶抗體的合成。

     （3）顯現(xiàn)微量的免疫沉淀反應。

     （4）定量檢測體液中抗原或抗體成份。

血漿樣本直接測定前處理方法：

（1）加酶抑制劑：準確采全血若干毫升，注入預冷的加有抗凝劑的塑料指形管中，迅速低溫離心，取血漿低溫保存待測。抑肽酶有液體的與固體的。固體的抑肽酶可用生理鹽水溶解。

（2）酸化處理：每毫升血漿加1mol/l HCl 0.2ml，低溫保存待測。以上兩種方法，任選一種即可。

血漿樣本的間接測定 血標本經(jīng)提取后，可去除蛋白質(zhì)等干擾因素，并使微量的生物活性肽濃縮，但較費時，成本也高。常用的提取方法有：

（1）柱層析提取法：有多種層析柱可供提取不同的神經(jīng)肽，其中較為方便的是用Sep-pak C18層析柱提取。主要步驟：

①柱的預處理：該柱有兩頭，長頭連接注射器，可注入溶液與樣品，短頭為排出口。預處理時注入乙腈10ml。蒸餾水20ml，使柱中的生物膠活化。

②注入樣品（如血漿、腦脊液、腹水、尿等）：樣品中的水份流出，生物活性肽、蛋白及雜質(zhì)等吸附在生物膠上。血漿上柱前，如先去除蛋白，可增加柱子的使用時間。

③淋洗：用4%HAC溶液100ml注入柱中，以洗去一些雜質(zhì)。

④洗脫：用7ml酸性乙腈作為洗脫劑，注入柱中，把生物活性肽洗脫下來，收集在塑料指形管中。

⑤清洗：用乙腈、蒸餾水清洗柱子，以備后用。

⑥將洗脫液吹干，低溫保存待測。測定時可加入一定量的緩沖液溶解。

人白介素10(IL-10)Elisa快速檢測試劑盒

YS01050B人細胞凋亡抑制因子(IAP)elisa試劑盒

YS01051B人集落刺激因子(CSF)Elisa試劑盒

YS01052B人γ干擾素誘導單核細胞因子(MIGF)Elisa試劑盒

YS01053B人干擾素誘導T細胞趨化因子(ITAC)Elisa試劑盒

YS01054B人CD14分子(CDl4)Elisa試劑盒

YS01055B人凋亡誘導因子(AIF)Elisa試劑盒

YS01056B人白細胞共同抗原(LCA)Elisa試劑盒

YS01057B人CD4分子(CD4)Elisa試劑盒

YS01058B人P鈣黏蛋白/胎盤鈣黏蛋白(P-cad)Elisa試劑盒

YS01059B人角化細胞生長因子(KGF)Elisa試劑盒

YS01060B人血小板衍生生長因子BB(PDGF-BB)Elisa試劑盒

YS01061B人CXC趨化因子配體16(CXCL16)Elisa試劑盒

YS01062B人CXC趨化因子受體3(CXCR3)Elisa試劑盒

YS01063B人γ干擾素誘導蛋白16(IFI16)Elisa試劑盒

YS01064B人基質(zhì)細胞衍生因子1a(SDF-1a)Elisa試劑盒

YS01065B人淋巴細胞趨化因子(Lptn)Elisa試劑盒

YS01066B人α干擾素(IFN-α)Elisa試劑盒

YS01067B人可溶性CD86(sCD86)Elisa試劑盒

YS01068B人白介素27(IL-27)Elisa試劑盒

YS01069B人白介素23(IL-23)Elisa試劑盒

YS01070B人巨噬細胞移動抑制因子(MIF)Elisa試劑盒

YS01071B人組織因子途徑抑制物(TFPI)Elisa試劑盒

YS01072B人干擾素誘導蛋白10(IP-10)Elisa試劑盒

YS01073B人白介素1(IL-1)Elisa試劑盒

YS01074B人白介素17(IL-17)Elisa試劑盒

YS01075B人白介素1β(IL-1β)Elisa試劑盒

YS01076B人表皮生長因子(EGF)Elisa試劑盒

YS01077B人堿性成纖維細胞生長因子(bFGF)Elisa試劑盒

YS01078B人巨噬細胞炎性蛋白5(MIP-5)Elisa試劑盒

YS01079B人可溶性E選擇素(sE-selectin)Elisa試劑盒

YS01080B人可溶性細胞間粘附分子1(sICAM-1)Elisa試劑盒

YS01081B人細胞間粘附分子2(ICAM-2)Elisa試劑盒

YS01082B人細胞間粘附分子3(ICAM-3)Elisa試劑盒

YS01083B人結締組織生長因子(CTGF)Elisa試劑盒

YS01084B人白介素18(IL-18)Elisa試劑盒

YS01085B人粘膜相關上皮趨化因子(MEC)Elisa試劑盒

YS01086B人B細胞活化因子受體(BAFF-R)Elisa試劑盒

YS01087B人血管內(nèi)皮細胞生長因子受體3(VEGFR-3)Elisa試劑盒

YS01088B人血管內(nèi)皮細胞生長因子受體1(VEGFR-1)Elisa試劑盒

YS01089B人血管內(nèi)皮細胞生長因子D(VEGF-D)Elisa試劑盒

YS01090B人血管內(nèi)皮細胞生長因子C(VEGF-C)Elisa試劑盒

YS01091B人血管內(nèi)皮細胞生長因子A(VEGF-A)Elisa試劑盒

YS01092B人血管內(nèi)皮細胞生長因子B(VEGF-B)Elisa試劑盒

YS01093B人血管內(nèi)皮細胞生長因子(VEGF)Elisa試劑盒

YS01094B人血管內(nèi)皮細胞粘附分子1(VCAM-1)Elisa試劑盒

YS01095B人可溶性腫瘤壞死因子相關凋亡誘導配體(sTRAIL)Elisa試劑盒

YS01096B人腫瘤壞死因子相關凋亡誘導配體4(TRAIL-R4)Elisa試劑盒

YS01097B人腫瘤壞死因子相關凋亡誘導配體3(TRAIL-R3)Elisa試劑盒

YS01098B人腫瘤壞死因子相關凋亡誘導配體1(TRAIL-R1)Elisa試劑盒

YS01099B人腫瘤壞死因子β(TNF-β)Elisa試劑盒

YS01100B人腫瘤壞死因子α(TNF-α)Elisa試劑盒

YS01101B人腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)Elisa試劑盒

YS01102B人腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)Elisa試劑盒

YS01103B人轉(zhuǎn)化生長因子β1(TGF-β1)Elisa試劑盒

YS01104B人轉(zhuǎn)化生長因子α(TGF-α)Elisa試劑盒

YS01105B人基質(zhì)細胞衍生因子1β(SDF-1β)Elisa試劑盒

YS01106B人干細胞因子受體(SCFR)Elisa試劑盒

YS01107B人干細胞因子/肥大細胞生長因子(SCF/MGF)Elisa試劑盒

YS01108B人可溶性CD40配體(sCD40L)Elisa試劑盒

YS01109B人可溶性CD30配體(sCD30L)Elisa試劑盒

操作流程我公司現(xiàn)貨供應，全國包郵，提供免費代測服務，全程提供技術支持，質(zhì)量保證，歡迎新老客戶電話咨詢我公司免費索取該試劑盒或相關Elisa試劑盒的詳細說明書及價格信息?？蛻艉迷u歡迎新老客戶咨詢訂購：人白介素10(IL-10)Elisa快速檢測試劑盒

規(guī)格：96T/48T

貨期：2~3天

保存溫度：2-8℃

用途：用于測定兔血清、血漿、組織及相關液體樣本中的含量或活性。

有效期：12個月（-20℃）/6個月（2-8℃）。

檢測波長：450nm。

所需樣本體積：50-100ul。

人白介素10（IL10）elisa試劑盒檢測目的：用于測定血清、血漿及相關液體等樣本（如：血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養(yǎng)上清、組織勻漿等標本）。

供應種屬：人、小鼠、大鼠、豚鼠、兔、豬、犬、牛、羊、雞、鴨、植物等ELISA試劑盒。

標本類型：包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等，注：標本必須為液體，不含沉淀。

ELISA常用的方法：雙抗體夾心法、間接法、競爭法以及BAS-ELISA等。

溫馨提示：將標本及時分裝后放在-20℃或-70℃條件下保存。避免反復凍融。標本2-8℃可保存48小時，-20℃可保存1個月。-70度可保存6個月。

人白介素10（IL10）elisa試劑盒等潤裕生物elisa試劑盒產(chǎn)品優(yōu)勢：

1.專一性強?？乖c抗體的免疫反應是專一反應，而免疫酶技術以免疫反應為基礎，所檢測的對象是抗原（或抗體），使用的抗體除標記了酶以外，與普通抗體的免疫反應特性并無多大差別。

2.靈敏度高。由于抗體聯(lián)結上了酶，因此，借助于酶與底物的顯色反應，顯示抗原與抗體的結合，大大提高了檢測的靈敏性，使檢測水平接近放射免疫測定法。

3.樣品易保存。經(jīng)過酶反應顯示的有色產(chǎn)物大多比較穩(wěn)定，因此有利于樣品的保存。

4.結果易觀察。對檢測結果即可用肉眼觀察，又可用顯微鏡觀察，也可以用分光光度計進行比色測定，還可用顯微鏡觀察。這是因為某些酶反應產(chǎn)物能使電子密度發(fā)生改變，從而引起被檢測物的顯示。

5.可以定量測定。溶液中的抗原物質(zhì)，應用酶免吸附技術進行比色測定，依據(jù)光密度值的變化，可以定量。預計用細胞分光光度計可對組織內(nèi)的抗原進行免疫酶技術的定量測定。

6.可以大規(guī)模測定樣品。如有成千上萬份樣品需要進行檢測，免疫酶技術都能在較短時間內(nèi)完成。

7.儀器和試劑簡單。對免疫沒技術來說，不需要熒光顯微鏡，也不需要測定放射性的特殊儀器，所用儀器及試劑均屬一般性儀器與試劑，普通實驗室及生產(chǎn)應用單位均易購置。

人白介素10（IL10）elisa試劑盒等潤裕生物ELISA試劑盒產(chǎn)品，原裝ELISA試劑盒以及各類國產(chǎn)ELISA試劑盒產(chǎn)品8折優(yōu)惠熱銷中，歡迎廣大科研學者前來選購：RY-(elisa)-00145豬低分子肝素(LMWH)ELISA試劑盒Porcine low molecular weight heparin,LMWH ELISA Kit規(guī)格:96T/48TRY-(elisa)-00146豬巨噬細胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒Porcine macrophage inflammatory protein 1α,MIP-1α ELISA Kit規(guī)格:96T/48TRY-(elisa)-00147豬主要急性期蛋白( MAP)ELISA試劑盒Porcine MAP ELISA Kit規(guī)格:96T/48TRY-(elisa)-00148豬基質(zhì)金屬蛋白酶9(MMP-9)ELISA試劑盒 Porcine matrix metalloproteinase 9,MMP-9 ELISA Kit規(guī)格:96T/48TRY-(elisa)-00149豬黑色素瘤轉(zhuǎn)移表面黏附分子(MMSAM)ELISA試劑盒Porcine melanoma metastasis surface adhesion molecule,MMSAM ELISA Kit規(guī)格:96T/48TRY-(elisa)-00150豬褪黑素(MT)ELISA試劑盒Porcine Melatonin,MT ELISA Kit規(guī)格:96T/48TRY-(elisa)-00151豬高鐵血紅蛋白(MHB)ELISA試劑盒Porcine Methemoglobin,MHB ELISA Kit規(guī)格:96T/48TRY-(elisa)-00152豬髓磷脂堿性蛋白(MBP)ELISA試劑盒Porcine myelin basic protein,MBP ELISA Kit規(guī)格:96T/48TRY-(elisa)-00153豬髓過氧化物酶(MPO)ELISA試劑盒Porcine myeloperoxidase,MPO ELISA Kit規(guī)格:96T/48TRY-(elisa)-00154豬一氧化氮(NO)ELISA試劑盒Porcine nitric oxide,NO ELISA Kit規(guī)格:96T/48TRY-(elisa)-00155豬去甲腎上腺素(NE)ELISA試劑盒Porcine Noradrenaline,NE ELISA Kit規(guī)格:96T/48TRY-(elisa)-00156豬Ⅲ型前膠原肽(PⅢNP)ELISA試劑盒Porcine N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit規(guī)格:96T/48TRY-(elisa)-00157豬食欲素/阿立新B(OX-B)ELISA試劑盒Porcine Orexin B,OX-B ELISA Kit規(guī)格:96T/48TRY-(elisa)-00158豬食欲素受體(OXR)ELISA試劑盒Porcine Orexin receptor,OXR ELISA Kit規(guī)格:96T/48TRY-(elisa)-00159豬骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒Porcine Osteocalcin/Bone gla protein,OT/BGP ELISA kit規(guī)格:96T/48TRY-(elisa)-00160豬氧化低密度脂蛋白(OxLDL)ELISA試劑盒Porcine oxidized lowdensity lipoprotein,OxLDL ELISA Kit規(guī)格:96T/48TRY-(elisa)-00161豬磷酸化細胞外信號調(diào)節(jié)激酶(pERK)ELISA試劑盒Porcine phospho-extracellular signal-regulated kinase,pERK ELISA Kit規(guī)格:96T/48TRY-(elisa)-00162豬纖溶酶原激活物抑制因子1(PAI-1)ELISA試劑盒Porcine plasminogen activator inhibitor 1,PAI-1 ELISA Kit規(guī)格:96T/48TRY-(elisa)-00163豬纖溶酶原激活物抑制因子(PAI)ELISA試劑盒Porcine plasminogen activator inhibitor,PAI ELISA Kit規(guī)格:96T/48TRY-(elisa)-00164豬血小板活化因子(PAF)ELISA試劑盒Porcine platelet activating factor,PAF ELISA Kit規(guī)格:96T/48TRY-(elisa)-00165豬血小板衍生生長因子(PDGF)ELISA試劑盒Porcine platelet-derived growth factor,PDGF ELISA Kit規(guī)格:96T/48TRY-(elisa)-00166豬Ⅲ型前膠原氨基端肽(PⅢNT)ELISA試劑盒Porcine procollagen Ⅲ N-terminal peptide,PⅢNT ELISA Kit規(guī)格:96T/48TRY-(elisa)-00167豬孕激素/孕酮(PROG)ELISA試劑盒Porcine Progesterone,PROG ELISA Kit規(guī)格:96T/48TRY-(elisa)-00168豬肺表面活性物質(zhì)相關蛋白A(SP-A)ELISA試劑盒Porcine Pulmonary surfatcant-associated protein A,SP-A ELISA Kit規(guī)格:96T/48TRY-(elisa)-00169豬松弛肽/松弛素(RLN)ELISA試劑盒Porcine Relaxin,RLN ELISA Kit規(guī)格:96T/48TRY-(elisa)-00170豬鈉-葡萄糖共轉(zhuǎn)運載體1(SGLT1)ELISA試劑盒Porcine sodium-glucose cotransporter 1,SGLT1 ELISA Kit規(guī)格:96T/48TRY-(elisa)-00171豬可溶性E選擇素(sE-selectin)ELISA試劑盒 Porcine soluble E-selectin,sE-selectin ELISA Kit規(guī)格:96T/48TRY-(elisa)-00172豬可溶性細胞間粘附分子1(sICAM-1)ELISA試劑盒Porcine Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit規(guī)格:96T/48TRY-(elisa)-00173豬可溶性P選擇素(sP-Selectin)ELISA試劑盒Porcine soluble P-selectin,sP-Selectin ELISA Kit規(guī)格:96T/48TRY-(elisa)-00174豬可溶性血管內(nèi)皮生長因子受體2(VEGFR-2/sFLK-1)ELISA試劑盒Porcine soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1 ELISA Kit規(guī)格:96T/48T

上海美旋生物科技有限公司竭誠為您提供優(yōu)質(zhì)的elisa試劑盒產(chǎn)品服務，價格實惠，量大更優(yōu)惠，敬請您來電咨詢！

中文名稱：人白介素10(IL-10)ELISA試劑盒

說明書：歡迎來電索取，通常會隨ELISA試劑盒一起寄出。

規(guī)格：48T/96T（僅用于科研,不得用于臨床診斷）

特異性：本試劑盒可同時檢測天然或重組的，且與其他相關蛋白無交叉反應。

特點：靈敏性高、特異性強、重復性好

人白介素10(IL-10)ELISA試劑盒現(xiàn)貨供應，量大更優(yōu)惠，我公司是專業(yè)的高品質(zhì)ELISA試劑盒供應商，經(jīng)過長年發(fā)展已成為全國各大型科研院所ELISA試劑盒指定供應商與戰(zhàn)略合作伙伴，敬請您來電咨詢！

如需人白介素10(IL-10)ELISA試劑盒說明書請點擊網(wǎng)頁“我要詢價”，填寫詢價留言表（↓↓↓），或者通過網(wǎng)頁上我司的聯(lián)系方式與我們聯(lián)系，電話、在線客服QQ、發(fā)送電子郵件均可，美旋生物專業(yè)供應各種類高品質(zhì)Elisa試劑盒，讓您買得舒心，用得滿意，為您的科研工作提供優(yōu)質(zhì)保障！購買前請聯(lián)系我公司銷售人員了解產(chǎn)品的詳細信息及庫存情況，美旋生物祝您工作順利，生活愉快！

人白介素10(IL-10)ELISA試劑盒現(xiàn)貨供應，量大更優(yōu)惠，全國免費送貨上門：

MX-12761 小鼠硫氧化還原蛋白(Trx)ELISA試劑盒  英文名：Mouse Thioredoxin,Trx ELISA Kit  規(guī)格：48T/96TMX-12762 小鼠膠原酶II(Collagenase II)ELISA試劑盒  英文名：Mouse Collagenase Type II ELISA Kit 規(guī)格：48T/96TMX-12763 小鼠膠原酶I(Collagenase I)ELISA試劑盒  英文名：Mouse Collagenase I ELISA Kit 規(guī)格：48T/96TMX-12764 小鼠磷酸化蛋白激酶C(P-PKC)ELISA試劑盒  英文名：Mouse phospho Protein Kinase C,P-PKC ELISA Kit 規(guī)格：48T/96TMX-12765 小鼠磷酸化細胞外信號調(diào)節(jié)激酶(pERK)ELISA試劑盒  英文名：Mouse phospho-extracellular signal-regulated kinase,pERK ELISA Kit 規(guī)格：48T/96TMX-12766 小鼠鈣通道阻滯劑(CCB)ELISA試劑盒  英文名：Mouse calcium channel blockers,CCB ELISA Kit  規(guī)格：48T/96TMX-12767 小鼠丙氨酸轉(zhuǎn)氨酶/谷丙轉(zhuǎn)氨酶(ALT/GPT)ELISA試劑盒  英文名：Mouse Alanine Aminotransferase,ALT/GPT ELISA Kit 規(guī)格：48T/96TMX-12768 小鼠骨形成蛋白(BMPs)ELISA試劑盒  英文名：Mouse bone morphogenetic proteins,BMPs ELISA Kit 規(guī)格：48T/96TMX-12769 小鼠視黃醇結合蛋白4(RBP-4)ELISA試劑盒  英文名：Mouse Retinol-binding protein 4,RBP-4 ELISA Kit 規(guī)格：48T/96TMX-12770 小鼠6酮前列腺素F1a(6-keto-PGF1a)ELISA試劑盒  英文名：Mouse 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit 規(guī)格：48T/96TMX-12771 小鼠戊糖素(Pentosidine)ELISA試劑盒  英文名：Mouse Pentosidine ELISA Kit 規(guī)格：48T/96TMX-12772 小鼠晚期糖基化終末產(chǎn)物(AGEs)ELISA試劑盒 英文名：Mouse advanced glycation end products,AGEs ELISA Kit  規(guī)格：48T/96TMX-12773 小鼠中性粒細胞明膠酶相關脂質(zhì)運載蛋白(NGAL)ELISA試劑盒  英文名：Mouse neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit 規(guī)格：48T/96T

本公司長期供應人白介素10(IL-10)ELISA試劑盒，全方位售前、售中、售后服務，為您解決實驗過程中的問題。

小鼠白介素10（IL-10）酶免測定elisa試劑盒價格,北京現(xiàn)貨代檢測試劑盒種類 種屬 規(guī)格

細胞因子檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

內(nèi)分泌檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

肝纖維化檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

心肌梗塞檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

腫瘤標志物檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

自身免疫檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

優(yōu)生優(yōu)育檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

傳染病檢測試劑盒 人/小鼠/大鼠/其他 48T/96T

ELISA可檢測標本：血清、血漿、細胞上清液、尿液、灌洗液、腦脊髓、心房水、胸腹水、組織等。

Mouse interleukin 10,IL-10 ELISA kit 加拿大HCB公司ELISA試劑盒價格 北京代理,elisa實驗樣本收集：（1）血清（血漿）樣本：取全血加入促凝管（抗凝管），2500轉(zhuǎn)離心20分鐘左右，收集上清，液氮或者-80℃冰箱保存；（2）尿液樣本：樣本2500轉(zhuǎn)離心20分鐘左右，液氮或者-80℃冰箱保存；胸腹水、腦脊液、肺泡灌洗液參照此實行；（3）細胞樣本：檢測分泌性的成份時，樣本2500轉(zhuǎn)離心20分鐘左右，液氮或者-80℃冰箱保存；檢測細胞內(nèi)的成份時，用PBS稀釋細胞懸液，通過反復凍融，以使細胞破壞并放出細胞內(nèi)成份，2500轉(zhuǎn)離心20分鐘左右，收集上清同上；（4）組織樣本：切割標本后，稱取重量，用液氮或者-80℃冰箱冷凍保存?zhèn)溆谩＃?）標本采集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，有條件的，最好-70℃凍存?zhèn)溆?，但應避免反復凍?（6）關于樣本收集更詳細信息，請聯(lián)系我公司技術人員。

小鼠白介素10（IL-10）酶免測定ELISA試劑盒價格,北京現(xiàn)貨代檢測 北京代理-酶聯(lián)免疫試劑盒供貨商 elisa試劑盒供應廠家 可以免費代測 保證質(zhì)量 無效退款！

小鼠解整合素樣金屬蛋白酶8 ELISA試劑盒免費代做,ELISA Kit代測小鼠ADAM8 ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠解整合素樣金屬蛋白酶9 ELISA試劑盒免費代做,ELISA Kit代測小鼠ADAM9 ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書 小鼠乙酰膽堿受體抗體 ELISA試劑盒免費代做,ELISA Kit代測小鼠AChRab ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠乙酰膽堿 ELISA試劑盒免費代做,ELISA Kit代測小鼠ACH ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠酸性磷酸酶 ELISA試劑盒免費代做,ELISA Kit代測小鼠ACP ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠活化蛋白C ELISA試劑盒免費代做,ELISA Kit代測小鼠APC ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書 小鼠活化素A ELISA試劑盒免費代做,ELISA Kit代測小鼠ACV-A ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠脂聯(lián)素 ELISA試劑盒免費代做,ELISA Kit代測小鼠ADP ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠促腎上腺皮質(zhì)激素 ELISA試劑盒免費代做,ELISA Kit代測小鼠ACTH ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書小鼠腎上腺素能a1A受體 ELISA試劑盒免費代做,ELISA Kit代測小鼠ADRA1A ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書 小鼠腎上腺髓質(zhì)素 ELISA試劑盒免費代做,ELISA Kit代測小鼠ADM ELISA檢測方法,elisa試劑盒售價,哪里代測,elisa實驗操作步驟,elisa kit說明書

 Rat Interleukin 10（IL-10）  

ELISA Kit

Catalog No. CSB-E04595r

（96T）

l          This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma ａnd Tissue Homogenates.

l          Expiration date   six months from the date of manufacture

l          FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well ａnd incubated. Then a TMB （3,3＇,5,5＇ tetramethyl-benzidine） substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody ａnd enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.

SPECIFICITY

This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.

SENSITIVITY

The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.

The sensitivity of this assay, or Lower Limit of Detection （LLD） was defined as the lowest protein concentration that could be differentiated from zero.

MATERIALS PROVIDED

STORAGE

1.      Unopened test kits should be stored at 2-8°C upon receipt ａnd the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.

2.      Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above. 

3.      A microtiter plate reader with a bandwidth of 10 nm or less ａnd an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.

REAGENT PREPARATION

Bring all reagents to room temperature before use.

1.        Wash Buffer  If crystals have formed in the concentrate, warm up to room temperature ａnd mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.

2.        Standard  Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard （200 pg/ml）. The Sample Diluent serves as the zero standard （0 pg/ml）. Prepare fresh for each assay. Use within 4 hours ａnd discard after use.

3.        Biotin-antibody  Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent（1:100）, respectively.

4.        HRP-avidin  Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent（1:100）, respectively.

Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, ａnd clothing protection when using this material.

OTHER SUPPLIES REQUIRED

—          Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

—          Pipettes ａnd pipette tips.

—          Deionized or distilled water.

—          Squirt bottle, manifold dispenser, or automated microplate washer.

—          An incubator which can provide stable incubation conditions up to 37°C±0.5°C.

SAMPLE COLLECTION AND STORAGE

l          Serum  Use a serum separator tube （SST） ａnd allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum ａnd assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Plasma  Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot ａnd store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Tissue Homogenates   100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS ａnd stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed ａnd removed immediately. Alternatively, aliquot ａnd store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

ASSAY PROCEDURE

Bring all reagents ａnd samples to room temperature before use. It is recommended that all samples, standards, ａnd controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.

1.        Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.

2.        Remove the liquid of each well, don’t wash.

3.        Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature ａnd mix gently until solution appears uniform.

4.        Aspirate each well ａnd wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer （200μl） ａnd let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.

5.        Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.

6.        Repeat the aspiration ａnd wash five times as step 4.

7.        Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts ａnd other temperature fluctuations in the dark.

8.        Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.

9.        Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.

CALCULATION OF RESULTS

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.

Average the duplicate readings for each standard, control, ａnd sample ａnd subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ａnd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. ａnd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

LIMITATIONS OF THE PROCEDURE

—          The kit should not be used beyond the expiration date on the kit label.

—          Do not mix or substitute reagents with those from other lots or sources.

—          It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.

—          If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent ａnd repeat the assay.

—          Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, ａnd kit age can cause variation in binding.

—          This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

TECHNICAL HINTS

—          Centrifuge vials before opening to collect contents.

—          When mixing or reconstituting protein solutions, always avoid foaming.

—          To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, ａnd between reagent additions. Also, use separate reservoirs for each reagent.

—          When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.

—          To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

—          Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.

—          Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.