1色譜條件:色譜柱:Shim—Pack ODS一18 (150x4.6mm);流動相:甲醇一水一36%醋酸(65: 28:7);檢測波長:252nm;流速:1.2ml/min。
2.1.3線性關(guān)系:精密稱取128.4ling(折算成純 MAG25.62mg)置25ml量瓶,加適量甲醇溶解, 并定容,搖勻。分別吸取上述溶液0.5、1.0、 2.0、3.0、4.0Ⅱd置各5Ⅱd量瓶中,用甲醇定容。 吸取10 l,進(jìn)樣測定,以峰面積(y)為縱坐標(biāo), MAG量㈣為橫坐標(biāo),進(jìn)行線性回歸,得回歸方程:Y=I.344xlO 一9.149xl@(r=0.9999)。結(jié) 果表明MAG進(jìn)樣量在1.0-8.0 g范圍內(nèi)線性 關(guān)系良好。
2.1.4回收率試驗(yàn):采用加樣回收法。精密吸 取已知含量(0.389mg/m1)的同批號樣品6份, 每份2.0ml,分別精確加入已知濃度(0.405mg/ m1)的MAG溶液2.0ml,混勻。精密吸取10pJ,, 按上述色譜條件進(jìn)行測定,測得6份樣品的 平均回收率為95.6%,RSD1.4%。
2.2.2制備分離:3用流動相配制成20mg/ml的 溶液,進(jìn)樣30ml,收集各流份。用HPLC分析, 確定所要流份。重復(fù)進(jìn)樣,合并所要流份,減 壓濃縮至近干。加冰醋酸重結(jié)晶。得到針狀 結(jié)晶物。真空干燥,得樣品2。
2.3結(jié)構(gòu)確證熔點(diǎn)測定,溫度計(jì)未經(jīng)校正; 元素分析儀為CARLO ERBA 1 106型;熱重分 析(TGA)用Perkin—Elmer 7 Series Thermal Analysis System;紅外光譜儀為Shimadzu IR- 460型,KBr壓片;核磁共振儀為Bruker AM一 400型,以TMS為內(nèi)標(biāo),CD3OD為溶劑;質(zhì)譜儀 為Varian MAT-711型。
實(shí)驗(yàn)中曾比較其他流動相(1)甲醇一水 36%醋酸(69:31:O.5);(2)乙腈-2%醋酸(4:6): (3)氫氧化四丁基銨溶液(3.2--)1000)-~醇一乙 腈(60:30:12),以磷酸調(diào)pH值6.0。結(jié)果以甲 醇一水一36%醋酸(65:28:7)流動相的分離效果 為最佳。因MAG甲醇溶液的最大吸收波長在 252nm處,故選擇252nm作為檢測波長。
品質(zhì)保證:收到貨后有問題包退包換,實(shí)驗(yàn)過程中有問題我們?yōu)槟峁┘夹g(shù)支持(3β,6α,16β,20R,24S)-3-O-[(3,4-二乙?;?β-D-木糖)]-20, 24-環(huán)氧-16,25-二羥基-9,19-環(huán)羊毛甾烷-6-O-葡萄糖苷其它同類產(chǎn)品如下:(R型)人參皂苷Rh1Ginsenoside Rh1HPLC≥98%20mg/支800.00
(R型)人參皂苷Rh2Ginsenoside Rh2112246-15-8HPLC≥96%20mg/支800.00
人參皂苷Rh4Ginsenoside Rh4174721-08-5HPLC≥98%10mg/支2000.00
人參皂苷Rh7Gensenoside Rh7343780-68-7HPLC≥98%10mg/支3800.00
人參皂苷Rh8Ginsenoside Rh8343780-69-8HPLC≥98%5mg/支3800.00
(R型)人參皂苷Rg2Ginsenoside Rg280952-72-3HPLC≥98%20mg/支1600.00
(R型)人參皂苷Rg3Ginsenoside Rg338243-03-7HPLC≥98%20mg/支800.00
(R型)原人參三醇Protopanaxtriol1453-93-6HPLC≥98%20mg/支1600.00
人參皂苷F1Ginsenoside F153963-43-2HPLC≥98%20mg/支900.00
人參皂苷F2Ginsenoside F262025-49-4HPLC≥98%20mg/支900.00
人參皂苷F3Ginsenoside F362025-50-7HPLC≥98%10mg/支880.00
人參皂苷F4Ginsenoside F4181225-33-2HPLC≥98%10mg/支2000.00
人參皂苷F5Ginsenoside F5189513-26-6HPLC≥98%20mg/支2000.00
人參皂苷CKCompound K39262-14-1HPLC≥98%20mg/支800.00
(R型)原人參二醇Protopanaxdiol30636-90-9HPLC≥95%20mg/支800.00
人參皂苷RoGinsenoside Ro34367-04-9HPLC≥98%20mg/支780.00
人參皂苷Rg5Ginsenoside Rg5186763-78-0HPLC≥98%10mg/支2800.00
人參皂苷Rg6Ginsenoside Rg6HPLC≥98%20mg/支2500.00
人參皂苷Rk1Ginsenoside Rk1494753-69-4HPLC≥98%20mg/支2500.00
人參皂苷Rk2Ginsenoside Rk2364779-14-6HPLC≥98%20mg/支
人參皂苷Rk3Ginsenoside Rk3364779-15-7HPLC≥98%10mg/支2800.00
人參二醇Panaxadiol19666-76-3HPLC≥98%20mg/支120.00
人參三醇Panaxtriol32791-84-7HPLC≥98%20mg/支120.00
(S型)原人參二醇Protopanaxdiol30636-90-9HPLC≥98%20mg/支300.00
三七皂苷FeNotoginsenoside Fe88105-29-7HPLC≥98%20mg/支1200.00
越南參皂苷R3Vina-ginsenoside R3156012-92-9HPLC≥95%10mg/支3800.00
越南參皂苷R4Vina-ginsenoside R4156009-80-2HPLC≥98%20mg/支2600.00
越南參皂苷R8Vina-ginsenoside R8156042-22-7HPLC≥98%10mg/支2200.00
柴胡皂苷ASaikosaponin A20736-09-8HPLC≥98%20mg/支400.00
柴胡皂苷CSaikosaponin C20736-08-7HPLC≥98%20mg/支700.00
柴胡皂苷DSaikosaponin D20874-52-6HPLC≥98%20mg/支600.00
柴胡皂苷BSaikosaponin BHPLC≥98%20mg/支
柴胡皂苷B1Saikosaponin B158558-08-0HPLC≥98%20mg/支1200.00
柴胡皂苷B2Saikosaponin B258316-41-9HPLC≥98%20mg/支800.00
山奈苷Kaempferitrin;Lespedin; Lespenephryl482-38-2HPLC≥98%20mg/支700.00
三七皂苷R1Notoginsenoside R180418-24-2HPLC≥98%20mg/支200.00
三七皂苷Ft1Notoginsenoside Ft1HPLC≥98%20mg/支800.00
三七皂苷R2(R型)20(S)-Notoginsenoside R2948046-15-9HPLC≥98%20mg/支2200.00
三七皂苷R2(S型)20(S)-NotoginsenosideR280418-25-3HPLC≥98%20mg/支2500.00
三七總皂苷Standard100mg/支100.00
澤瀉醇AAlisol A19885-10-0HPLC≥98%20mg/支1480.00
澤瀉醇BAlisol B18649-93-9HPLC≥98%20mg/支1400.00
澤瀉醇A-24-醋酸酯;
24-乙酰澤瀉醇AAlisol A 24-acetate18674-16-3HPLC≥98%20mg/支1280.00
25-甲氧基澤瀉醇A25-Methoxyalisol A155801-00-6HPLC≥98%10mg/支2600.00
澤瀉醇B-23醋酸酯;
23-乙酰澤瀉醇BAlisol B 23-acetate26575-95-1HPLC≥98%20mg/支780.00
23-乙酰澤瀉醇C23-Acetyl alisol C26575-93-9HPLC≥98%20mg/支2400.00
澤瀉醇FAlisol F155521-45-2HPLC≥98%20mg/支2680.00
25-脫水澤瀉醇A;
澤瀉醇GAlisol G155521-46-3HPLC≥98%10mg/支2200.00
24-乙酰澤瀉醇FAlisol F 24-acetate443683-76-9HPLC≥97%5mg/支2800.00
環(huán)氧澤瀉烯Alismoxide87701-68-6HPLC≥98%20mg/支1400.00
α-常春藤皂苷α-Hederin27013-91-8HPLC≥98%20mg/支1280.00
白頭翁皂苷A3Pulchinenoside A3129724-84-1HPLC≥98%20mg/支980.00
白頭翁皂苷B4Anemoside B4129741-57-7HPLC≥98%20mg/支980.00
常春藤苷C;
常春藤皂苷CHederacoside C14216-03-6HPLC≥98%20mg/支1000.00
白頭翁皂苷BPulchinenoside B135247-95-9HPLC≥98%2mg/支2980.00
白頭翁皂苷D;
3-O-β-D-葡萄糖( 1→4)-[ -L-鼠李糖(1→2)]- -L-阿拉伯糖 23-羥基羽扇豆20(29)-烯-28–酸3-O-D-glucopyranosyl( 1→4)- [ L-rhamnopyranosyl(1→2)]-L-arabinopyranosyl 23-hydroxyl lup-20(29)-en-28-oic acid – 28-O-rhamnopyranosyl(1→4)glucopyranosyl(1→6)glucopyranoside848784-85-0HPLC≥98%5mg/支2980.00
白頭翁皂苷E1;
常春藤配基-3-O-β-D-半乳糖( 1→4)β-D葡萄糖( 1→3)-α-L-鼠李糖(1→2)-α-L-阿拉伯糖苷Hederagenin - 3-O-β-D- Galactosyl( 1→3)-β-D-glucosyl( 1→3)-α-L-rhamnosyl(1→2)-α-L-arabinosideHPLC≥98%10mg/支2980.00
白頭翁皂苷E2;
齊墩果酸-3-O-β-D-半乳糖( 1→4)β-D葡萄糖( 1→3)-α-L-鼠李糖(1→2)-α-L-阿拉伯糖苷Oleanolic acid - 3-O-β-D- Galactosyl( 1→3)-β-D-glucosyl( 1→3)-α-L-rhamnosyl(1→2)-α-L-arabinosideHPLC≥98%10mg/支2980.00
白頭翁皂苷E3;
3-O-β-D-半乳糖( 1→4)β-D葡萄糖( 1→3)-α-L-鼠李糖(1→2)-α-L-阿拉伯糖-齊墩果酸-28-O-α-L-鼠李糖(1→4)β-D-葡萄糖( 1→6)-β-D-葡萄糖苷3-O-β-D- Galactosyl( 1→3)-β-D-glucosyl( 1→3)-α-L-rhamnosyl(1→2)-α-L-arabinosyl-Oleanolic acid - 28-O-α-L-rhamnosyl(1→4)β-D-glucosyl( 1→6)-β-D-glucosideHPLC≥98%10mg/支2980.00
白頭翁皂苷E4;
齊墩果酸-3-O-α-L-鼠李糖(1→6)β-D-半乳糖( 1→4)β-D葡萄糖( 1→3)-α-L-鼠李糖(1→2)-α-L-阿拉伯糖苷Oleanolic acid - 3-O-α-L-rhamnosyl(1→6)β-D- Galactosyl( 1→3)-β-D-glucosyl( 1→3)-α-L-rhamnosyl(1→2)-α-L-arabinosideHPLC≥98%5mg/支2980.00
常春藤苷H;
灰氈毛忍冬次皂苷甲Kalopanaxsaponin H128730-82-5HPLC≥98%5mg/支2980.00
Hederacolchiside A1106577-39-3HPLC≥98%5mg/支2980.00
Hederacolchiside E33783-82-3HPLC≥98%5mg/支2980.00
苦蘇花皂苷CCussosaponin C366814-42-8HPLC≥98%5mg/支2980.00
齊墩果酸-3-O-β-D葡萄糖( 1→3)-α-L-鼠李糖(1→2)-α-L-阿拉伯糖苷Oleanolic acid 3-O-β-D-glucosyl-( 1→3)-α-L-ramnosyl(1→2)-α-L-arabinoside103956-33-8HPLC≥98%5mg/支2980.00
Lup-20(29)-en-28-oic acid, 3-[ D-glucopyranosyl(1→4)[ L-rhamnopyranosyl) (1→2)-L-arabinopyranosyl]oxy], (3,4)-)848784-87-2HPLC≥98%5mg/支2980.00
Hederagenin 3-O-α-L-rhamnopyranosyl(1→2)-(β-D-glucopyranosyl(1→4))-α-L-arabinopyranoside68027-15-6HPLC≥98%5mg/支2980.00
3-O-β-D-葡萄糖( 1→3)- -L-鼠李糖(1→2)- -L-阿拉伯糖 齊墩果酸– 28-O-鼠李糖(1→4)葡萄糖(1→6)葡萄糖苷3-O-D-glucopyranosyl( 1→3)-L-rhamnopyranosyl(1→2)-L-arabinopyranosyl Oleanolic acid – 28-O-rhamnopyranosyl(1→4)glucopyranosyl(1→6)glucopyranoside261767-91-3HPLC≥98%5mg/支2980.00
白頭翁皂苷E;
3-O--D-葡萄糖( 1→4)-[ -L-鼠李糖(1→2)]- -L-阿拉伯糖 23-羥基羽扇豆20(29)-烯-28–酸- 28-O-鼠李糖(1→4)葡萄糖(1→6)葡萄糖苷3-O-D-glucopyranosyl( 1→3)-L-rhamnopyranosyl(1→2)-L-arabinopyranosyl lupinic acid– 28-O-rhamnopyranosyl(1→4)glucopyranosyl(1→6)glucopyranoside366814-43-9HPLC≥98%5mg/支2980.00
白頭翁皂苷H;
3-O-β-D-葡萄糖( 1→4)-[ -L-鼠李糖(1→2)]- -L-阿拉伯糖 常春藤配基- 28-O-鼠李糖(1→4)葡萄糖(1→6)葡萄糖苷3-O-D-glucopyranosyl( 1→4)- [ L-rhamnopyranosyl(1→2)]-L-arabinopyranosyl 23-hydroxyl lup-20(29)-en-28-oic acid – 28-O-rhamnopyranosyl(1→4)glucopyranosyl(1→6)glucopyranoside68027-14-5HPLC≥98%5mg/支2980.00
黃花敗醬甙C;
牡丹草苷BScabioside C17233-22-6HPLC≥98%5mg/支2980.00
6-姜酚;
姜辣素;
6-姜辣素6-gingerol23513-14-6HPLC≥98%20mg/支760.00
8-姜酚8-Gingerol23513-08-8; 30462-35-2HPLC≥98%20mg/支980.00
10-姜酚10-Gingerol23513-15-7HPLC≥98%20mg/支980.00
6-姜烯酚6-Shogaol555-66-8HPLC≥98%20mg/支1600.00
8-姜烯酚8-Shogaol36700-45-5HPLC≥98%20mg/支2600.00
姜酮Zingerone122-48-5HPLC≥98%20mg/支680.00
麥冬甲基黃烷酮A;
甲基麥冬二氫高異黃酮AMethylophiopogonanone A74805-92-8HPLC≥98%20mg/支1680.00
山麥冬皂苷BLiriopesides B182284-68-0HPLC≥98%20mg/支1800.00
短葶山麥冬皂苷CLiriope muscari baily saponins C87480-46-4HPLC≥98%20mg/支600.00
魯斯可皂苷元;
魯斯考皂苷元Ruscogenin472-11-7HPLC≥98%20mg/支980.00
麥冬皂苷DOphiopogonin D41753-55-3HPLC≥98%20mg/支1480.00
Ophiopojaponin C911819-08-4HPLC≥98%5mg/支2480.00
3-O-α-L-鼠李糖-(1→2)-β-葡萄糖麥冬苷元Saponin VHPLC≥98%10mg/支2480.00
薯蕷皂苷元-3-O-β-D-木糖-(1→3) -β-D-葡萄糖苷Diosgenin-3-O-β-D-xylosyl-(1→3)-β-glucoside2mg/支2480.00
去乙?;鵒phiopojaponin ADeacetyl ophiopojaponin A313054-32-95mg/支2480.00
14α-羥基Sprengerinin C14α-hydroxy Sprengerinin C;
Saponin U1111088-89-1HPLC≥98%5mg/支2480.00