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電線(xiàn)桿裂縫寬度測(cè)試儀主要技術(shù)參數(shù)：

主機(jī)參數(shù)屏幕尺寸：5英寸 液晶分辨率：160×128  體積：195×140×45mm 重量：0.8kg主機(jī)外殼防水、防塵、防震測(cè)量范圍0.01 ~ 2.00mm讀數(shù)精度0.005mm放大倍數(shù)40倍最小分度0.02mm供電方式內(nèi)置鋰電池連接線(xiàn)長(zhǎng)2.5m主機(jī)重量0.8kg工作環(huán)境溫度-10℃～40℃包裝規(guī)格材質(zhì)：工程塑料體積：×315×170mm 重量：5kg 

 Rat Interleukin 10（IL-10）  

ELISA Kit

Catalog No. CSB-E04595r

（96T）

l          This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma ａnd Tissue Homogenates.

l          Expiration date   six months from the date of manufacture

l          FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well ａnd incubated. Then a TMB （3,3＇,5,5＇ tetramethyl-benzidine） substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody ａnd enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.

SPECIFICITY

This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.

SENSITIVITY

The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.

The sensitivity of this assay, or Lower Limit of Detection （LLD） was defined as the lowest protein concentration that could be differentiated from zero.

MATERIALS PROVIDED

STORAGE

1.      Unopened test kits should be stored at 2-8°C upon receipt ａnd the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.

2.      Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above. 

3.      A microtiter plate reader with a bandwidth of 10 nm or less ａnd an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.

REAGENT PREPARATION

Bring all reagents to room temperature before use.

1.        Wash Buffer  If crystals have formed in the concentrate, warm up to room temperature ａnd mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.

2.        Standard  Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard （200 pg/ml）. The Sample Diluent serves as the zero standard （0 pg/ml）. Prepare fresh for each assay. Use within 4 hours ａnd discard after use.

3.        Biotin-antibody  Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent（1:100）, respectively.

4.        HRP-avidin  Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent（1:100）, respectively.

Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, ａnd clothing protection when using this material.

OTHER SUPPLIES REQUIRED

—          Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

—          Pipettes ａnd pipette tips.

—          Deionized or distilled water.

—          Squirt bottle, manifold dispenser, or automated microplate washer.

—          An incubator which can provide stable incubation conditions up to 37°C±0.5°C.

SAMPLE COLLECTION AND STORAGE

l          Serum  Use a serum separator tube （SST） ａnd allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum ａnd assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Plasma  Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot ａnd store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Tissue Homogenates   100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS ａnd stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed ａnd removed immediately. Alternatively, aliquot ａnd store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

ASSAY PROCEDURE

Bring all reagents ａnd samples to room temperature before use. It is recommended that all samples, standards, ａnd controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.

1.        Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.

2.        Remove the liquid of each well, don’t wash.

3.        Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature ａnd mix gently until solution appears uniform.

4.        Aspirate each well ａnd wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer （200μl） ａnd let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.

5.        Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.

6.        Repeat the aspiration ａnd wash five times as step 4.

7.        Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts ａnd other temperature fluctuations in the dark.

8.        Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.

9.        Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.

CALCULATION OF RESULTS

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.

Average the duplicate readings for each standard, control, ａnd sample ａnd subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ａnd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. ａnd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

LIMITATIONS OF THE PROCEDURE

—          The kit should not be used beyond the expiration date on the kit label.

—          Do not mix or substitute reagents with those from other lots or sources.

—          It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.

—          If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent ａnd repeat the assay.

—          Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, ａnd kit age can cause variation in binding.

—          This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

TECHNICAL HINTS

—          Centrifuge vials before opening to collect contents.

—          When mixing or reconstituting protein solutions, always avoid foaming.

—          To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, ａnd between reagent additions. Also, use separate reservoirs for each reagent.

—          When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.

—          To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

—          Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.

—          Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

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RJ518050旋轉(zhuǎn)關(guān)節(jié)產(chǎn)品特征：

 [DC to 40 GHz] 

0-40GHz

K型接頭

接觸式單路同軸旋轉(zhuǎn)關(guān)節(jié)，可傳輸直流電

 

技術(shù)參數(shù)：

Specifications
Interface Type	2.92 Female ( 50 ohms )	Style	I Type
Frequency Range	DC to 40 GHz	Phase WOW (Degrees Max.)	1°
VSWR, Max.	1.30  @  DC  to  10 GHz	Insertion Loss, ,Max.	0.25 dB @ DC  to  10 GHz
	1.45  @ 10  to  20 GHz		0.40 dB  @ 10  to  20 GHz
	1.60  @ 20  to  30 GHz		0.55 dB  @ 20  to  30 GHz
	1.75  @ 30  to  40 GHz		0.70 dB  @ 30  to  40 GHz
VSWR WOW	0.1	Insertion Loss WOW	0.02 dB
Peak Power, Max.	500 W	Average Power, Max.	60 W @ 1 GHz
Rotating Speed, Max.	250  rpm	Life Time, Min.	5 Million Revolutions
Starting Torque	3.5 Ncm.	Continuous Rotational Torque	3.5 Ncm Max.
Axial Load On Interface, Max	± 1 N	Radial Load On Interface, Max.	± 1  N
Body Material	Stainless Steel	Contact Pin Material	Beryllium Copper
DC Power Ability	3 A * 24V @ Full RF Power	Contact Surface Finish	Gold Plate
Weight	0.014 kg	Marking	Adhesive Label
Insulator Material	PTFE / Teflon	IP Protection Level	IP 64 Per EN 60529
Temperature(Ambient Range)	-40  to +60 °C (Operation)	Humidity	85%  (Operation)
	-50  to +70 °C (Storage)	(Non-Condensing), Max.	95%  (Storage)

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KEM自動(dòng)滴定儀AT-710系列KEM自動(dòng)滴定儀AT-710系列主要特點(diǎn):AT-710M:觸摸屏和操作單元之間無(wú)線(xiàn)連接，同時(shí)控制四臺(tái)自動(dòng)滴定測(cè)量單元。通過(guò)使用無(wú)線(xiàn)適配器(藍(lán)牙)，操作單元和滴定裝置可以不用電纜連接。在測(cè)量樣品過(guò)程中有危害性氣體時(shí)，可將滴定裝置放置在排煙柜中，由無(wú)線(xiàn)傳輸?shù)牟僮鲉卧獔?zhí)行滴定。AT-710M/AT-710S:滴定劑的信息存儲(chǔ)在滴定管上的芯片中。即使滴定管單元轉(zhuǎn)移到另一個(gè)滴定裝置,也不需要重新輸入滴定劑信息。AT-710M/AT-710S/AT-710B:滴定管單元特殊的開(kāi)關(guān)閥門(mén)。減少滴定管和開(kāi)關(guān)閥門(mén)間的死體積。特殊結(jié)構(gòu)的設(shè)計(jì)可減少滴定劑的使用量和快速更換滴定單元。AT-710M/AT-710S/AT-710B:智能化電極電纜。電極的信息可透過(guò)電纜上傳感器存儲(chǔ)相關(guān)資料。電極校準(zhǔn)的數(shù)據(jù)可應(yīng)用到不同的自動(dòng)滴定儀上。KEM自動(dòng)滴定儀AT-710系列技術(shù)參數(shù):

BQS2KW排污排沙潛水泵

產(chǎn)品介紹    BQS（W）礦用隔爆型排污排沙潛水電泵，是泵和電動(dòng)機(jī)組合一體的電力排灌設(shè)備，外型美觀，結(jié)構(gòu)簡(jiǎn)單，密封性能好，性能穩(wěn)定，壽命長(zhǎng)，安裝使用方便，且可以長(zhǎng)期在水下連續(xù)作業(yè)，被廣泛用于煤礦立井、斜井及井底散煤泥地自動(dòng)化排水。內(nèi)裝式電泵，水由電動(dòng)機(jī)夾層中流過(guò)，有效的延長(zhǎng)了電泵的使用壽命；外裝式電泵，葉輪采用雙流道大過(guò)流結(jié)構(gòu)，運(yùn)行平穩(wěn)，排污能力強(qiáng)，防纏繞，無(wú)堵塞現(xiàn)象，并且有體積小，重量輕，攜帶方便的特點(diǎn)。結(jié)構(gòu)特點(diǎn)    BQS（W）系列的礦用隔爆型排污排沙潛水電泵嚴(yán)格根據(jù)煤炭行業(yè)標(biāo)準(zhǔn)MT/T671-2005《煤礦用隔爆型潛水電泵》、中華人名共和國(guó)國(guó)家執(zhí)行標(biāo)準(zhǔn)GB3836-2000《爆炸性氣體環(huán)境用電氣設(shè)備》設(shè)計(jì)制作，電動(dòng)機(jī)采用Y型干式隔爆型三相異步電動(dòng)機(jī)，具有啟動(dòng)轉(zhuǎn)矩大、運(yùn)行性能好、噪聲低、體積小、重量輕、維護(hù)方便等特點(diǎn)，以及優(yōu)良的隔爆結(jié)構(gòu)性能和較高的防護(hù)等級(jí)，適用于含有甲烷或煤塵等爆炸性氣體環(huán)境、有爆炸危險(xiǎn)的礦井下采掘面及巷道等場(chǎng)所與水泵配套使用。電泵采用O形橡膠密封圈作為靜密封，結(jié)構(gòu)性能好，采用橡膠硫化曲線(xiàn)控制技術(shù)保障了O型圈耐潮防老化的質(zhì)量及密封性能。動(dòng)密封采用兩套機(jī)械密封，并有油室隔離保護(hù)，有效地為轉(zhuǎn)動(dòng)摩擦副提供潤(rùn)滑，形成流體膜潤(rùn)滑保護(hù)，同時(shí)釋放在運(yùn)動(dòng)過(guò)程中積聚的熱量，從而延長(zhǎng)了機(jī)械密封的使用壽命，確保了密封性能。電泵的流體部件材質(zhì)采用耐磨合金鑄鐵，水泵部分采用流體機(jī)械設(shè)計(jì)及平衡技術(shù)，有效的延長(zhǎng)了電泵的使用壽命。用途含有甲烷或煤塵爆炸危險(xiǎn)的煤礦井下采掘工作面排水，煤礦立井、斜井施工排水，礦井無(wú)水倉(cāng)井底主排水，主井井底散煤倉(cāng)自動(dòng)排水，老塘、無(wú)人區(qū)涌水自動(dòng)排水，煤礦井下含有煤塵、泥沙等較小固體顆粒的地下水排放，清水倉(cāng)排水，救災(zāi)搶險(xiǎn)排水，城市環(huán)境系統(tǒng)中污水排放處理使用條件輸送介質(zhì)溫度應(yīng)不超過(guò)40℃，含固體顆粒的介質(zhì)、其體積濃度一般不超過(guò)2%，輸送介質(zhì)pH值在4~10范圍內(nèi)，工作環(huán)境溫度0~40℃，水泵潛入水深值不超過(guò)5m，介質(zhì)中固體最大粒直徑不大于水泵流道過(guò)流斷面最小尺寸的50%基本參數(shù)

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傳真:  QQ:598298090

網(wǎng)站：http://www.jnzhuoxin.com

CKB型（B200型）單向楔塊超越離合器

特性及應(yīng)用

IKA RCT磁力攪拌器產(chǎn)品優(yōu)勢(shì)新一代最為暢銷(xiāo)的實(shí)驗(yàn)室儀器。New: 強(qiáng)力馬達(dá)，轉(zhuǎn)速范圍廣New: 雙重溫度控制模式用于快速加熱介質(zhì)

IKA RCT磁力攪拌器規(guī)格參數(shù)

訂購(gòu)電話(huà) 13679255673