3G2A5-PS211
Item | Part Type | Description | Q'ty |
1 | C200H-OC222 | OUTPUT UNIT | 2 |
2 | C200H-IA122 | INPUT UNIT | 2 |
3 | C200H-ID212 | INPUT UNIT | 2 |
4 | C200H-OA222 | OUTPUT UNIT | 1 |
5 | C200H-LK401 | PC LINK UNIT | 1 |
6 | C200H-ASC02 | PSCII UNIT | 1 |
7 | 3G8B3-MA011 | MVME | 2 |
8 | 3G8B3-NO000 | MVME | 2 |
9 | NS-000V1 | OMEON CARD | 8 |
10 | G9SB-2002-A | SATETY RELAY UNIT 24VAC/DC | 30 |
11 | C500-ID218 | INPUT UNIT | 30 |
12 | C500-OC223 | OUTPUT UNIT | 6 |
13 | C500-OC224 | OUTPUT UNIT | 17 |
14 | C500-OC224 | OUTPUT UNIT | 1 |
15 | C1000HF-CPUA1-V1 | CPU UNIT | 5 |
16 | C1000HF-CPUA1-V1 | CPU UNIT | 1 |
17 | C500-PS221 | CPU Power UNIT | 9 |
18 | C500-PS221 | CPU Power UNIT | 7 |
19 | C4K-ID | INPUT UNIT | 2 |
20 | C60P-CDR-A | SYSMAC C60P PROGRAMMABLE CONTROLLER | 1 |
21 | C60P-CDR-A | SYSMAC C60P PROGRAMMABLE CONTROLLER | 2 |
22 | C4K-ID | SERVO MOTOR | 5 |
23 | R7M-A05030 | SERVO MOTOR 50W 200V 0.6A | 49 |
24 | R7M-A03030 | SERVO MOTOR 30W 200V 0.42A | 60 |
25 | R7M-AP10030 | SERVO MOTOR 100W 200V 0.89A | 87 |
26 | R7M-AP40030 | SERVO MOTOR 400W 200V 2.0A | 110 |
27 | R7M-AP75030 | SERVO MOTOR 750W 200V 4.1A | 30 |
28 | R88D-WT15H | AC SERVO DRIVER AC-INPUT:200-230V AC 50/60Hz 3PHASE AC-OUTPUT:0V-230V 3PHASE | 4 |
29 | R88D-WTA5HL | OUTPUT 0-300Hz | 2 |
30 | R88D-UT04V | AC SERVO DRIVER AC-INPUT:200-230V 50/60Hz 1PHASE AC-OUTPUT:0-230V 3PHASE | 1 |
31 | R88M-U05030TA-G33B | AC SERVO MOTOR 50W 200V 0.6A | 5 |
32 | R88M-U20030HA-S1 | AC SERVO MOTOR 200W 200V 2.0A | 3 |
33 | 3Y42A-H2710 | D10 | 1 |
34 | 3Y42A-H1320 | ROM | 2 |
35 | 3Y42A-H1210 | CAM | 1 |
36 | 3Y42A-H9721 | EXT | 1 |
37 | 3Y42A-H0401N | TIM | 1 |
38 | 3Y42A-H2750 | RELAY D0 100008E | 2 |
39 | 3Y42A-H2751 | RELAY D1 100007B | 2 |
40 | 3Y42A-H9620 | 200320B | 1 |
41 | 3Y42A-H9720 | 200324B | 1 |
42 | C20-CPU72 0620 | PROGRAMMABLE CONTROLLER | 1 |
43 | C200H-RM201 | REMOTE MASTER MODULE 2WIRE | 2 |
44 | C200H-CPU11-E | CPU UNIT | 1 |
45 | R88D-HT10 | AC SERVO DRIVER 200/220VAC 50/60Hz 65VA | 1 |
46 | R88D-HT04 | AC SERVO DRIVER 200/220VAC 50/60Hz 65VA | 5 |
47 | CVM1-CPU21-V2 | CPU UNIT CORPORATION | 1 |
48 | CVM1-PA208 | Power Supply Unit 100-12/200-240VAC 150VA 50/60Hz | 1 |
49 | C500-ID218 | Input Unit 24VDC.10mA | 3 |
50 | C500-OD412 | OUTPUT 24VDC RESISTIVE | 1 |
51 | C500-OA225 | Output Unit | 1 |
52 | CVM1-DRM21-V1 | COMPOBUS/D MASTER | 1 |
53 | C500-RM201 | REMOTE MASTER MODULE C500 | 2 |
54 | C500-MD211CN | IN/OUT (3G2A5-MD211) | 1 |
55 | F300-P | POWER SUPPLY UNIT | 3 |
56 | E5T-S91K | TEMPFRATURE CONTROLLER | 1 |
57 | CQM1-PA203 | Power Supply Unit | 6 |
58 | CQM1-OC221-1 | OUTPUT UNIT | 6 |
59 | CQM1-OD212 | Output Unit | 4 |
60 | CQM1-CPU11 | CPU Unit | 6 |
61 | G3PX-220EH | Power Controller | 5 |
62 | R88D-UA08HA | AC SERVO Driver | 2 |
63. | R88D-UA04HA | AC SERVO Driver 200/230VAC 100W 2.5A | 1 |
64 | R88D-WT150HF | AC SERVO Driver AC-INPUT:380-480V AC 3PHASE AC-OUTPUT:0V-480V 3PHASE | 2 |
65 | E3X-NT11 | OMRON PHOTOELECTRIC | 6 |
66 | E3X-H11 | PHOTOELECTRIC SWITCH | 1 |
67 | S82K-05024 | Power Supply | 3 |
68 | S82J-30024 | Power Supply | 4 |
69 | S82H-3024 | POWER SUPPLY 24VDC 2.5A | 1 |
70 | S82J-10024DD | POWER SUPPLY | 1 |
71 | S8PS-30024C | POWER SUPPLY | 1 |
72 | S8VS-01512 | POWER SUPPLY | 2 |
73 | S8VS-03005 | POWER SUPPLY | 2 |
74 | G3PB-215B-VD | Heater Control Relay | 2 |
75 | S82F-1524 | Power Supply 24 VDC | 1 |
76 | S82H-3024 | POWER SUPPLY 24VDC 2.5A | 1 |
77 | C200H-OD215 | Output Unit PLC | 1 |
78 | C200H-OD217 | OUTPUT MODULE 24VDC 12 POINT 0.3A | 1 |
79 | C500-ID212-IH | INPUT UNIT | 6 |
80 | C500-PS221 | CPU POWER UNIT | 3 |
81 | G7TC-ID16-5 | Position 24V Relay Unit | 1 |
82 | C500-RT002-V1 | INPUT UNIT | 1 |
83 | C200H-ID501 | OUTPUT UNIT 5VDC | 18 |
84 | C200H-MR831 | Memory Unit | 18 |
85 | C200HW-COM03-V1 | POWER SUPPLY UNIT | 14 |
86 | C200H-ID215 | Input Unit | 2 |
87 | C200H-OD215 | Output Unit | 2 |
88 | C200H-CPU02 | CPU Unit | 1 |
89 | C200H-OD219 | Output Unit | 9 |
90 | C200H-ID217 | Input Unit | 1 |
91 | C200HS-CPU01 | CPU Unit | 4 |
92 | C200H-OC224 | Output Unit | 18 |
93 | C200H-SP001 | Space Unit | 18 |
94 | CS1W-OD261 | Output Unit | 14 |
95 | C200H-OD211 | Output Unit | 1 |
96 | C200H-PS221 | Power Supply Unit | 1 |
97 | C200H-ME431 | MEMORY MODULE 4K EEPROM | 7 |
98 | C500-ID218 | INPUT UNIT | 149 |
99 | C500-OC223 | OUTPUT UNIT | 14 |
100 | C500-OC224 | OUTPUT UNIT | 55 |
101 | C1000HF-CPUA1-V1 | CPU UNIT | 29 |
102 | C500-PS221 | Power | 32 |
103 | C500-OD412 | OUTPUT 24VDC RESISTIVE | 4 |
104 | C1000H-CPU01-V1 | CPU unit | 1 |
105 | C500-PS223 | POWER SUPPLY MODULE CPU POWER UNIT 100-120/200-240VDC | 1 |
106 | 3G8B2-NI020 | PCB | 4 |
107 | 3G8B2-BI010 | PCB | 2 |
108 | 3G8B2-BI011 | PCB | 2 |
109 | 3G8B2-NO020 | PCB DV12V-24V 10mA | 4 |
120 | 3G8B2-CS000 | PCB | 2 |
121 | 3G8B3-MA010 | PCB | 2 |
122 | C200H-OD501 | 數(shù)位輸出模組 | 1 |
123 | C200H-ID215 | 數(shù)位輸入模組 | 4 |
124 | C200H-RM001-PV1 | 數(shù)位傳送模組 | 1 |
125 | C500-OC224 / 3G2A5-OC224 | CONNECTION DIAGRAM OUTPUT UNIT | 9 |
126 | C500-PS211 / 3G2A5-PS211 | CPU POWER UNIT | 1 |
127 | V600 | IDENTIFICATION SYSTEM CONTROLLER | 3 |
128 | R88-W10030H-R10 | AC SERVO MOTOR 100W 200V 0.91A | 4 |
129 | BX50XF(POWLI) | BX50XF | 1 |
130 | G3PA-220B | 100/110VAC 200/220VAC 20A | 2 |
131 | G32A-B | AC INPUT UNIT 200-240VAC OUTPUT 12VDC | 1 |
132 | G3NA-210B | SOLID STATE RELAY 240VAC 10A | 7 |
133 | S8VS-09024 | Digital Power Supply 24VDC/3.75A | 4 |
134 | S8VM-05015CD | POWER SUPPLY | 8 |
135 | S8VM-05024CD | POWER SUPPLY | 8 |
136 | C500-PRW06 | P-ROM WRITER | 1 |
137 | C500-AP003 | PRO-CON ADAPTER | 1 |
138 | C200H-BP001 | PRO-CON BASE | 1 |
139 | CQM1-PRO01 | PROGRAMMING CONSOLE UNIT | 1 |
140 | CVM1-PRO01 | PROGRAMMING CONSOLE UNIT | 1 |
141 | C500F-PRO19 | PROGRAMMING CONSOLE | 1 |
142 | C500-PS211 | CPU POWER UNIT | 1 |
143 | C1000HF-CPUA1-V1 | CPU UNIT | 6 |
144 | C500-PS211 | CPU POWER UNIT | 1 |
145 | C500-PS221 | CPU POWER UNIT | 3 |
146 | C500-OC223 | OUTPUT UNIT | 1 |
147 | C500-ID218 | INPUT UNIT | 2 |
148 | K3TR-NB11A | Meter Panel Mount | 1 |
149 | FND-X12H-SRT | Driver | 1 |
150 | FND-X50H | POSITION DRIVER 200-240VAC 50/60Hz 26W | 1 |
151 | NT20M-DT131 | 200-240VAC 50/60Hz 23W | 6 |
152 | NT20S-ST121-V2 | INTERACTIVE DISPLAY 24V DC 10W | 1 |
153 | NT20S-ST121-V3 | INTERACTIVE DISPLAY 24V DC 10W | 1 |
154 | NT20S-ST122-V1 | INTERACTIVE DISPLAY 24V DC 10W | 1 |
155 | NT31-ST122-V2 | INTERACTIVE DISPLAY 24V DC 15W | 1 |
156 | R88M-U10030HA-G11BJ | AC SERVO MOTOR 100W 200V 0.87A | 1 |
157 | R88M-U75030HA | SERVO MOTOR | 1 |
158 | FN515-II001 | IOC UNIT | 1 |
159 | FN515-NC022 | NC UNIT | 6 |
160 | FN515-CPU01APM | CPU UNIT | 3 |
161 | FN515-PS221 | PS UNIT | 3 |
162 | FN515-M1000H | PC UNIT | 3 |
163 | C200H-OD218 | Output Unit PLC 24VDC | 3 |
164 | C200H-ID216 | Input Unit PLC | 4 |
165 | C200HW-PA204S | Power Supply Unit AC100-120/200-240V 120VA 50/60Hz | 1 |
166 | CS1G-CPU42-EV1 | CPU UNIT | 1 |
167 | V530-C300 | V530-C300 | 1 |
168 | V700-CD1D-V3 | V700-CD1D-V3 | 1 |
169 | S8PS-10024CD | POWER SUPPLY 24VDC 4.5A | 1 |
170 | TL-N5ME1 | TLN5ME1 PROXIMITY SWITCH | 6 |
171 | E5CN-C2T | TEMPERATURE CONTROLLER | 2 |
172 | E5CN-C2TC | TEMPERATURE CONTROLLER | 21 |
173 | E5CZ-Q2 | TEMPERATURE CONTROLLER | 3 |
174 | E5CZ-Q2 | TEMPERATURE CONTROLLER | 1 |
175 | E5CN-C2MP-500 | TEMPERATURE CONTROLLER | 28 |
176 | E5CK-AA1 | DIGITAL CONTROLLER | 4 |
177 | E5ZE-8AQHD1TCB | E5ZE-8AQHD1TCB | 40 |
178 | E5ZE-8AQHD1TCB | E5ZE-8AQHD1TCB | 20 |
179 | E5ZE-8AQHD1TCB | E5ZE-8AQHD1TCB | 16 |
180 | F160-C10 | OMRON VISION SYSTEM | 3 |
181 | CS1W-BC103 | Base Unit PLC 10-Slot NIB | 1 |
182 | E6C2-CWZ6C | Rotary Encoder | 1 |
183 | RJLG-55 | SHUTTER VIDEO CAMERA(CCD) | 2 |
184 | S82H-3524 | Output Power Supply 24VDC | 1 |
185 | H3BA | 50/60Hz 100/110/120VAC 5A 250VAC | 2 |
186 | H3BA-8 | 24 VDC | 2 |
187 | H5CR-B | CORPORATION 240VAC~50/60Hz 5.5VA | 3 |
188 | H3CR-F | TIMER H3CRF8 100-240VAC | 1 |
189 | CVM1-PA208 | Power Supply Unit 100-120/200-240VAC 50/60Hz 150HA | 1 |
190 | C500-NC111-EV1 | NC UNIT (FOR PULSE OUTPUT) 3G2A5-NC111-EV1 | 6 |
191 | CVM1-BIO64 | OMRON | 1 |
192 | CVM1-PA208 | Power Supply Unit 100-120/200-240VAC 50/60Hz 150HA | 1 |
193 | CVM1-CPU01-V2 | CPU UNIT | 2 |
194 | CV500-SLK21 | SYSMAC LINK UNIT | 1 |
195 | C500-NC222 | NC UNIT | 1 |
196 | C500-NC112 | SYSMAC PROGRAMMABLE CONTROLLER-NC UNIT (FOR PULSE OUTPUT). | 2 |
197 | CVM1-BC103 | BASE UNIT 10 SLOT | 1 |
198 | C500-OD415CN | SYSMAC PROGRAMMABLE CONTROLLER-OUTPUT UNIT | 1 |
199 | C500-OD415CN | SYSMAC PROGRAMMABLE CONTROLLER-OUTPUT UNIT | 2 |
200 | C500-OD213 | Output Module | 1 |
201 | C500-ID219 | Input Unit SYSMAC PLC 24V 7mA | 1 |
202 | CVM1-PA208 | POWER SUPPLY UNIT XLNT CONDITION | 1 |
203 | CVM1-CPU21-V2 | Programmable Controller | 1 |
204 | C500-DRM21-V1 | CPU | 1 |
205 | C500-SLK21 | Omron Sysmac Link Unit | 1 |
206 | C500-NC222 | NC UNIT | 1 |
207 | C500-OD213 | PLC Output Unit | 3 |
208 | C500-ID219 | Input Unit SYSMAC PLC 24V 7mA | 3 |
209 | C500-ID218 | INPUT UNIT | 1 |
210 | CV500-BC101 | Base Unit | 1 |
211 | CVM1-BC103 | BASE UNIT 10 SLOT | 1 |
212 | C500-NC112 | SYSMAC PROGRAMMABLE CONTROLLER-NC UNIT (FOR PULSE OUTPUT) | 2 |
213 | C500-ID218 | INPUT UNIT | 2 |
214 | C500-OD219 | INPUT MODULE | 1 |
215 | C500-OD415CN | SYSMAC PROGRAMMABLE CONTROLLER-OUTPUT UNIT | 1 |
216 | C500-MD211CN | I/O MOD 16 24V | 1 |
217 | C500-OD213 | PLC Output Unit | 1 |
218 | FND-X06L | Servo Position Driver | 1 |
219 | R88D-UA10LA | AC Servo Driver 110V | 15 |
220 | R88D-UA12LA | AC Servo Drive | 14 |
221 | C500-MD211CN | I/O MOD 16 24V | 1 |
222 | GT1-BSC01 | Digital unit 24 VDC | 1 |
223 | GT1-DA04MX | Analog unit 24VDC 0.1A | 6 |
224 | S82K-24024 | POWER SUPPLY INPUT:AC100-120V 5.5A OUTPUT:200-230V 3.5A | 3 |
225 | R88D-HS22 | AC SERVO DRIVER 100-220VAC 50/60Hz 65va | 1 |
226 | R88D-H306G | AC SERVO DRIVER 200/220VAC 2700VA | 1 |
227 | R88D-HS04 | AC SERVO DRIVER 100-220VAC 65VA | 1 |
228 | R88D-HS10 | AC SERVO DRIVER 100-220VAC 65VA | 1 |
Sure-Flow pH 獨有的暢通的液接界設(shè)計使其不僅適用于一般pH測量,在測量粘稠樣品及膠體是也能展示及性能。測量間隔,只需按壓電極帽末端讓電極填充液流出即可方便清洗液接界。該電極適用于Star系列儀表,采用的BNWP接口具有良好的防水性和安全性。BNWP接口與標(biāo)準(zhǔn)BNC接口一樣適用于大部分pH計。BNC 接口, 電纜線長1 m. 電極帽直徑16 mm.推薦使用: 背景復(fù)雜的微量樣品(低至0.2mL),科學(xué)研究,質(zhì)量控制
技術(shù)參數(shù) pH 測量范圍 | 0-14 |
溫度測量范圍 | 0-100°C |
參比結(jié)構(gòu) | ROSS |
液接界 | Sure-Flow |
電極尺寸 | 165 mm x 6 mm (6mm 部分長95 mm) |
pH 精度 | 0.01 |
電極填充液 | 3 M KCI |
OPTEX FA光電傳感器,分為通用型光電傳感器和專用型光電傳感器兩大系列。專用型傳感器主要有:激光位移傳感器、圖像傳感器、透明體檢測型、背景抑制型、光纖放大器型、色標(biāo)檢測型等。 OPTEX FA光電傳感器在物流機械、包裝機械、陶瓷機械、食品機械、噴碼機械、貼標(biāo)機械、充填機械、造紙機械、車庫行業(yè)等得到廣泛應(yīng)用。 透明體檢測型: KR-Q50N/P KR-Q150N/P SR-Q50N/P KR-Q50CN/P KR-Q150CN/P SR-Q50CN/P 圖像傳感器: CVS1-N10 CVS1-N20 CVS1-N40 CVS1-P10 CVS1-P20 CVS1-P40 VRF放大器系列: VRF-N VRF-P VRF-HN VRF-A VRF-TN/Z1N VRF-TP/Z1P VRF-CN VRF-CP VRF-CA NF光纖系列: NF-DM01 NF-DM02 NF-DS06 NF-DK06 NF-DK04 NF-DB01 NF-DB03 NF-DK4Z NF-DK63Z NF-DR01 NF-DR02 NF-DR03 NF-DR04 NF-DR06 NF-KD43 NF-DV01 NF-DV02 NF-DV03 NF-DK33 NF-DF03 NF-DF02 NF-TB02 NF-TK05 NF-TK77 NF-TR01 NF-TR02 NF-TR03 NF-TB03 高精度位移傳感器: CD1-30N CD1-100N CD1-250N CD3-30N CD3-100N CD3-250N OPTEX紅外線測溫儀: 防水耐沖擊構(gòu)造PT-5LD PT-7LD (測溫范圍0~500度 -30~200度) BS-30TA BS-05TA BS-02TA BS-30TV BS-05TV BS-02TV(測溫范圍0~500度) 激光同軸型PT-303(測溫范圍-20~400度) 電子元件測溫利器PT-3S(測溫范圍0~200度) 穿透火焰專用型VF-3000(測溫范圍400~3000度) 鐳射照準(zhǔn)指引PT-305 (測溫范圍-40~510度) GT-950(測溫范圍-32~950度) 在線式非接觸溫度計BF-30GO-A BF-30G1-A (測溫范圍400~1200度) 固定式BA-30TA BA-30TV BA-30TC BA-06TA BA-06TV BA-06TC(測溫范圍0~500度) |
4WMM10M50,4WMM16M50,4WMM6P50,4WMM10P50,4WMM16P50,4WMM6Q50,4WMM10Q50,手動換向閥,手動換向閥生產(chǎn)廠家,手動換向閥價格,拓森手動換向閥
UG00C-T UG00C-T 富士POD下載電纜 |
UG221H-LE4 5.7寸 STN 單色 320*240 24VDC |
UG221H-LR4 5.7寸 STN 單色 320*240 SRAM/時鐘 |
UG221H-SR4 5.7寸 STN 16色 320*240 24VDC |
UG221H-TC4 5.7寸 TFT 16色 320*240 24VDC |
UG320HD-SC4 7.7寸 STN 128色 640*480 手持型 |
UG330H-VH4 8.4寸 TFT 800*600 32768色 以太網(wǎng) 高性能型 24VDC |
UG330H-VS4 8.4寸 TFT 800*600 32768色 標(biāo)準(zhǔn)型 24VDC |
UG330H-SS4 7.7寸 STN 640*480 128色 標(biāo)準(zhǔn)型 24VDC |
UG430H-SS1 10.4寸TFT 640*480 128 色 220VAC |
UG430H-TH1 10.4寸TFT 640*480 32768色 以太網(wǎng) 高性能型 220V |
UG430H-TH4 10.4寸TFT 640*480 32768色 以太網(wǎng) 高性能型 24VDC |
UG430H-TS1 10.4寸TFT 640*480 32768色 標(biāo)準(zhǔn)型 220VAC |
UG430H-TS4 10.4寸TFT 640*480 32768色 標(biāo)準(zhǔn)型 24VDC |
UG430H-VH1 10.4寸TFT 800*600 32768色 以太網(wǎng) 高性能型 220V |
UG430H-VH4 10.4寸TFT 800*600 32768色 以太網(wǎng) 高性能型 24VDC |
UG430H-VS1 10.4寸TFT 800*600 32768色 標(biāo)準(zhǔn)型 220V |
UG430H-VS4 10.4寸TFT 800*600 32768色 標(biāo)準(zhǔn)型24VDC |
UG530H-VH1 12.1寸TFT 800*600 32768色 以太網(wǎng) 高性能型 220V |
UG530H-VH4 12.1寸TFT 800*600 32768色 以太網(wǎng) 高性能型24VDC |
UG530H-VS1 12.1寸TFT 800*600 32768色 標(biāo)準(zhǔn)型 220V |
UG530H-VS4 12.1寸TFT 800*600 32768色 標(biāo)準(zhǔn)型 24VDC |
作用機制:
潮霉素B是由吸水鏈霉菌(Streptomyces hygroscopicus)代謝產(chǎn)生的一種氨基糖苷類抗生素,通過抑制蛋白質(zhì)合成而殺死細菌、真菌和高等真核細胞。潮霉素B通過干擾70S核糖體易位和誘導(dǎo)對mRNA模板的錯讀而抑制蛋白合成。目前常用于篩選和維持培養(yǎng)含潮霉素抗性基因的原核或真核細胞。
抗性基因:
對潮霉素B的抗性源自編碼潮霉素B磷酸轉(zhuǎn)移酶(Hph)的基因。至今發(fā)現(xiàn)兩種來源:
► Streptomyces hygroscopicus產(chǎn)生的Hph抗性基因;
► Escherichia coli,Klebsiella pneumoniae內(nèi)質(zhì)粒攜帶的Hph抗性基因(常用);
生物應(yīng)用:
1) 篩選和維持培養(yǎng)穩(wěn)定轉(zhuǎn)染Hph+載體的原核細胞或真核細胞(植物細胞和哺乳動物細胞);
2) 由于作用模式的差異,常與G418 (GeneticinTM),Zeocin™ 和blasticidin S聯(lián)合使用,篩選穩(wěn)定轉(zhuǎn)染兩個不同載體的細胞株;
| |||
抗生素 | 抗性機制 | 抗性基因 | 哺乳動物篩選濃度 |
潮霉素B | 干擾70S核糖體易位和誘導(dǎo)對mRNA模板的錯讀 | Hph | 200~500μg/mL |
G418 | 干擾80S核糖體功能和抑制蛋白合成 | Tn5/Tn601 | 100~1000μg/mL |
Zeocin | 摻入或者切割DNA引起細胞死亡 | Sh ble | 50~100μg/mL |
blasticidin S | 核糖體上抑制肽鍵形成 | Bsd/BSD | 3~50μg/mL |
3)抗病毒劑,由于潮霉素B選擇性滲透進入因病毒感染增強通透性的細胞,而且具有抑制翻譯的功效;
4)作為驅(qū)蟲藥加入動物飼料;
應(yīng)用濃度:
潮霉素B用來篩選穩(wěn)轉(zhuǎn)株的工作濃度需要根據(jù)細胞類型,培養(yǎng)基,生長條件和細胞代謝率而變化。推薦使用濃度為50-1000 μg/mL,最佳濃度需要殺滅曲線來確定。
哺乳動物細胞·200-500 μg/mL;細菌/植物細胞·100-300 μg/mL;真菌·300-1000 μg/mL;
產(chǎn)品使用:
使用一:殺滅曲線確定最佳殺死濃度(僅作參考,因個人檢測體系而異)
(1)往組織培養(yǎng)級的96孔板內(nèi)加入未轉(zhuǎn)染細胞,細胞密度約50-200細胞/孔;細胞貼壁之后,往每孔加入含不同濃度(如50-1000μg/mL,至少5個濃度)潮霉素B的200μL新鮮培養(yǎng)基;
(2)37℃,5% CO2培養(yǎng)箱孵育細胞10-14天;
(3)培養(yǎng)5-7天后更換新的培養(yǎng)液,培養(yǎng)液內(nèi)含有相應(yīng)濃度的潮霉素B;
(4)10-14天后使用細胞增殖方法如MTT,CCK-8等評估細胞活力;也可以通過檢測細胞克隆數(shù)或百分比匯合率來確定毒性效應(yīng)。一般選擇在10-14天能夠殺死所有細胞的最小濃度為最佳篩選濃度。
使用二:篩選穩(wěn)定轉(zhuǎn)染細胞
(1) 對于貼壁細胞,直接吸去60mm培養(yǎng)皿內(nèi)的轉(zhuǎn)染細胞培養(yǎng)基,然后加入含有潮霉素B的新鮮培養(yǎng)基5-6ml;對于懸浮細胞,無菌條件250x g,離心10min除去舊的轉(zhuǎn)染細胞培養(yǎng)基,然后懸浮在約5ml的含潮霉素B的新鮮培養(yǎng)基;
(2) 5-7天后,如上方法更換含有潮霉素B的新鮮培養(yǎng)基;
(3) 再孵育細胞5-7天;
(4) 10-14天孵育后,培養(yǎng)體系中只含有能夠表達潮霉素B抗性表型的活細胞。因此篩選之后如步驟一,更換不含潮霉素B的新鮮培養(yǎng)基。
PH是水質(zhì)監(jiān)測與控制的最基本的理化參數(shù)之一,對工廠生產(chǎn)、飲用水,廢水的監(jiān)測都有著重要的作用。
1。功能與參數(shù)測量范圍:0~14
2。PH 精確度 :土0.01
3。PH 分辨率:土0.01
4。PH 溫度補償: 自動或手動
5。輸出:4~20
6。MA通訊:RS232/485
7。顯示字符:LED顯示 2行16位
SPA4865LW進口三角帶一級代理商,空調(diào)機皮帶,耐高溫三角帶,工業(yè)皮帶(窄V帶),耐高溫皮帶,空調(diào)機皮帶,高速防油窄型帶,進口三星三角帶,高速傳動帶,進口三星風(fēng)機皮帶,防靜電三角帶,三星高速防油三角帶等世界名優(yōu)工業(yè)皮帶亞太地區(qū)總代理。
三角帶的每一個型號規(guī)定了三角帶的斷面尺寸,A型三角帶的斷面尺寸是:頂端寬度13mm、厚度為8mm;B型三角帶的斷面尺寸是:頂端寬度17MM,厚度為10.5MM;C型三角帶的斷面尺寸是:頂端寬度22MM,厚度為13.5MM;D型三角帶的斷面尺寸是:頂端寬度21.5MM,厚度為19MM;E型三角帶的斷面尺寸是:頂端寬度38MM,厚度為25.5MM。對應(yīng)尺寸(寬*高):O(10*6)、A(12.5*9)、B(16.5*11)、C(22*14)、D(21.5*19)、E(38*25.5)。
不同型號的皮帶輪的槽角在不同直徑范圍下的推薦皮帶輪槽角度數(shù)如下:O型皮帶輪在帶輪直徑范圍在50mm~71mm時為34度;在71mm~90mm時為36度, >90mm時為38度; A型皮帶輪在帶輪直徑范圍在71mm~100mm時為34度,100mm~125mm時為36度;>125mm時為38度; B型皮帶輪在帶輪直徑范圍在 125mm~160mm時為34度;160mm~200mm時為36度,>200mm時為38度; C型皮帶輪在帶輪直徑范圍在200mm~250mm時為34度,250mm~315mm時為36度,>315mm時為38度;D型皮帶輪在帶輪直徑范圍在 355mm~450mm時為36度,>450mm時為38度;E型 500mm~630mm時為36度,>630mm時為38度。
SPA4300LW代表什么?SPA代表三角帶型號,4300代表長度。三角帶的型號有:Y Z A B C D E這幾種,從Y到E,節(jié)寬依次變大。 國標(biāo)GB/T1154-1997中有詳細解釋。Lw見下:產(chǎn)品名;普通三角帶、窄V帶組成材質(zhì):橡膠、拉力骨架線作用:印刷機械、紡織機械、工廠設(shè)備、動力設(shè)備、汽車傳動、農(nóng)機傳動、等設(shè)備的機械傳動
Lw有效長度 Li 內(nèi)周長度通常型號有: 普通三角帶截型SIZE截面尺寸(Transect size) 可供規(guī)格內(nèi)周長長度(Providable inner-perimeter)
優(yōu)點1,結(jié)構(gòu)簡單,制造、安裝精度要求不高,使用維護方便, 三角帶適用于兩軸中心距較大的場合2,傳動平穩(wěn),噪音低,有緩沖吸振作用3,過載時,傳動帶會在帶輪上打滑,可以防止薄弱零件的損壞,起安全保護作用
pcb定位燈