人BJP ELISA試劑盒 人本周蛋白(BJP) ELISA試劑盒 Human Bence-Jones protein,BJP ELISA試劑盒 96T/48T人胰蛋白酶原激活肽(TAP)ELISA Human trypsinogen activation peptide,TAP ELISA試劑盒 96T/48T人α1酸性糖蛋白(α1-AGP)ELISA試劑盒 Human α1-Acid glycoprotein,α1-AGP ELISA試劑盒 96T/48T人內(nèi)皮型一氧化氮合成酶3(eNOS-3)ELISA試劑盒 Human α1-Acid glycoprotein,α1-AGP ELISA試劑盒 96T/48T人丙二醛(MDA)ELISA試劑盒 Human malondialchehyche,MDA ELISA試劑盒 96T/48T人胰淀素(Amylin)ELISA試劑盒 Human Amylin ELISA試劑盒 96T/48T人血管活性腸肽(VIP)ELISA試劑盒 Human Motilin,MTL ELISA試劑盒 96T/48T人本周蛋白(BJP) ELISA試劑盒 人BJP ELISA試劑盒 Human Bence-Jones protein,BJP ELISA試劑盒 96T/48T人膽囊收縮素/腸促胰酶肽(CCK)ELISA試劑盒 Human cholecystokinin,CCK ELISA試劑盒 96T/48T人Ⅲ型前膠原肽(PⅢNP)ELISA試劑盒 Human N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA試劑盒 96T/48T產(chǎn)品范圍: 人,小鼠,大鼠,豬,兔,其它動(dòng)物細(xì)胞因子;細(xì)胞凋亡,活性多肽、自身抗體 ,血栓與止血, 骨代謝,肝纖維化,腫瘤,激素內(nèi)分泌、自身抗體科研ELISA檢測(cè)試劑盒,提供免費(fèi)代測(cè)服務(wù)。本產(chǎn)品僅供科學(xué)研究。人Ⅱ型膠原(Col Ⅱ)ELISA試劑盒 Human Collagen Type Ⅱ,Col Ⅱ ELISA試劑盒 96T/48T人Ⅰ型膠原(Col Ⅰ)ELISA試劑盒 Human Collagen Type Ⅰ,Col Ⅰ ELISA試劑盒 96T/48T人本周蛋白(BJP) ELISA試劑盒 人BJP ELISA試劑盒 Human Bence-Jones protein,BJP ELISA試劑盒 96T/48T人Ⅰ型前膠原羧基端肽(PⅠCP)ELISA試劑盒 Human procollagen Ⅲ N-terminal peptide,PⅢNT ELISA試劑盒 96T/48T人透明質(zhì)酸(HA)ELISA試劑盒 Human Hyaluronic acid,HA ELISA試劑盒 96T/48T反應(yīng)試劑:在現(xiàn)在ELISA商品試劑盒中,血清樣本的加入幾乎是的要使用微量加樣器加入樣本的步驟,使用微量加樣器加樣必須注意避免以下幾點(diǎn):加樣太快,無(wú)法微量加樣的性和均一性。加在孔壁上部的非包被區(qū),易導(dǎo)致非特異吸附。濺出會(huì)對(duì)鄰近孔產(chǎn)生污染。出現(xiàn)氣泡則反應(yīng)液界面有差異,試劑的加入除了要注意低價(jià)的角度外,滴加的速度也很重要。滴加太快易出現(xiàn)重復(fù)滴加或兩孔之間的現(xiàn)象,這樣就會(huì)在孔內(nèi)的非包被區(qū)出現(xiàn)非特異吸附,從而引起非特異顯色。故有一份標(biāo)本用的試劑盒兩次測(cè)定結(jié)果不不同,往往是上述加樣及試劑的錯(cuò)誤所致。人BJP ELISA試劑盒
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ENGINEERING產(chǎn)品名:LVSH-204G-D1-NO規(guī)格:LVSH-204G-D1-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-D2-NO規(guī)格:LVSH-204G-D2-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-D1-NO-TB規(guī)格:LVSH-204G-D1-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-D2-NO-TB規(guī)格:LVSH-204G-D2-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-A220-TB-ML規(guī)格:LVSH-204G-A220-TB-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A1-TB規(guī)格:LVSH-206G-A1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A2-TB規(guī)格:LVSH-206G-A2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A1D-TB規(guī)格:LVSH-206G-A1D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A1D-NO-TB規(guī)格:LVSH-206G-A1D-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A2D-NO-TB規(guī)格:LVSH-206G-A2D-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-D1-TB規(guī)格:LVSH-206G-D1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-D2-TB規(guī)格:LVSH-206G-D2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-D1-NO-TB規(guī)格:LVSH-206G-D1-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-D2-NO-TB規(guī)格:LVSH-206G-D2-NO-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-206G-A220-TB-ML規(guī)格:LVSH-206G-A220-TB-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-502G-A1D-TB規(guī)格:LVSH-502G-A1D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-502G-A2D-TB規(guī)格:LVSH-502G-A2D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-502G-D1-TB規(guī)格:LVSH-502G-D1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-502G-D2-TB規(guī)格:LVSH-502G-D2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-503G-A1D-TB規(guī)格:LVSH-503G-A1D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-503G-A2D-TB規(guī)格:LVSH-503G-A2D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-503G-D1-TB規(guī)格:LVSH-503G-D1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-503G-D2-TB規(guī)格:LVSH-503G-D2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-504G-A1D-TB規(guī)格:LVSH-504G-A1D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-504G-A2D-TB規(guī)格:LVSH-504G-A2D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-504G-D1-TB規(guī)格:LVSH-504G-D1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-504G-D2-TB規(guī)格:LVSH-504G-D2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-503G-WA1D-ML規(guī)格:LVSH-503G-WA1D-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-A2TB規(guī)格:LVSO-103G-A2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-A1規(guī)格:LVSO-103G-A1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-A2規(guī)格:LVSO-103G-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-A1TB規(guī)格:LVSO-103G-A1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-A2TB規(guī)格:LVSO-103G-A2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-D1規(guī)格:LVSO-103G-D1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-D2規(guī)格:LVSO-103G-D2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-D1TB規(guī)格:LVSO-103G-D1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-103G-D2TB規(guī)格:LVSO-103G-D2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-A1規(guī)格:LVSO-104G-A1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-A2規(guī)格:LVSO-104G-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-A1TB規(guī)格:LVSO-104G-A1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-A2TB規(guī)格:LVSO-104G-A2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-D1規(guī)格:LVSO-104G-D1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-D2規(guī)格:LVSO-104G-D2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-D1TB規(guī)格:LVSO-104G-D1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-104G-D2TB規(guī)格:LVSO-104G-D2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202G-WA1規(guī)格:LVSO-202G-WA1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202G-WA1-S規(guī)格:LVSO-202G-WA1-S品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202G-WA2規(guī)格:LVSO-202G-WA2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202G-WD1規(guī)格:LVSO-202G-WD1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202G-WD2規(guī)格:LVSO-202G-WD2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202T-WA1規(guī)格:LVSO-202T-WA1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202T-WA2規(guī)格:LVSO-202T-WA2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202T-WD1規(guī)格:LVSO-202T-WD1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-202T-WD2規(guī)格:LVSO-202T-WD2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-A1規(guī)格:LVSO-303T-A1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-A2規(guī)格:LVSO-303T-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-A1TB規(guī)格:LVSO-303T-A1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-A2-TB規(guī)格:LVSO-303T-A2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-D1規(guī)格:LVSO-303T-D1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-D2規(guī)格:LVSO-303T-D2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-WD2規(guī)格:LVSO-303T-WD2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-D1TB規(guī)格:LVSO-303T-D1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-D2TB規(guī)格:LVSO-303T-D2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-A1規(guī)格:LVSO-303G-A1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-A2規(guī)格:LVSO-303G-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-A1TB規(guī)格:LVSO-303G-A1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-A2TB規(guī)格:LVSO-303G-A2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-D1規(guī)格:LVSO-303G-D1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-D2規(guī)格:LVSO-303G-D2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-D2TB規(guī)格:LVSO-303G-D2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-A1規(guī)格:LVSO-304G-A1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-A2規(guī)格:LVSO-304G-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-A1TB規(guī)格:LVSO-304G-A1TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-A2TB規(guī)格:LVSO-304G-A2TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-D1規(guī)格:LVSO-304G-D1品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-D2規(guī)格:LVSO-304G-D2品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-D2-TB-F規(guī)格:LVSO-304G-D2-TB-F品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-304G-D1TB規(guī)格:LVSO-304G-D1TB品牌:FUJI ENGINEERING產(chǎn)品名:SMD-4A-24-ML規(guī)格:SMD-4A-24-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-202T規(guī)格:LVMH-202T品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-202T-NO規(guī)格:LVMH-202T-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-203T規(guī)格:LVMH-203T品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-203T-NO規(guī)格:LVMH-203T-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-204T規(guī)格:LVMH-204T品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-204T-NO規(guī)格:LVMH-204T-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-202G規(guī)格:LVMH-202G品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-202G-NO規(guī)格:LVMH-202G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-203G規(guī)格:LVMH-203G品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-203G-NO規(guī)格:LVMH-203G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-204G規(guī)格:LVMH-204G品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-204G-NO規(guī)格:LVMH-204G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-206G規(guī)格:LVMH-206G品牌:FUJI ENGINEERING產(chǎn)品名:LVMH-206G-NO規(guī)格:LVMH-206G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-203T規(guī)格:LVPH-203T品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-204T規(guī)格:LVPH-204T品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-203G規(guī)格:LVPH-203G品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-203T-NO規(guī)格:LVPH-203T-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-204T-NO規(guī)格:LVPH-204T-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-203G-NO規(guī)格:LVPH-203G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-204G-NO規(guī)格:LVPH-204G-NO品牌:FUJI ENGINEERING產(chǎn)品名:LVPH-204G-NO規(guī)格:LVPH-204G-NO品牌:FUJI ENGINEERING產(chǎn)品名:AS-03T防爆閥規(guī)格:AS-03T品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-A1D-TB規(guī)格:LVSH-204G-A1D-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-204G-WA1D-W規(guī)格:LVSH-204G-WA1D-W品牌:FUJI ENGINEERING產(chǎn)品名:LVS2-03T-A2-TB規(guī)格:LVS2-03T-A2-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-203T-A1-TB-ML規(guī)格:LVSH-203T-A1-TB-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVS2-03T-A1-TB規(guī)格:LVS2-03T-A1-TB品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303G-WD2X規(guī)格:LVSO-303G-WD2X品牌:FUJI ENGINEERING產(chǎn)品名:EM-04T防爆閥規(guī)格:EM-04T品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-203G-A2-TB-ML規(guī)格:LVSH-203G-A2-TB-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVSH-203T-A1-TB-ML規(guī)格:LVSH-203T-A1-TB-ML品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-306G-A2規(guī)格:LVSO-306G-A2品牌:FUJI ENGINEERING產(chǎn)品名:LVS2-202T-WA2規(guī)格:LVS2-202T-WA2品牌:FUJI ENGINEERING產(chǎn)品名:EM-04G防爆閥規(guī)格:EM-04G品牌:FUJI ENGINEERING產(chǎn)品名:EM-04F防爆閥規(guī)格:EM-04F品牌:FUJI ENGINEERING產(chǎn)品名:EM-06T防爆閥規(guī)格:EM-06T品牌:FUJI ENGINEERING產(chǎn)品名:EM-06G防爆閥規(guī)格:EM-06G品牌:FUJI ENGINEERING產(chǎn)品名:EM-06F防爆閥規(guī)格:EM-06F品牌:FUJI ENGINEERING產(chǎn)品名:EM-06F防爆閥規(guī)格:EM-06F品牌:FUJI ENGINEERING產(chǎn)品名:LVSO-303T-A2-KK規(guī)格:LVSO-303T-A2-KK品牌:FUJI ENGINEERING
電子精密天平功能: ·去皮重·累計(jì)稱量·故障報(bào)警·懸掛稱量·自動(dòng)校正·積分時(shí)間可調(diào)·靈敏度可調(diào)·稱量單位轉(zhuǎn)換(克、克拉、盎司)·計(jì)數(shù)功能·打印功能·RS232C,標(biāo)準(zhǔn)輸出接口 電子精密天平型號(hào) JA1003 JA1203 JA2003 JA3003 JA5003稱量范圍 0~100g 0~120g 0~200g 0~300g 0~500g讀數(shù)精度 1mg稱盤(pán)尺寸 Φ80mm輸出接口 RS232C外型尺寸 324mm*217mm*335mm凈重 7kg電源 220V/50Hz 110V/60Hz
中文名稱:大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒英文名稱: Rat c-Jun N-terminal kinases,JNK ELISA Kit
購(gòu)買我公司任一款elisa試劑盒,均可享受免費(fèi)代測(cè)服務(wù)。
大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒 實(shí)驗(yàn)材料與試劑配制:1.儀器與材料:酶標(biāo)儀(使用前預(yù)熱30分鐘),微量加液器、吸頭、蒸餾水或去離子水,濾紙。2.緩沖液使用:加5ul的緩沖液于50ul的樣品中,如果樣品量不夠或者不確定,緩沖液和樣品的混合比例不要小于1:10即可。 混勻,靜置1小時(shí)備用(如果標(biāo)本是血清或者血漿,此步驟忽略)。3.洗液的配制:按1:100的比例配制洗液備用。大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒樣品收集、處理及保存1.細(xì)胞培養(yǎng)上清:適用于檢測(cè)體外培養(yǎng)的細(xì)胞分泌性成份。用無(wú)菌管收集細(xì)胞上清液,以1000×g離心15分鐘,收集上清。2.細(xì)胞:用PBS反復(fù)洗滌細(xì)胞3次,調(diào)整細(xì)胞濃度達(dá)到104-106/ml 左右,通過(guò)反復(fù)凍融,使細(xì)胞破壞并放出細(xì)胞內(nèi)成份,或者細(xì)胞超聲粉碎,離心取上清液檢測(cè)。3.血清:在室溫下,血液自然凝固,以1000×g離心15分鐘,取上清待測(cè)。|4.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA 或檸檬酸鈉作為抗凝劑,混合后靜置10-20 分鐘后,以1000×g離心15分鐘,收集上 清。5.體液:包括胸腹水、腦脊液,分泌物等。使用不含熱原和內(nèi)毒素的離心管收集,以1000×g離心15分鐘,收集上清。6.組織標(biāo)本:切取組織標(biāo)本,稱取重量0.5mg,加入500ul的PBS,用手工或勻漿器,或超聲破碎儀將標(biāo)本勻漿,以2000-3000 rmp離心20 分鐘,收集上清進(jìn)行檢測(cè)。7.樣品中不能含有NaN3,因NaN3 抑制辣根過(guò)氧化物酶的(HRP)活性。8.保存:如果樣品不能立即檢測(cè),應(yīng)將其分裝,-70 ℃保存,避免反復(fù)冷凍。保存過(guò)程中如出現(xiàn)沉淀,應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒,檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒 操作步驟:1.取出試劑盒,室溫(20-25℃)放置 30分鐘。2.分組:取出 96孔板,根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù),把剩余的板條繼續(xù)冷藏處理。分別設(shè)標(biāo)準(zhǔn) 品組(6個(gè)濃度)、空白孔、待測(cè)樣品組。注:為減少實(shí)驗(yàn)誤差,保證準(zhǔn)確性,建議設(shè)置復(fù)孔。3.加樣:依照標(biāo)準(zhǔn)品的順序分別加入 100ul的標(biāo)準(zhǔn)品溶液于空白微孔中;空白對(duì)照孔加入 100ul的蒸餾水;其余微孔中加入100ul的待測(cè)樣本。4.加酶標(biāo)溶液:標(biāo)準(zhǔn)品組、待測(cè)樣本組各孔中加入 50ul的酶標(biāo)溶液(空白對(duì)照孔除外)。5.溫育:酶標(biāo)板用封板紙密封后,放入濕盒內(nèi)于 37℃恒溫孵育1小時(shí)。6.洗板:用稀釋后的洗滌液注滿每孔,靜置15-30s,充分清洗酶標(biāo)板 5次,用吸水紙徹底拍干。7.顯色:各孔加入顯色劑A液50ul后,再加入顯色劑B液50ul。8.終止:25-37℃下避光反應(yīng)10-15分鐘,加入50ul終止液。9.讀板:在450nm波長(zhǎng)讀取各孔的OD 值。 注:讀板時(shí)必須拭干板底殘留的液體和手指痕跡,讀板時(shí)間控制在終止反應(yīng)后的 30分鐘內(nèi),以免影響準(zhǔn)確性。大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒 注意事項(xiàng):1.實(shí)驗(yàn)操作中必須使用一次性吸頭,避免交叉污染。2.加樣:加樣時(shí),要控制加樣速度,避免第一孔與最后一孔間的時(shí)間間隔過(guò)大,否則將會(huì)導(dǎo)致不同的預(yù)孵育時(shí)間,從而影響實(shí)驗(yàn)的準(zhǔn)確性以及重復(fù)性。3.孵育:嚴(yán)格按照說(shuō)明書(shū)上規(guī)定的孵育時(shí)間和溫度進(jìn)行。4.反應(yīng)時(shí)間的控制:加入底物后請(qǐng)定時(shí)觀察反應(yīng)孔的顏色變化,如果顏色較深,請(qǐng)?zhí)崆凹尤虢K止液終止反應(yīng)。5.建議實(shí)驗(yàn)前預(yù)測(cè)樣品含量,如樣品濃度過(guò)高,應(yīng)對(duì)樣品進(jìn)行稀釋,計(jì)算結(jié)果時(shí)乘以相應(yīng)的稀釋倍數(shù)。6.建議使用本試劑盒時(shí)先做預(yù)實(shí)驗(yàn)(即先做標(biāo)準(zhǔn)曲線,試用幾個(gè)標(biāo)本),如果對(duì)本試劑盒有任何疑問(wèn),可和所購(gòu)經(jīng)銷商聯(lián)系,如果因運(yùn)輸過(guò)程導(dǎo)致試劑盒失效,可要求調(diào)換,但概不承擔(dān)產(chǎn)品本身以外的任何損失。大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒安全性1. 避免人體直接接觸顯色劑A、B和終止液。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。2. 實(shí)驗(yàn)中不要進(jìn)食、抽煙或使用化妝品。3. 不要用嘴吸取試劑盒里的任何成份。大鼠c-Jun氨基末端激酶(JNK)elisa試劑盒 保存條件及有效期1.試劑盒保存:2-8℃2.有效期:6個(gè)月
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Japan 分裝 Cellulase R-10 纖維素酶R-10價(jià)格
Cellulase R-10纖維素酶R-10
名稱:1,4-β-D-葡聚糖葡糖苷水解酶;Cellulase Onozuke R-10
CAS#:9012-54-8
性狀:淡黃色或白色粉末
儲(chǔ)存條件:2~8℃ From Japan YakultCellulase R-10纖維素酶R-10名稱:1,4-β-D-葡聚糖葡糖苷水解酶;Cellulase Onozuke R-10CAS#:9012-54-8性狀:淡黃色或白色粉末儲(chǔ)存條件:2~8℃ From Japan Yakult
雜質(zhì)的去除:
酶提取液中常含有雜蛋白、多糖、脂類及核酸等雜質(zhì),可用下述方法去除: 調(diào)PH和加熱法:利用蛋白質(zhì)對(duì)酸、堿和熱變性方面性質(zhì)的差異,可去除非活性雜蛋白。如制備脂肪酶時(shí),在pH 3、4時(shí)以400C溫度加熱150 min,淀粉酶活力可喪失90%而被除去,而脂肪酶活力仍保持80%以上。 蛋白質(zhì)表面變性法:蛋白質(zhì)表面變性后其性質(zhì)有所不同,借以去除雜蛋白。如制備過(guò)氧化氫酶時(shí),加入氯仿和乙醇進(jìn)行振蕩可以將雜蛋白變性而去除。 蛋白質(zhì)沉淀劑法:利用醋酸鋁、利凡諾、單寧酸、離子型表 面活性劑等蛋白質(zhì)沉淀劑可以去除雜蛋白及粘多糖類雜質(zhì)。使用時(shí)要注意這類試劑??梢鹈缸冃允Щ?,因此應(yīng)迅速除去
全國(guó)咨詢熱線:010-56429247 13488645491 QQ:774226171 聯(lián)系人:梁經(jīng)理
型號(hào):MJX-0450
容積:450L
外形尺寸:630×800×1950
工作室尺寸:510×580×1341
控溫范圍、精度:-10~50℃,±0.1℃
控濕范圍、精度:50~95%RH,±1%RH
溫度波動(dòng)度:±1℃\±5%RH
其他參數(shù):紫外線滅菌,環(huán)保制冷可選配裝置:多端編程,計(jì)算機(jī)控制,遠(yuǎn)程無(wú)線報(bào)警,聲光報(bào)警,超欠溫保護(hù),定時(shí)換氣 霉菌培養(yǎng)箱MJX-0450技術(shù)參數(shù):
型號(hào):MJX-0450
容積:450L
外形尺寸:630×800×1950
工作室尺寸:510×580×1341
控溫范圍、精度:-10~50℃,±0.1℃
控濕范圍、精度:50~95%RH,±1%RH
溫度波動(dòng)度:±1℃\±5%RH
其他參數(shù):紫外線滅菌,環(huán)保制冷可選配裝置:多端編程,計(jì)算機(jī)控制,遠(yuǎn)程無(wú)線報(bào)警,聲光報(bào)警,超欠溫保護(hù),定時(shí)換氣
概述: ZJHP(ZJHM)調(diào)節(jié)閥是具有當(dāng)今國(guó)際先進(jìn)水平的最新一代氣動(dòng)直通精小型調(diào)節(jié)閥,它采用新型多彈簧氣動(dòng)薄膜執(zhí)行機(jī)構(gòu)與低流阻直通閥組合成為單座閥和套筒閥兩種,具有相當(dāng)大的流量系數(shù)和精確的流量特性。精細(xì)加工的推桿導(dǎo)向表面,硬度和光潔度高,增加了密封性,減少了回差。調(diào)節(jié)閥符合IEc國(guó)際標(biāo)準(zhǔn),集結(jié)構(gòu)緊湊,動(dòng)作穩(wěn)定,可調(diào)比大和輕便小巧等特點(diǎn)于一體,廣泛應(yīng)用于化工、冶金、煉油和電站等工業(yè)自動(dòng)控制系統(tǒng)中。
性能規(guī)范:
J21W/H內(nèi)外螺紋壓力表針型閥設(shè)計(jì)制造:GB/T12224-1989,JB/T7747-1995,連接形式:內(nèi)外螺紋連接,結(jié)構(gòu)特征:錐螺紋密封;活動(dòng)閥瓣,閥門(mén)檢驗(yàn)和試驗(yàn):GB/T13927-1992。
試驗(yàn)項(xiàng)目 TESTITEM | 壓力值 PRESSURE VALVE |
公稱壓力 Nominal Pressure | 32 |
殼體試驗(yàn) Shell Test | 48 |
密封試驗(yàn) Test | 35.2 |
氣壓試驗(yàn) Air Pressure Test | 19.2 |
適用溫度 Suitable Temperatue | ≤550℃ |
適用介質(zhì) Suitable Medium | 水、蒸汽、油品等Water,Steam,Oilgoods |
型號(hào) TYPE | 閥體 BODY | 接頭 TIE-IN | 閥桿 VALVE HANDLE | 螺帽 SCREW CAP | 填料 PACKING |
J61H-160 | 20# | 20# | 2Cr13 | 20# | 柔性石墨、四氟 Leslble graphite |
J61W-160P | 1Cr18Ni9Ti | 1Cr18Ni9Ti | 1Cr18Ni9Ti | 1Cr18Ni9Ti | 柔性石墨、四氟 Leslble graphite |
J61Y-160V | 12CrMov | 12CrMov | 12CrMov | 20# | 柔性石墨、四氟 Leslble graphite |
規(guī)格 SPECIF ICATION | Do | D | H | L |
DN6 | Φ14 | Φ70 | 74 | 60 |
DN10 | Φ18 | Φ80 | 85 | 60 |
DN15 | Φ22 | Φ80 | 102 | 65 |