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 應(yīng)用領(lǐng)域：

◆監(jiān)測個(gè)人輻射暴露

◆地區(qū)或周界監(jiān)測

◆監(jiān)測輻射泄漏和污染

◆確保符合管理部門規(guī)定

◆監(jiān)測本底輻射的變化

◆核物理原理演示

◆檢查輻射性礦物

儀器的工作原理：

　　每次射線穿過蓋革管并引起電離時(shí)，蓋革計(jì)數(shù)管會產(chǎn)生一脈沖電流。每個(gè)脈沖都是電子探測并進(jìn)行運(yùn)算。探測器以你選擇的模式顯示計(jì)算：CPM，mR/hr，或者總計(jì)。在s1單位中，使用CPS和μsv/hr。檢測器探測出來的計(jì)數(shù)數(shù)字由于放射能的任意狀態(tài)而每分鐘都在變化。以過去一段時(shí)間內(nèi)的平均值表示更加準(zhǔn)確，而且這段時(shí)間越長數(shù)據(jù)越準(zhǔn)確。

技術(shù)指標(biāo)：

◆劑量率：0.001-110.0mR/hr或0.01-1100μSV/ hr

◆計(jì)數(shù)：CPM ：0-350,000或CPS：0-3,500總數(shù)：1-9,999,000

◆精度：±10% 典型值 ±15% 最大(mR/Hr和μSV/ hr模式下)

◆計(jì)時(shí)器：長達(dá)40小時(shí)

◆靈敏度：1000cpm/mR/Hr，參比Cs137

◆顯示：四位數(shù)值液晶顯示，含模式指示

◆報(bào)警：內(nèi)置，當(dāng)輻射水平達(dá)到用戶設(shè)定值時(shí)發(fā)出聲音報(bào)警；

　　　　用戶可調(diào)報(bào)警水平達(dá)50mR/Hr和160,000CPM

◆計(jì)數(shù)燈：每次計(jì)數(shù)時(shí)LED燈閃爍

◆聲音：每次計(jì)數(shù)時(shí)發(fā)出BP機(jī)似的聲音，可靜音

◆電源：9V 堿性電池，一般背景水平下可用2160小時(shí)；1mR/Hr靜音時(shí)可用625小時(shí)

◆選項(xiàng)：電纜和軟件

◆尺寸：150×80×30mm

◆重量(克)：225g

◆認(rèn)證：CE

武漢壽力壓縮機(jī)配件服務(wù)中心

手機(jī)：18971404606周先生

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001168溫控閥芯02250092-081溫控閥芯250028-762溫控閥芯88290002-832溫控閥芯013697溫控閥芯88290022-023溫控閥芯02250096-738溫控閥芯88290001-006溫控閥總成250025-621溫控閥維修包02250105-553溫控閥維修包001084溫控閥維修包001076溫控閥維修包826502-144溫控閥O型圈02250101-372溫控閥V密封圈250017-280背壓調(diào)節(jié)開關(guān)02250127-402減壓閥048354減壓閥406929壓力調(diào)節(jié)器408275壓力調(diào)節(jié)器02250084-027壓力調(diào)節(jié)器02250046-568壓力調(diào)節(jié)器048059壓力調(diào)節(jié)器045099壓力調(diào)節(jié)器02250052-358壓力調(diào)節(jié)器88291002-741壓力調(diào)節(jié)器250023-600真空調(diào)節(jié)器02250190-454溢流閥048409壓力調(diào)節(jié)器維修包250019-453壓力調(diào)節(jié)器維修包041742壓力調(diào)節(jié)器維修包02250049-634放空閥02250100-042放空閥250028-693放氣閥250025-655放氣閥250030-276放氣閥041111放氣閥409783放空閥250031-771放空閥維修包250031-772放空閥維修包02250045-132放空閥維修包001667放空閥維修包250020-353壽力控維修包608311-001螺旋閥維修包250006-526進(jìn)氣閥彈簧02250112-032控制管路過濾器408389控制管路過濾器02250112-031控制管路過濾器維修包001692控制管路過濾器維修包250031-245控制管路過濾器維修包02250115-960控制管路過濾器維修包88290018-880控制管路過濾器維修包02250117-782回油管過濾器維修包02250125-684電磁閥250038-666電磁閥250038-755電磁閥88290015-219電磁閥250038-672電磁閥02250125-657電磁閥250038-676電磁閥02250125-665電磁閥250038-673電磁閥維修包250038-730電磁閥線圈250031-738電磁閥線圈02250055-940電磁閥維修包250033-821最小壓力閥02250097-598最小壓力閥02250097-609最小壓力閥242405最小壓力閥88290016-467最小壓力閥88290017-107最小壓力閥88290017-287最小壓力閥88290017-432最小壓力閥02250046-338最小壓力閥組件88290017-286止回閥88290018-146最小壓力閥維修包250026-758最小壓力閥維修包02250177-150最小壓力閥維修包88290009-185進(jìn)氣閥02250110-998最小壓力閥維修包250018-262最小壓力閥維修包250019-444最小壓力閥維修包001176最小壓力閥維修包001177最小壓力閥維修包250018-456最小壓力閥維修包250018-459最小壓力閥維修包88290018-146最小壓力閥維修包88290019-707最小壓力閥維修包88290012-468進(jìn)氣閥維修包250042-656彈簧02250056-278進(jìn)氣閥維修包02250046-396壓力閥蓋維修包02250048-363壓力閥O型圈包02250051-337壓力閥活塞維修包250014-656真空開關(guān)250017-991水壓開關(guān)250017-992水壓開關(guān)02250084-534水溫開關(guān)02250050-154壓差開關(guān)02250100-095高溫保護(hù)開關(guān)250039-909溫度傳感器88290001-535急停按扭88290003-806壓力傳感器88290014-514壓力傳感器241771V型過濾器241772V型過濾器維修包040694壓力調(diào)節(jié)開關(guān)407778壓力調(diào)節(jié)開關(guān)250029-249進(jìn)氣閥維修包250017-451進(jìn)氣閥維修包250019-451進(jìn)氣閥維修包02250053-273進(jìn)氣閥維修包250031-438進(jìn)氣閥維修包02250073-277進(jìn)氣閥維修包02250155-971進(jìn)氣閥維修包88290003-548進(jìn)氣閥02250130-050進(jìn)氣閥02250088-348進(jìn)氣閥02250060-988進(jìn)氣閥88291006-018進(jìn)氣閥02250083-783進(jìn)氣閥02250045-626進(jìn)氣閥02250141-182進(jìn)氣閥02250155-970進(jìn)氣閥88290009-185進(jìn)氣閥041110進(jìn)氣閥88291004-492進(jìn)氣閥049905單向閥02250115-272單向閥02250110-557單向閥408893往復(fù)閥02250051-538電腦板88290008-999電腦板88290007-999電腦板02250106-360電腦板88290007-789電路板88320001-052控制器041006消聲器040758消聲器043386燈泡88290006-089紅色燈泡88290006-090綠色燈泡02250049-258軸封600893-001軸封02250057-037軸封02250050-363軸封02250050-364軸封02250123-809軸封868430-030三通826502-210O型圈826502-214O型圈826502-240O型圈826502-426O型圈88842026-051O型圈88842035-047O型圈88842035-023O型圈88842035-061O型圈88842035-065O型圈88842035-075O型圈88842030-044O型圈02250099-630尼龍管02250054-861尼龍管02250052-841管芯02250099-052管芯88290001-129汽缸044912循環(huán)卸荷閥046782循環(huán)卸荷閥維修包02250046-568減壓閥040691壓力表02250117-009壓力表250005-185壓力表02250100-095溫度開關(guān)02250136-694溫度表02250050-154壓差開關(guān)02250117-009壓力表88290001-219視油鏡88290004-380排氣壓力表250042-010計(jì)時(shí)器88290006-159溫度表88290005-932阻塞指示器88290005-481安全閥88290005-480安全閥88290001-476安全閥88290005-469安全閥88290005-468安全閥88290005-471安全閥88290005-472安全閥88290005-473安全閥88290001-950安全閥88290005-479安全閥88290011-135移動(dòng)機(jī)安全閥88290010-713移動(dòng)機(jī)安全閥250024-654安全閥250029-330安全閥02250097-349安全閥88290001-466空濾88290001-467空濾88290002-337空濾88290002-338空濾88290003-111空濾88290001-469空濾88290004-372空濾88290007-018空濾406631聯(lián)軸器彈性元件250004-641聯(lián)軸器彈性元件250018-551聯(lián)軸器彈性元件867308-250聯(lián)軸器螺栓88290003-312聯(lián)軸器彈性元件88290003-404聯(lián)軸器彈性元件02250152-670聯(lián)軸器彈性元件88290019-861聯(lián)軸器彈性元件88290009-996聯(lián)軸器彈性元件  88290019-862聯(lián)軸器彈性元件250004-638聯(lián)軸器02250111-222聯(lián)軸器88290009-988聯(lián)軸器88290004-374聯(lián)軸器88290009-989聯(lián)軸器88290013-848聯(lián)軸器88290009-988聯(lián)軸器88290009-992聯(lián)軸器88290009-989聯(lián)軸器88290001-904移動(dòng)機(jī)聯(lián)軸器88290006-808移動(dòng)機(jī)聯(lián)軸器橡膠88290003-397聯(lián)軸器橡膠88290003-322聯(lián)軸器橡膠88290003-326聯(lián)軸器橡膠88290010-075聯(lián)軸器橡膠88290010-079聯(lián)軸器橡膠02250115-957最小壓力閥蝶閥02250127-402減壓閥88290017-745氣水分離器88290003-393氣水分離器88290001-403氣水分離器88290003-109氣水分離器02250111-238氣水分離器88290003-209氣水分離器88290004-746氣水分離器88290013-186氣水分離器88290020-910氣水分離器88290023-106氣水分離器88290017-431氣水分離器88290001-040氣水分離器02250127-121氣水分離器 02250081-108維修包02250078-841氣水分離器88290010-023電子排水閥88290010-699電子排水閥88290012-751電子排水閥88290010-561電子排水閥88290012-684電子排水閥88290012-779電子排水閥配件88290012-780電子排水閥定時(shí)器02250081-108水分離器維修包02250087-422水分離器維修包044374水分離器維修包250004-642壓縮機(jī)端連接轂250018-005電機(jī)端連接轂02250125-775節(jié)流孔02250098-303球閥250039-909溫度傳感器02250058-087溫度傳感器02250048-457溫度傳感器001104移動(dòng)機(jī)溫度開關(guān)250039-910傳感器88290003-806傳感器88290004-947風(fēng)冷組合冷卻器88290005-380風(fēng)冷冷卻器88290005-200風(fēng)冷冷卻器88291001-504風(fēng)冷冷卻器88290017-243風(fēng)冷冷卻器88290020-670冷卻器88290004-083水冷油冷卻器88290003-414冷卻器88290003-413冷卻器88290001-549冷卻器88290002-198冷卻器88290006-633冷卻器88290003-019冷卻器88290002-536冷卻器88290001-549冷卻器88290002-817水冷油冷卻器88290002-818冷卻器02250096-706風(fēng)冷冷卻器88290002-457冷卻器88290002-458冷卻器88290003-120風(fēng)冷冷卻器88290017-242風(fēng)冷冷卻器88290006-640風(fēng)冷冷卻器88290012-760風(fēng)冷油氣冷卻器總成88290012-803風(fēng)冷油冷卻器88290007-938風(fēng)冷后冷卻器88290006-982風(fēng)冷空油冷卻器88290001-357水冷油冷卻器88290001-358水冷后冷卻器88290009-786水冷冷卻器88290006-636冷卻器88290006-635冷卻器88290003-025水冷油冷卻器88290003-026水冷后冷卻器88290004-345水冷后冷卻器02250111-201水冷后冷卻器02250111-202水冷油冷卻器88290014-166油冷卻器88290013-028后冷卻器88290004-346水冷油卻器88290006-645后冷卻器88290006-644油冷卻器88290004-532冷卻器88290004-533油冷卻器88290004-534空氣冷卻器88290006-382不銹鋼水冷油冷88290003-114油冷卻器88290006-643風(fēng)冷空油冷卻器88290009-734風(fēng)冷冷卻器88290006-634風(fēng)冷空油冷卻器02250094-744水冷油冷卻器250017-527水冷后冷卻器040680水冷后冷卻器042950水冷后冷卻器88290005-930水冷油冷卻器88290020-662水冷后冷卻器88290020-661水冷油冷卻器88290004-347水冷后冷卻器88290020-663水冷油冷卻器88290014-478風(fēng)冷冷卻器88290015-765風(fēng)冷冷卻器88290014-225風(fēng)冷冷卻器88291002-894-00風(fēng)冷冷卻器88291007-378風(fēng)冷冷卻器88290012-331風(fēng)冷冷卻器88290013-517風(fēng)冷冷卻器88290013-267風(fēng)冷冷卻器88290013-518風(fēng)冷冷卻器88290013-269風(fēng)冷冷卻器88290013-519風(fēng)冷冷卻器88291006-08888291006-99588290007-939冷卻器02250150-059移動(dòng)機(jī)冷卻器02250124-822移動(dòng)機(jī)冷卻器02250166-257移動(dòng)機(jī)冷卻器88290002-664移動(dòng)機(jī)油冷88290001-901移動(dòng)機(jī)發(fā)動(dòng)機(jī)冷卻器88290019-198水冷后冷卻器88290019-197水冷油冷卻器040550橡膠彎頭88290004-441金屬軟管88290002-396金屬軟管88290002-392金屬軟管88290001-396金屬軟管02250097-827金屬軟管88290006-985金屬軟管88290006-983金屬軟管88290013-020金屬軟管02250110-538金屬軟管88290001-579金屬軟管88290001-398金屬軟管88290019-517金屬軟管88290006-685金屬軟管88290001-895金屬軟管88290001-893金屬軟管88290003-567金屬軟管88290005-550金屬軟管88290004-444軟管88290004-445軟管88290004-446軟管88290004-646軟管02250127-905軟管88290017-244軟管02250098-622軟管02250098-624軟管02250143-476軟管02250141-104軟管88290009-266軟管88290009-268軟管88290009-267軟管88290001-425軟管88290001-426軟管88290005-227軟管88290005-228軟管88290002-328軟管88290002-330軟管88290002-331放油軟管88290002-332軟管88290003-395軟管88290005-540軟管88290005-541軟管88290005-542軟管88290005-545軟管040413回油軟管88290015-902皮帶88290001-042橡膠軟管249608-021移動(dòng)機(jī)軟管02250054-260移動(dòng)機(jī)軟管88290006-685移動(dòng)機(jī)軟管88290012-445進(jìn)氣軟管049625進(jìn)氣軟管020169接頭02250101-490接頭883727-012接頭883527-012接頭250016-351啟動(dòng)按鈕250003-798油分離器壓差表250003-799油過濾器壓差表040029加油蓋88290006-366視油鏡046559視油鏡88290002-666風(fēng)扇電機(jī)88290007-878風(fēng)扇電機(jī)88290001-755風(fēng)扇電機(jī)88290009-879風(fēng)扇88290004-940電動(dòng)機(jī)88290002-576電動(dòng)機(jī)250003-797空濾器真空表250022-632高壓停機(jī)開關(guān)250034-304傳動(dòng)軸250034-305傳動(dòng)軸02250077-596傳動(dòng)軸02250094-411齒輪02250094-735齒輪499097-188齒輪499096-188齒輪490054-206齒輪490055-206齒輪145-25800齒輪145-25700齒輪250039-253排水器88290001-732時(shí)間繼電器88320001-035壓力開關(guān)041076止逆閥88290002-649綠色按鈕88290002-651紅色按鈕88290015-538急停按扭02250049-934油濾底座040136球絞接頭02250084-094溫度開關(guān)250041-638機(jī)油壓力開關(guān)88290012-827機(jī)油壓力傳感器88290012-829機(jī)油溫度傳感器88290010-831張緊輪軸88290010-830張緊輪042148壓差指示器88290003-022減震墊88290007-005油氣分離器桶88290014-226油氣分離器桶88290006-100壓力開關(guān)88290001-302風(fēng)扇熱繼電器88290015-740單向交流電壓數(shù)顯表 

衛(wèi)星天線 慣性導(dǎo)航系統(tǒng) 天線 微波射頻儀器 通訊儀器儀表 光通訊儀器儀表 光學(xué)儀器儀表 光通訊儀器儀表

德國沃申道夫WACHENDORFF編碼器     德國Wachendorff（沃申道夫）品牌由Rolf Wachendorff（沃申道夫） 于1978年11月17日在德國的Geisenheim創(chuàng)建，至今已有20多年的發(fā)展進(jìn)程。德國Wachendorff（沃申道夫）自動(dòng)化公司為客戶提供堅(jiān)固的工業(yè)元件及智能化系統(tǒng)，專用在高精度的測量位移、角度及方向的工業(yè)場合中。堅(jiān)固的工業(yè)級Wachendorff（沃申道夫）編碼器的正常質(zhì)保期為五年，遠(yuǎn)遠(yuǎn)高于歐洲大部分品牌的一年質(zhì)保期的期限。     其他工業(yè)電子產(chǎn)品。     具體產(chǎn)品有：     Opus 系列　 OPUS A1 , OPUS A2 , OPUS Thor ,OPUS 21     WDG　 系列　 WDG 58B , WDG SSI , WDG 40A , WDG 80H , WDG 58V , WDG 58K, WDG 115T , WDG 115M 等     WDG 58B-1500-ABN-G24-K3　　　　 WDG 40A-500-ABN-I24-K2     WDG 53S-5000-ABN-I24-K2　　　　 WDG 90B-1024-AB-H30-S2     WDG 115T-2048-AB-R30-S3　　　　 WDG 115M-1800-ABN-G24-S3     SZG 65-1250-N-UP-G24-05　　　　 WDG-DPB1B-0013-C100-0CC     WDG-SL00G-1213-C100-CRW　　　　 WDG-S100G-0013-B150-PRL     WDG-C8B1B-0013-C100-CRW　　　　 WDGHA1005　　　　　 WDGHA1210

Wachendorff（沃申道夫）公司的核心價(jià)值觀一直是為控制、檢測、優(yōu)化工控系統(tǒng)設(shè)計(jì)生產(chǎn)銷售設(shè)備。Wachendorff（沃申道夫）以產(chǎn)品廣泛應(yīng)用于惡劣的環(huán)境而贏得美譽(yù)，同時(shí)公司產(chǎn)品也大量應(yīng)用于便攜式或可移動(dòng)的操作平臺。 

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 Rat Interleukin 10（IL-10）  

ELISA Kit

Catalog No. CSB-E04595r

（96T）

l          This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma ａnd Tissue Homogenates.

l          Expiration date   six months from the date of manufacture

l          FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well ａnd incubated. Then a TMB （3,3＇,5,5＇ tetramethyl-benzidine） substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody ａnd enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.

SPECIFICITY

This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.

SENSITIVITY

The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.

The sensitivity of this assay, or Lower Limit of Detection （LLD） was defined as the lowest protein concentration that could be differentiated from zero.

MATERIALS PROVIDED

STORAGE

1.      Unopened test kits should be stored at 2-8°C upon receipt ａnd the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.

2.      Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above. 

3.      A microtiter plate reader with a bandwidth of 10 nm or less ａnd an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.

REAGENT PREPARATION

Bring all reagents to room temperature before use.

1.        Wash Buffer  If crystals have formed in the concentrate, warm up to room temperature ａnd mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.

2.        Standard  Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard （200 pg/ml）. The Sample Diluent serves as the zero standard （0 pg/ml）. Prepare fresh for each assay. Use within 4 hours ａnd discard after use.

3.        Biotin-antibody  Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent（1:100）, respectively.

4.        HRP-avidin  Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent（1:100）, respectively.

Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, ａnd clothing protection when using this material.

OTHER SUPPLIES REQUIRED

—          Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

—          Pipettes ａnd pipette tips.

—          Deionized or distilled water.

—          Squirt bottle, manifold dispenser, or automated microplate washer.

—          An incubator which can provide stable incubation conditions up to 37°C±0.5°C.

SAMPLE COLLECTION AND STORAGE

l          Serum  Use a serum separator tube （SST） ａnd allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum ａnd assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Plasma  Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot ａnd store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l          Tissue Homogenates   100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS ａnd stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed ａnd removed immediately. Alternatively, aliquot ａnd store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

ASSAY PROCEDURE

Bring all reagents ａnd samples to room temperature before use. It is recommended that all samples, standards, ａnd controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.

1.        Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.

2.        Remove the liquid of each well, don’t wash.

3.        Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature ａnd mix gently until solution appears uniform.

4.        Aspirate each well ａnd wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer （200μl） ａnd let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.

5.        Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.

6.        Repeat the aspiration ａnd wash five times as step 4.

7.        Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts ａnd other temperature fluctuations in the dark.

8.        Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.

9.        Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.

CALCULATION OF RESULTS

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.

Average the duplicate readings for each standard, control, ａnd sample ａnd subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ａnd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. ａnd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

LIMITATIONS OF THE PROCEDURE

—          The kit should not be used beyond the expiration date on the kit label.

—          Do not mix or substitute reagents with those from other lots or sources.

—          It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.

—          If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent ａnd repeat the assay.

—          Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, ａnd kit age can cause variation in binding.

—          This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

TECHNICAL HINTS

—          Centrifuge vials before opening to collect contents.

—          When mixing or reconstituting protein solutions, always avoid foaming.

—          To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, ａnd between reagent additions. Also, use separate reservoirs for each reagent.

—          When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.

—          To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

—          Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.

—          Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

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