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 儀器介紹

◆采用一只蓋革-彌勒計(jì)數(shù)管來測(cè)定α、β、γ和X射線輻射  ◆“安全第一”（Safety First）的校準(zhǔn)功能能夠避免校準(zhǔn)人員的輻射接觸  ◆檢測(cè)儀符合歐洲CE認(rèn)證要求 

主要特點(diǎn)

◆內(nèi)置鹵素淬滅劑GM探測(cè)器，對(duì)α、β射線源的靈敏度很高 ◆四位液晶顯示，可選擇mR/hr、CPM、mSv/hr、CPS或Total/Timer等單位 ◆總計(jì)數(shù)/定時(shí)器功能對(duì)輕微污染進(jìn)行定時(shí)的精確檢測(cè)，定時(shí)時(shí)間可選擇1分鐘－－    24小時(shí)

技術(shù)參數(shù) 

◆測(cè)量范圍：mR/hr(毫倫/小時(shí))：0.001—110.0，CPM（每分鐘計(jì)數(shù)）：0—300,000    μSv/hr(微希伏/小時(shí))：0.01—1,100，CPS（每秒鐘計(jì)數(shù)）：0—5,000，總計(jì)數(shù)：       1—9,999,000 ◆效 率：Sr-90(546kev,2.3MeV βmax)約75%  C-14(156kev βmax)約11%    Bi-210(1.2MeV βmax)約64%  Am-241(5.5MeV α)約36% ◆靈 敏 度：3500CPM/ mR/hr（對(duì)于Cs-137） ◆精 度：±15% ◆溫度范圍：-10℃－－-+50℃ ◆電 源：1節(jié)9V堿性電池，電池壽命 200小時(shí)◆尺寸重量：150×80×30mm 350克（含電池）

應(yīng)用領(lǐng)域

◆探測(cè)和測(cè)定表面沾污◆在操作放射性核素時(shí)監(jiān)測(cè)可能存在的放射性暴露量◆調(diào)查環(huán)境污染◆測(cè)定惰性氣體及其它低能放射性核素◆建筑裝飾材料放射測(cè)定 射線危害：低劑量的放射性射線輻射（天然背景輻射的變化范圍），對(duì)人體無(wú)害或風(fēng)險(xiǎn)甚低，但達(dá)到一定劑量則會(huì)對(duì)人體有害，可引起癌癥、白內(nèi)障、不孕癥、突變、萎縮效應(yīng)、壽命減短,甚至死亡

應(yīng)用：

偵測(cè)放射性射線，以采取相應(yīng)防護(hù)措施。海關(guān)和邊境巡邏，政府執(zhí)法部門，檢疫檢驗(yàn)，應(yīng)急事故處理，核電廠、銀行、政府、實(shí)驗(yàn)室等部門安全巡查，醫(yī)學(xué)廢料處理，消防隊(duì)，采礦業(yè)，科學(xué)實(shí)驗(yàn)，個(gè)人保護(hù)，連續(xù)監(jiān)測(cè)

參考信息（來自中國(guó)輻射防護(hù)研究院）

居民的劑量限值為每年1mSv。即0.114μSv/hr。

放射性職業(yè)人員劑量限值為每年20mSv，但任何一年不能超過50mSv。

美國(guó)米頓羅GM系列機(jī)械隔膜式計(jì)量泵/GM0120米頓羅加藥泵/蘇州加藥泵

計(jì)量泵GM0050 /米頓羅GM0050/米頓羅計(jì)量泵GM0050/蘇州GM0050計(jì)量泵

GM0090米頓羅/GM0090計(jì)量泵/米頓羅計(jì)量泵GM0090/蘇州GM0090計(jì)量泵

米頓羅GM0120 /計(jì)量泵GM0120 /米頓羅計(jì)量泵GM0120/蘇州GM0120計(jì)量泵

米頓羅GM0170 /計(jì)量泵GM0170 /米頓羅計(jì)量泵GM0170/蘇州GM0170計(jì)量泵

GM0500米頓羅/GM0500計(jì)量泵/米頓羅計(jì)量泵GM0500/蘇州GM0500計(jì)量泵

主要型號(hào)包括：GM0050 GM0090 GM0120 GM0170 GM0240 GM0330 GM0400 GM0500

特點(diǎn)：

平滑脈沖設(shè)計(jì)，無(wú)沖擊 ? 沖程在靜態(tài)及動(dòng)態(tài)條件下均可調(diào)節(jié)

多種材質(zhì)及泵頭結(jié)構(gòu)可選 ? 可選配電動(dòng)沖程控制器

機(jī)械驅(qū)動(dòng)PTFE膜片 ? 最大液體溫度40 ℃＊

手動(dòng)/ 自動(dòng)沖程調(diào)節(jié)可選 ? 穩(wěn)態(tài)精度 +/- 2%(10%~100%)

沖程可調(diào)0~100%(自動(dòng)/手動(dòng)) ? 最大吸程4m水柱＊

可選配電機(jī)控制器實(shí)現(xiàn)外部信號(hào)自動(dòng) 控制， ? 最大吸入壓力：20m 水柱

電機(jī)頻率0~100%可調(diào)(GM泵 自動(dòng)控制方式)

米頓羅機(jī)械隔膜泵，GM和GB系列兩個(gè)系列。泵頭材質(zhì)為PVC，PP，SS316，高粘度等可選擇，適合多種場(chǎng)合液體介質(zhì)的輸送。流量范圍從2.25L到1800L均有。

可選雙隔膜泵頭結(jié)構(gòu)

建議維護(hù)周期： 自動(dòng)控制方式選擇

電動(dòng)沖程控制器接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程長(zhǎng)度， 從而改變計(jì)量泵輸出流量。

GM系列計(jì)量泵選擇ST型。 GB系列計(jì)量泵選擇ECC型。

變頻控制器：接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程頻率，從而改變計(jì)量泵輸出流量。

供 電：220V/50HZ 單向

380V/50HZ 三相 控制信號(hào)：4~20mA 電機(jī)控制器(VARIPULSE):

僅用于GM系列計(jì)量泵，用于控制三相電機(jī)，改變沖程頻率，從而調(diào)節(jié)計(jì)量泵輸出流量。

V 型－同計(jì)量泵一體式安裝   VR型－同計(jì)量泵分體式安裝

主要型號(hào)包括：GM0050 GM0090 GM0120 GM0170 GM0240 GM0330 GM0400 GM0500

上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

蘇州米頓羅機(jī)械隔膜計(jì)量泵

上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

特點(diǎn)：

•平滑脈沖設(shè)計(jì)，無(wú)沖擊 •沖程在靜態(tài)及動(dòng)態(tài)條件下均可調(diào)節(jié)

•多種材質(zhì)及泵頭結(jié)構(gòu)可選 •可選配電動(dòng)沖程控制器

•機(jī)械驅(qū)動(dòng)PTFE膜片 •最大液體溫度40 ℃＊

•手動(dòng)/ 自動(dòng)沖程調(diào)節(jié)可選 •穩(wěn)態(tài)精度±2%(10%~100%)

•沖程可調(diào)0~100%(自動(dòng)/手動(dòng)) •最大吸程4m水柱＊

•可選配電機(jī)控制器實(shí)現(xiàn)外部信號(hào)自動(dòng) 控制，•最大吸入壓力：20m 水柱

電機(jī)頻率0~100%可調(diào)(GM泵 自動(dòng)控制方式)

•可選雙隔膜泵頭結(jié)構(gòu)

•建議維護(hù)周期：≤1.0MPa 4000h 　　　　　　

≤0.7MPa 8000h

自動(dòng)控制方式選擇

•電動(dòng)沖程控制器 接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程長(zhǎng)度， 從而改變計(jì)量泵輸出流量。

GM系列計(jì)量泵選擇ST型。 GB系列計(jì)量泵選擇ECC型。

•變頻控制器： 接受外部控制信號(hào)，調(diào)節(jié)計(jì)量泵沖程頻率，從而改變計(jì)量泵輸出流量。

供 電：220V/50HZ 單向

380V/50HZ 三相

控制信號(hào)：4~20mA

•電機(jī)控制器(VARIPULSE):

僅用于GM系列計(jì)量泵，用于控制三相電機(jī)，改變沖程頻率，從而調(diào)節(jié)計(jì)量泵輸出流量。

V 型－同計(jì)量泵一體式安裝

VR型－同計(jì)量泵分體式安裝

1Human cyclic adenosinemonophosphate（cAMP）Elisa KitCatalog No. CSB-E04488h（96T） This immunoassay kit allows for the in vitro quantitative determination of humancAMP concentrations in serum, plasma. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2INTRODUCTIONCyclic adenosine monophosphate （cAMP, cyclic AMP or 3＇-5＇-cyclicadenosine monophosphate） is a second messenger important in manybiological processes. cAMP is derived from adenosine triphosphate （ATP）and used for intracellular signal transduction in many different organisms,conveying the cAMP-dependent pathway.cAMP is synthesised from ATP by adenylyl cyclase located on the innerside of the plasma membrane. Adenylyl cyclase is activated by a range ofsignaling molecules through the activation of adenylyl cyclase stimulatory G（Gs）-protein-coupled receptors ａnd inhibited by agonists of adenylylcyclase inhibitory G （Gi）-protein-coupled receptors. Liver adenylyl cyclaseresponds more strongly to glucagon, ａnd muscle adenylyl cyclase respondsmore strongly to adrenaline.cAMP decomposition into AMP is catalyzed by the enzymephosphodiesterase.cAMP is a second messenger, used for intracellular signal transduction,such as transferring the effects of hormones like glucagon ａnd adrenaline,which cannot pass through the cell membrane. It is involved in theactivation of protein kinases ａnd regulates the effects of adrenaline andglucagon. It also regulates the passage of Ca2+ through ion channels.3PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated with agoat-anti-mouse IgG. Standards or samples are then added to theappropriate microtiter plate wells with a HRP-conjugated cAMP andantibody preparation specific for cAMP ａnd incubated. Then substratesolutions are added to each well. The enzyme-substrate reaction isterminated by the addition of a sulphuric acid solution ａnd the color changeis measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Theconcentration of cAMP in the samples is then determined by comparing theO.D. of the samples to the standard curve.DETECTION RANGE0.08pmol/ml-250 pmol/ml. The standard curve concentrations used for theELISA’s were 250 pmol/ml, 50 pmol/ml, 10 pmol/ml, 2 pmol/ml, 0.4 pmol/ml,0.08 pmol/ml.SPECIFICITYThis assay recognizes human cAMP No significant cross-reactivity orinterference was observed.4MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard1 x 0.25 ml（5000pmol/ml）HRP-conjugate 1（1000 x stock solution）Antibody 1（1000 x stock solution）HRP-conjugate Diluent 1 x 6 mlAntibody Diluent 1 x 6 mlNeutralizing Reagent 1 x 6 mlWash Buffer1 x 15 ml（10×concentrate）Substrate A 1 x 10 mlSubstrate B 1 x 10 mlStop Solution 1 x 6 mlOTHER SUPPLIES REQUIRED1. Deionized or distilled water.2. Concentrated HCl.3. Precision Pipets for volumes between 5μ l ａnd 1000μ l.4. Repeater Pipets for dispensing 50μ l ａnd 200μ l.5. Disposable breakers for diluting buffer concentrates.6. Graduated cylinders.7. A microplate shaker.8. Adsorbent paper for blotting.9. Microplate reader capable of reading at 450 nm, preferably withcorrection between 570 ａnd 590 nm.5STORAGE1. For long-term best results, store stocks of the Standard ,Antibody andHRP-conjugate at -80℃. All other components of this kit are stable at4°C until the kit＇s expiration date.SAMPLE COLLECTION AND STORAGEThis ELISA is compatible with cAMP samples that have been treated withhydrochloric acid to stop endogenous phosphodiesterase activity. Samplesin this matrix can be measured directly without evaporation or furthertreatment.Tissue samples should be frozen in liquid nitrogen. The tissue should beground to a fine powder under liquid nitrogen in a stainless steel mortar.After the liquid nitrogen has evaporated, weigh the frozen tissue andhomogenize in 10 volumes of 0.1M HCl. Centrifuge at > 600 x g at roomtemperature. The samples can then be diluted in the 0.1M HCl.Cells grown in tissue culture media can be treated with 0.1M HCl after firstremoving the media. Incubate for 10 minutes ａnd visually inspect the cellsto verify cell lysis. If adequate lysis has not occurred incubate for a further10 minutes ａnd inspect. Centrifuge at 600 x g at room temperature, thenuse the supernatant directly in the assay. Cell or tissue lysis can beenhanced by adding 0.1% to 1% Triton x-100 to the 0.1M HCl prior to use.When used in this concentration range, the detergent will not interfere with6the binding portion of the assay, however there will be a modest increase inthe optical density. Samples containing Triton should be evaluated against astandard curve diluted in the same for the most accurate determination.Cyclic AMP in the media can be measured after treating 1 mL of thesupernatant media with 10 μ L of concentrated hydrochloric acid.Centrifuge at 600 x g at room temperature. The supernatants can then beused directly in the assay.Procedural Notes1. Allow all reagents to warm to room temperature for at least 30 minutesbefore opening.2. Pre-rinse the pipet tip with reagent ,use fresh pipet tips for eachsample,standard ａnd reagent.3. Pipet standards ａnd samples to the bottom of the wells.4. Add the reagents to the side of the well to avoid contamination.5. The kit uses break-apart microtiter strips,which allow the user tomeasure as many samples as desired.Unused wells must be keptdesiccated at 4℃ in the sealed bag provided,The wells should be usedin the frame provided.6. Prior to addition of substrate ensure that there is no residual washbuffer in the wells .Any remaining wash buffer may cause variationin assay resultes.REAGENT PREPARATIONBring all reagents to room temperature before use.71. Wash Buffer If crystals have formed in the concentrate, warm to roomtemperature ａnd mix gently until the crystals have completely dissolved.Dilute 15 ml of Wash Buffer Concentrate into deionized to prepare 150ml of Wash Buffer.2. HRP-conjugate working solution: Centrifuge the vial right before use.Dilute the HRP-conjugate 1000x stock solution （take 6μl） with theprovided dilution buffers （6 ml）.3. Antibody working solution: Centrifuge the vial right before use. Dilutethe Antibody 1000x stock solution （take 6μl） with the provided dilutionbuffers （6 ml）.4. Standards: Centrifuge the vial right before use. Allow the 5,000pmol/mL cAMP standard solution to warm to room temperature. Labelsix tubes #1 through #6. Pipet 475 μL 0.1M HCl into tube #1 ａnd 400 μL0.1M HCl into tubes #2-6. Add 25 μL of the 5,000 pmol/mL standard totube #1. Vortex thoroughly. Add 100 μL of tube #1 to tube #2 andvortex thoroughly. Continue this for tubes #3 through #6. Theconcentration of cAMP in tubes #1 through #6 will be 250, 50, 10, 2, 0.4,and 0.08 pmol/mL respectively. Diluted standards should be usedwithin 30 minutes of preparation. Label one tube as the ZeroStandard/NSB tube. Pipet 600μl 0.1M HCl into this tube.ASSAY PROCEDUREBring all reagents to room temperature for at least 30 minutes prioropening.ALL standards ａnd samples should be run in duplicate.Add the reagen directly to the samples ａnd vortex for 2 seconds immediatelyafter the addition.81. Refer to the Assay Layout Sheet to determine the number of wells to beused ａnd put any remaining wells with the desiccant back into thepouch ａnd seal the ziploc .Store unused wells at 4℃.2. Pipet 50 μL of the Neutralizing Reagent into each well, except theTA（Total Activity） ａnd Blank wells.3. Pipet 100 μL of 0.1M HCl into the NSB（None Specific Binding） ａnd theBo （0 pmol/mL Standard） wells.4. Pipet 100 μL of Standards into the appropriate wells.5. Pipet 100 μL of the Samples into the appropriate wells.6. Pipet 50 μL of 0.1 M HCl into the NSB wells.7. Pipet 50 μL of HRP-conjugate working solution into each well exceptthe TA ａnd Blank wells.8. Pipet 50 μL of Antibody working solution into each well, except theBlank, TA ａnd NSB wells.9. Incubate the plate at room temperature for 2 hours on a plate shaker at250~500 rpm.10. Empty the contents of the wells ａnd wash by adding 400 μL of washsolution to every well. Repeat the wash 2 more times for a total of 3washes.11. After the final wash, empty or aspirate the wells, ａnd firmly tap the plateon a lint free paper towel to remove any remaining wash buffer.12. Add 5 μL of the HRP-conjugate working solution to the TA wells.913. Add 200 μL of the Substrate solution to every well. Incubate at roomtemperature for 5~30 minutes without shaking. A gradient of blue colorshould become visible during the incubation period. （Substrate A andB should be mixed together in equal volumes within 15 minutes ofuse. Protect from light.）14. Add 50 μL of Stop Solution to every well. This stops the reaction andthe plate should be read immediately.15. Blank the plate reader against the Blank wells, read the optical densityat 450 nm （for HRP）, preferably with correction between 570 ａnd 590nm. If the plate reader is not able to be blanked against the Blankwells, manually subtract the mean optical density of the Blank wellsfrom all readings.1 0CALCULATION OF RESULTSSeveral options are available for the calculation of the concentration ofcAMP in the samples. The X-axis is the concentration of cAMP for thestandards. The Y-axis is either the Average Net Optical Density or thePercent Bound.1. Calculate the average Net Optical Density （OD） bound for each standardand sample by subtracting the average NSB OD from the average ODbound:Average Net OD = Average Bound OD － Average NSB OD2. Calculate the binding of each pair of standard wells as a percentage ofthe maximum binding wells （Bo）, using the following formula:3. Using Logit-Log paper plot Average Net OD or Percent Bound（B/Bo） versus concentration of cAMP for the standards. Theconcentration of cAMP in the unknowns can be determined byinterpolation.1 1Typical Standard CurvesThese curves must not be used to calculate cAMP concentrations; eachuser must run a standard curve for each assay ａnd version used.SensitivitySensitivity was calculated by determining the average optical density boundfor ten wells run with the Bo, ａnd comparing to the average optical densityfor ten wells run with Standard #5. The detection limit was determined asthe concentration of cAMP measured at two standard deviations from thezero along the standard curve.Non-Acetylated VersionMean OD for Bo = 0.685±0.003Mean OD for Standard #5 = 0.604±0.010Delta Optical Density（0-0.08pmol/ml） = 0.0812 SD＇s of the Zero Standard = 0.006Sensitivity =￣0.006/0.081×0.4pmol/ml = 29.6 fmol/mL1 2LinearityA sample containing 16.0 pmol/mL cAMP was serially diluted 7 times 1:2 inthe 0.1M HCl ａnd measured. The data was plotted graphically as actualcAMP concentration versus measured cAMP concentration. The lineobtained had a slope of 1.000 with a correlation coefficient of 0.999.Cross ReactivitiesThe cross reactivities for a number of related compounds were determinedby competition ELISA assays. Potential cross reactants were dissolved inthe kit Assay Buffer at concentrations from 500,000 to 500 pmol/mL. Thesesampleswere then measured in the cAMP assay, ａnd the measured cAMPconcentration at 50% B/Bo calculated. The % cross reactivity wascalculated by comparison with the actual concentration of cross reactant inthe sample ａnd expressed as a percentage.Compound Cross ReactivitycAMP 100%AMP <0.0001%ATP <0.0001%cGMP <0.0001%GMP <0.0001%GTP <0.0001%cUMP <0.0001%CTP <0.0001%

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一、PXR型數(shù)字溫控表的功能特點(diǎn)如下：　　　　　1. 前面板IP66防水結(jié)構(gòu)，三健式菜單操作；2．標(biāo)準(zhǔn)螺釘接線，無(wú)須插座；3. 縱向尺寸比PXW表更短；4. UL/CSA/CE認(rèn)證標(biāo)志；5. 測(cè)量值大LED紅色顯示；6. 控制功能多種：簡(jiǎn)單ON/OFF控制，PID帶自動(dòng)調(diào)節(jié)控制，模糊及PID帶自動(dòng)調(diào)節(jié)控制，PID自適應(yīng)調(diào)節(jié)控制；7．再傳輸功能（選件）：傳感器測(cè)量值可以以4-20MA型式傳送到PHR型數(shù)據(jù)記錄儀，PLC及個(gè)人計(jì)算機(jī)中；8．8段斜坡/保溫程序控制功能（選件）；9．RS-485通訊功能（選件），可與FUJI POD及個(gè)人計(jì)算機(jī)通訊；10．?dāng)?shù)字輸入控制功能（選件）：通過一點(diǎn)開關(guān)量ON/OFF，可改變?cè)O(shè)定值SV，控制動(dòng)作起/停，斜坡/保溫控制的開始/ 復(fù)位，自動(dòng)調(diào)節(jié)功能的起/停，報(bào)警鎖存的復(fù)位、定時(shí)器計(jì)時(shí)開始；11. 加冷卻控制（選件）：有利于節(jié)能；12. 加熱斷線報(bào)警（選件）；13. 兩點(diǎn)各種報(bào)警功能（選件）：絕對(duì)值報(bào)警區(qū)間報(bào)警偏差報(bào)警;　14. 具有塑機(jī)專用的模糊+PID控制功能：15. 內(nèi)部定時(shí)器功能二、型號(hào)及尺寸PXR3 24×48×97 （高×寬×深）PXR5 96×48×78 （高×寬×深）PXR4 48×48×78.8 （高×寬×深）PXR9 96×96×79.5（高×寬×深）技術(shù)指標(biāo)控制類型: PID 控制、on/off控制、自整定、模糊控制單輸出或加熱/制冷雙