IW5221 便充式長壽工作燈 LED
適用場所: IW5221 便充式長壽工作燈 廣泛適用于鐵路、電力、消防、船舶等以及各種室內(nèi)外移動照明。
結(jié)構(gòu)特性: ●IW5221 便充式長壽工作燈 采用大功率超高亮度的固態(tài)免維護光源,光效高、壽命長,使用壽命長達10萬小時,永不 需要更換光源?!馡W5221 便充式長壽工作燈 高能無記憶電池,容量大,自放電率低,經(jīng)濟環(huán)保,一次充滿電后可連續(xù)工作10小時以上,既可滿足長時間當班的需要,也可作為停電應(yīng)急照明?!馡W5221 便充式長壽工作燈 內(nèi)置交、直流兩種充電方式,即可接車載點煙器充電,也可接市電進行充電;減少了攜帶充電器的煩惱,極大地方便了客戶的使用?!馡W5221 便充式長壽工作燈 外殼采用進口防彈膠材料,強度高,抗沖擊性能好。●IW5221 便充式長壽工作燈 防水防腐,外殼防護等級達到IP65,確保燈具可在各種惡劣條件下長期可靠工作?!馡W5221 便充式長壽工作燈 特殊的保護電路設(shè)計,防止電池過充、過放;極大的提高了電池的使用壽命。 ●IW5221 便充式長壽工作燈 外形美觀,結(jié)構(gòu)緊湊、攜帶方便,可手提、腰胯,也可放置臺面進行照明,燈頭可在180°范圍內(nèi)上下調(diào)整照射角度。
西門子伺服模塊6FC5203/6FC5210/6FC5247/6FC5410
歡迎來電 15818404692 肖思敏
主營產(chǎn)品: 西門子數(shù)控、伺服系統(tǒng):6SN 、6FX、6FC等;西門子伺服電機:1FT、1FK、1PH等;西門子直流調(diào)速器及備件(晶閘管、可控硅、EUPEC、IGBT、電容、電阻、風機等):6SE70、6RY、6RY、6SY70、6SY80 等西門子變頻器備件(西門子晶閘管、西門子可控硅、西門子EUPEC、西門子IGBT、西門子電容、西門子電阻、西門子風機,變頻器風機等):6SE70、6RY28等;西門子低壓:3RT、3RV、3RH、3RP、3RA、3RB、3RN、3RS、3RX、3TC、3TK、3TD、3TG、3TH43、3LD、3TF68-69、3NE、3NC、3UG、3TX、7PU 3KA、3SB、3VL、3VF、3RG、4AV、3WN6、3KE、3LF、5SX、5SG等等.
奧地利Kraus&Naimer(K&N)中字藍系列開關(guān):CA10-A007 、A058、A200、A201、A211、CA20、KG、KH、C43-316、L350-L2000等
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歡迎來電 15818404692 肖思敏
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10.16/04/180 AG BK BX1813910000 BUZ 10.16/04/180F AG BK BX1964320000 BUZ 10.16/04/180F AG BK BX PRT1850650000 BUZ 10.16/04/180SF AG BK BX1813840000 BUZ 10.16/05/180 AG BK BX1813920000 BUZ 10.16/05/180F AG BK BX1849260000 BUZ 10.16/05/180F AG BK BX TB1849280000 BUZ 10.16/05/180F AG BK BX TB1850660000 BUZ 10.16/05/180SF AG BK BX1813850000 BUZ 10.16/06/180 AG BK BX1813930000 BUZ 10.16/06/180F AG BK BX1850670000 BUZ 10.16/06/180SF AG BK BX1813860000 BUZ 10.16/07/180 AG BK BX1813940000 BUZ 10.16/07/180F AG BK BX1849300000 BUZ 10.16/07/180F AG BK BX TB1850680000 BUZ 10.16/07/180SF AG BK BX1813870000 BUZ 10.16/08/180 AG BK BX1813950000 BUZ 10.16/08/180F AG BK BX1850690000 BUZ 10.16/08/180SF AG BK BX1813880000 BUZ 10.16/09/180 AG BK BX1813960000 BUZ 10.16/09/180F AG BK BX1850700000 BUZ 10.16/09/180SF AG BK BX1848220000 BUZ 10.16/3F AG SW SO1924540000 BUZ 10.16HP/02/180 AG BK BX1924620000 BUZ 10.16HP/02/180F AG BK BX1924700000 BUZ 10.16HP/02/180SF AG BK BX1924550000 BUZ 10.16HP/03/180 AG BK BX1924630000 BUZ 10.16HP/03/180F AG BK BX1924710000 BUZ 10.16HP/03/180SF AG BK BX1924560000 BUZ 10.16HP/04/180 AG BK BX1924640000 BUZ 10.16HP/04/180F AG BK BX1924720000 BUZ 10.16HP/04/180SF AG BK BX1924570000 BUZ 10.16HP/05/180 AG BK BX1924650000 BUZ 10.16HP/05/180F AG BK BX1924740000 BUZ 10.16HP/05/180SF AG BK BX1924580000 BUZ 10.16HP/06/180 AG BK BX1924660000 BUZ 10.16HP/06/180F AG BK BX1924750000 BUZ 10.16HP/06/180SF AG BK BX1924590000 BUZ 10.16HP/07/180 AG BK BX1924670000 BUZ 10.16HP/07/180F AG BK BX1924760000 BUZ 10.16HP/07/180SF AG BK BX1924600000 BUZ 10.16HP/08/180 AG BK BX1924680000 BUZ 10.16HP/08/180F AG BK BX1924770000 BUZ 10.16HP/08/180SF AG BK BX1924610000 BUZ 10.16HP/09/180 AG BK BX1924690000 BUZ 10.16HP/09/180F AG BK BX1924780000 BUZ 10.16HP/09/180SF AG BK BX1937590000 BV/SV 7.62HP KO1937570000 BV/SV 7.62HP/02 ARPL GR1937550000 BV/SV 7.62HP/02 ZE GR1937580000 BV/SV 7.62HP/04 ARPL GR1937560000 BV/SV 7.62HP/04 ZE GR1937600000 BV/SV7.62HP MOFU GR1928610000 BVL 7.62HP/02/180 3.5SN BK BX1941200000 BVL 7.62HP/02/180 3.5SN BK BX1928730000 BVL 7.62HP/02/180FI 3.5SN BK BX1928840000 BVL 7.62HP/02/180SFI 3.5SN BK BX1929300000 BVL 7.62HP/02/270 3.5SN BK BX1929410000 BVL 7.62HP/02/270FI 3.5SN BK BX1929520000 BVL 7.62HP/02/270SFI 3.5SN BK BX1006600000 BVL 7.62HP/02/90 3.5SN BK1928280000 BVL 7.62HP/02/90 3.5SN BK BX1928390000 BVL 7.62HP/02/90FI 3.5SN BK BX1928500000 BVL 7.62HP/02/90SFI 3.5SN BK BX1928620000 BVL 7.62HP/03/180 3.5SN BK BX1941210000 BVL 7.62HP/03/180 3.5SN BK BX1928740000 BVL 7.62HP/03/180FI 3.5SN BK BX1928850000 BVL 7.62HP/03/180SFI 3.5SN BK BX1929310000 BVL 7.62HP/03/270 3.5SN BK BX1929420000 BVL 7.62HP/03/270FI 3.5SN BK BX1929530000 BVL 7.62HP/03/270SFI 3.5SN BK BX1928290000 BVL 7.62HP/03/90 3.5SN BK BX1928400000 BVL 7.62HP/03/90FI 3.5SN BK BX1928510000 BVL 7.62HP/03/90SFI 3.5SN BK BX1928630000 BVL 7.62HP/04/180 3.5SN BK BX1941220000 BVL 7.62HP/04/180 3.5SN BK BX1928750000 BVL 7.62HP/04/180FI 3.5SN BK BX1928860000 BVL 7.62HP/04/180SFI 3.5SN BK BX1929320000 BVL 7.62HP/04/270 3.5SN BK BX1929430000 BVL 7.62HP/04/270FI 3.5SN BK BX1929540000 BVL 7.62HP/04/270SFI 3.5SN BK BX1928300000 BVL 7.62HP/04/90 3.5SN BK BX1006620000 BVL 7.62HP/04/90FI 3.5SN BK1928410000 BVL 7.62HP/04/90FI 3.5SN BK BX1928520000 BVL 7.62HP/04/90SFI 3.5SN BK BX1928650000 BVL 7.62HP/05/180 3.5SN BK BX1928760000 BVL 7.62HP/05/180FI 3.5SN BK BX1928870000 BVL 7.62HP/05/180SFI 3.5SN BK BX1929330000 BVL 7.62HP/05/270 3.5SN BK BX1929440000 BVL 7.62HP/05/270FI 3.5SN BK BX1929550000 BVL 7.62HP/05/270SFI 3.5SN BK BX1928310000 BVL 7.62HP/05/90 3.5SN BK BX1928420000 BVL 7.62HP/05/90FI 3.5SN BK BX1928530000 BVL 7.62HP/05/90SFI 3.5SN BK BX1928660000 BVL 7.62HP/06/180 3.5SN BK BX1928770000 BVL 7.62HP/06/180FI 3.5SN BK BX1928900000 BVL 7.62HP/06/180SFI 3.5SN BK BX1929340000 BVL 7.62HP/06/270 3.5SN BK BX1929450000 BVL 7.62HP/06/270FI 3.5SN BK BX1929560000 BVL 7.62HP/06/270SFI 3.5SN BK BX1928320000 BVL 7.62HP/06/90 3.5SN BK BX1928430000 BVL 7.62HP/06/90FI 3.5SN BK BX1928540000 BVL 7.62HP/06/90SFI 3.5SN BK BX1928670000 BVL 7.62HP/07/180 3.5SN BK BX1928780000 BVL 7.62HP/07/180FI 3.5SN BK BX1928910000 BVL 7.62HP/07/180SFI 3.5SN BK BX1929350000 BVL 7.62HP/07/270 3.5SN BK BX1929460000 BVL 7.62HP/07/270FI 3.5SN BK BX1929570000 BVL 7.62HP/07/270SFI 3.5SN BK BX1928330000 BVL 7.62HP/07/90 3.5SN BK BX1928440000 BVL 7.62HP/07/90FI 3.5SN BK BX1928550000 BVL 7.62HP/07/90SFI 3.5SN BK BX1928680000 BVL 7.62HP/08/180 3.5SN BK BX1928790000 BVL 7.62HP/08/180FI 3.5SN BK BX1928920000 BVL 7.62HP/08/180SFI 3.5SN BK BX1929360000 BVL 7.62HP/08/270 3.5SN BK BX1929470000 BVL 7.62HP/08/270FI 3.5SN BK BX1929580000 BVL 7.62HP/08/270SFI 3.5SN BK BX1928340000 BVL 7.62HP/08/90 3.5SN BK BX1928450000 BVL 7.62HP/08/90FI 3.5SN BK BX1928560000 BVL 7.62HP/08/90SFI 3.5SN BK BX1928690000 BVL 7.62HP/09/180 3.5SN BK BX1928800000 BVL 7.62HP/09/180FI 3.5SN BK BX1928930000 BVL 7.62HP/09/180SFI 3.5SN BK BX1929370000 BVL 7.62HP/09/270 3.5SN BK BX1929480000 BVL 7.62HP/09/270FI 3.5SN BK BX1929590000 BVL 7.62HP/09/270SFI 3.5SN BK BX1928350000 BVL 7.62HP/09/90 3.5SN BK BX1928460000 BVL 7.62HP/09/90FI 3.5SN BK BX1928570000 BVL 7.62HP/09/90SFI 3.5SN BK BX1928700000 BVL 7.62HP/10/180 3.5SN BK BX1928810000 BVL 7.62HP/10/180FI 3.5SN BK BX1928940000 BVL 7.62HP/10/180SFI 3.5SN BK BX1929380000 BVL 7.62HP/10/270 3.5SN BK BX1929490000 BVL 7.62HP/10/270FI 3.5SN BK BX1929600000 BVL 7.62HP/10/270SFI 3.5SN BK BX1928360000 BVL 7.62HP/10/90 3.5SN BK BX1928470000 BVL 7.62HP/10/90FI 3.5SN BK BX1928580000 BVL 7.62HP/10/90SFI 3.5SN BK BX1928710000 BVL 7.62HP/11/180 3.5SN BK BX1928820000 BVL 7.62HP/11/180FI 3.5SN BK BX1928950000 BVL 7.62HP/11/180SFI 3.5SN BK BX1929390000 BVL 7.62HP/11/270 3.5SN BK BX1929500000 BVL 7.62HP/11/270FI 3.5SN BK BX1929610000 BVL 7.62HP/11/270SFI 3.5SN BK BX1928370000 BVL 7.62HP/11/90 3.5SN BK BX1928480000 BVL 7.62HP/11/90FI 3.5SN BK BX1928590000 BVL 7.62HP/11/90SFI 3.5SN BK BX1928720000 BVL 7.62HP/12/180 3.5SN BK BX1928830000 BVL 7.62HP/12/180FI 3.5SN BK BX1928960000 BVL 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CD0252111748 CLI C 02-3 GE/SW . MP1568241742 CLI C 02-3 GE/SW / CD1568241750 CLI C 02-3 GE/SW : CD0252111750 CLI C 02-3 GE/SW : MP1568241738 CLI C 02-3 GE/SW + CD0252111738 CLI C 02-3 GE/SW + MP1568241751 CLI C 02-3 GE/SW = CD0252111751 CLI C 02-3 GE/SW = MP1568241502 CLI C 02-3 GE/SW 0 CD0252111502 CLI C 02-3 GE/SW 0 MP0252101502 CLI C 02-3 GE/SW 0 RL1568241505 CLI C 02-3 GE/SW 1 CD0252111505 CLI C 02-3 GE/SW 1 MP0252101505 CLI C 02-3 GE/SW 1 RL1568241508 CLI C 02-3 GE/SW 2 CD0252111508 CLI C 02-3 GE/SW 2 MP1568241511 CLI C 02-3 GE/SW 3 CD0252111511 CLI C 02-3 GE/SW 3 MP1568241514 CLI C 02-3 GE/SW 4 CD0252111514 CLI C 02-3 GE/SW 4 MP1568241517 CLI C 02-3 GE/SW 5 CD0252111517 CLI C 02-3 GE/SW 5 MP0252101517 CLI C 02-3 GE/SW 5 RL1568241520 CLI C 02-3 GE/SW 6 CD0252111520 CLI C 02-3 GE/SW 6 MP1568241523 CLI C 02-3 GE/SW 7 CD0252111523 CLI C 02-3 GE/SW 7 MP1568241526 CLI C 02-3 GE/SW 8 CD0252111526 CLI C 02-3 GE/SW 8 MP1568241529 CLI C 02-3 GE/SW 9 CD0252111529 CLI C 02-3 GE/SW 9 MP1568241637 CLI C 02-3 GE/SW A CD1568241056 CLI C 02-3 GE/SW ? CD1568241055 CLI C 02-3 GE/SW ? CD0252111637 CLI C 02-3 GE/SW A MP0252111700 CLI C 02-3 GE/SW a MP0252111697 CLI C 02-3 GE/SW ? MP0252111699 CLI C 02-3 GE/SW ? MP1568241752 CLI C 02-3 GE/SW AC CD0252111752 CLI C 02-3 GE/SW AC MP1568241735 CLI C 02-3 GE/SW ? CD1568241639 CLI C 02-3 GE/SW B CD0252111639 CLI C 02-3 GE/SW B MP0252111701 CLI C 02-3 GE/SW b MP1568241641 CLI C 02-3 GE/SW C CD0252111641 CLI C 02-3 GE/SW C MP0252111702 CLI C 02-3 GE/SW c MP1568241643 CLI C 02-3 GE/SW D CD0252111643 CLI C 02-3 GE/SW D MP0252111703 CLI C 02-3 GE/SW d MP1568241645 CLI C 02-3 GE/SW E CD0252111645 CLI C 02-3 GE/SW E MP0252111704 CLI C 02-3 GE/SW e MP1568241746 CLI C 02-3 GE/SW ET CD0252111746 CLI C 02-3 GE/SW ET MP1568241647 CLI C 02-3 GE/SW F CD0252111647 CLI C 02-3 GE/SW F MP0252111705 CLI C 02-3 GE/SW f MP1568241649 CLI C 02-3 GE/SW G CD0252111649 CLI C 02-3 GE/SW G MP0252111706 CLI C 02-3 GE/SW g MP1568241651 CLI C 02-3 GE/SW H CD0252111651 CLI C 02-3 GE/SW H MP0252111707 CLI C 02-3 GE/SW h MP1568241653 CLI C 02-3 GE/SW I CD0252111653 CLI C 02-3 GE/SW I MP0252111708 CLI C 02-3 GE/SW i MP1568241655 CLI C 02-3 GE/SW J CD0252111655 CLI C 02-3 GE/SW J MP0252111709 CLI C 02-3 GE/SW j MP1568241657 CLI C 02-3 GE/SW K CD0252111657 CLI C 02-3 GE/SW K MP0252111710 CLI C 02-3 GE/SW k MP1568241659 CLI C 02-3 GE/SW L CD0252111659 CLI C 02-3 GE/SW L MP0252111711 CLI C 02-3 GE/SW l MP1568241661 CLI C 02-3 GE/SW M CD0252111661 CLI C 02-3 GE/SW M MP0252111712 CLI C 02-3 GE/SW m MP1568241663 CLI C 02-3 GE/SW N CD0252111663 CLI C 02-3 GE/SW N MP0252111713 CLI C 02-3 GE/SW n MP1568241665 CLI C 02-3 GE/SW O CD1568241057 CLI C 02-3 GE/SW ? CD1568241736 CLI C 02-3 GE/SW ? CD0252111665 CLI C 02-3 GE/SW O MP0252111714 CLI C 02-3 GE/SW o MP0252111695 CLI C 02-3 GE/SW ? MP1568241667 CLI C 02-3 GE/SW P CD0252111667 CLI C 02-3 GE/SW P MP0252111715 CLI C 02-3 GE/SW p MP1568241747 CLI C 02-3 GE/SW PET. CD0252111747 CLI C 02-3 GE/SW PET. MP1568241669 CLI C 02-3 GE/SW Q CD0252111669 CLI C 02-3 GE/SW Q MP0252111716 CLI C 02-3 GE/SW q MP1568241671 CLI C 02-3 GE/SW R CD0252111671 CLI C 02-3 GE/SW R MP0252111717 CLI C 02-3 GE/SW r MP1568241673 CLI C 02-3 GE/SW S CD0252111673 CLI C 02-3 GE/SW S MP0252111718 CLI C 02-3 GE/SW s MP1568241676 CLI C 02-3 GE/SW T CD0252111676 CLI C 02-3 GE/SW T MP0252111719 CLI C 02-3 GE/SW t MP1568241679 CLI C 02-3 GE/SW U CD0252111679 CLI C 02-3 GE/SW U MP0252111720 CLI C 02-3 GE/SW u MP1568241681 CLI C 02-3 GE/SW V CD0252111681 CLI C 02-3 GE/SW V MP0252111721 CLI C 02-3 GE/SW v MP1568241683 CLI C 02-3 GE/SW W CD0252111683 CLI C 02-3 GE/SW W MP0252111722 CLI C 02-3 GE/SW w MP1568241687 CLI C 02-3 GE/SW X CD0252111687 CLI C 02-3 GE/SW X MP0252111723 CLI C 02-3 GE/SW x MP1568241696 CLI C 02-3 GE/SW Y CD0252111696 CLI C 02-3 GE/SW Y MP
紅外測溫控徑儀是一種基于紅外輻射原理的非接觸式測量儀表,它能將進入測量視場內(nèi)的特殊波段紅外輻射能量量化,并相應(yīng)地轉(zhuǎn)化為可以識別和利用的電信號。由于晶體的生長直徑和生長長度可通過調(diào)整旋轉(zhuǎn)速度和提拉速度來控制,穩(wěn)定的溫度是硅晶棒均勻生長的重要因素,KJY型紅外測溫控徑儀解決了硅晶體生長過程中的品質(zhì)控制和直徑控制問題。
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人細胞毒性T淋巴細胞相關(guān)抗原4(CTLA-4/CD152)ELISA分析試劑盒說明書用于測定血清、血漿及相關(guān)液體樣本中的含量。試劑盒嚴格按照說明書的操作進行,試劑不同批號組分不得混用。
人細胞毒性T淋巴細胞相關(guān)抗原4(CTLA-4/CD152)ELISA分析試劑盒說明書說明書價格,ELISA Kit,人細胞毒性T淋巴細胞相關(guān)抗原4(CTLA-4/CD152)ELISA分析試劑盒說明書,品牌elisa試劑盒哪家好代測服務(wù)。我司憑借國內(nèi)外先進的科技水平以及新老客戶長期以來對我們的支持,在生物科研領(lǐng)域取得了長足的發(fā)展。歡迎廣大客戶前來選購!
elisa試劑盒廠家參數(shù)規(guī)格:
中文名稱:人細胞毒性T淋巴細胞相關(guān)抗原4(CTLA-4/CD152)ELISA分析試劑盒說明書,品牌elisa試劑盒哪家好
英文名稱:CagAAb ELISA kit
規(guī)格:96T/48T
性狀:液體
存儲:4℃保存6個月
運輸方式:快遞發(fā)貨
檢測標本:血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等
檢測方法:雙抗夾心法、間接法、競爭法等。
歡迎訪問我們的網(wǎng)站,請給我們電話或留言,告訴我您的需要,并留下您寶貴的建議和意見。如需更多ELISA法測定試劑盒產(chǎn)品請點擊這里。
上海嵐派生物ELISA試劑盒廠家主營如下:
試劑盒:大鼠Elisa試劑盒,小鼠Elisa試劑盒,人Elisa試劑盒,犬Elisa試劑盒,魚Elisa試劑盒,雞Elisa試劑盒,牛Elisa試劑盒、其他動物類Elisa試劑盒、植物Elisa試劑盒、分子生物學試劑盒、 免疫組化試劑盒、金標檢測試劑盒、生化試劑盒、細胞凋亡試劑盒。
抗體:一抗、二抗、進口原裝抗體、進口分裝抗體、單克隆抗體、多克隆抗體、單標抗體、雙標抗體、多標抗體;免疫組化抗體、免疫細胞化學抗體、免疫熒光抗體、流式細胞抗體??贵w:一抗、二抗、進口原裝抗體、進口分裝抗體、單克隆抗體、多克隆抗體、單標抗體、雙標抗體、多標抗體;免疫組化抗體、免疫細胞化學抗體、免疫熒光抗體、流式細胞抗體。
動物血清:內(nèi)皮細胞專用血清、GIBCO胎牛清、HyClone。動物血清:內(nèi)皮細胞專用血清、GIBCO胎牛清、HyClone。
細胞:原裝進口細胞、正常細胞、腫瘤細胞、腫瘤耐藥細胞。細胞:原裝進口細胞、正常細胞、腫瘤細胞、腫瘤耐藥細胞。
酶聯(lián)免疫吸附測定enzyme linked immunosorbent assay(簡寫ELISA)指將可溶性的抗原或抗體結(jié)合到聚苯乙烯等固相載體上,利用抗原抗體結(jié)合專一性進行免疫反應(yīng)的定性和定量檢測方法。酶聯(lián)免疫吸附測定(ELISA)為免疫學中的經(jīng)典實驗。
elisa試劑盒廠家,酶聯(lián)免疫吸附測定試劑盒工作原理:
1971年Engvall和Perlmann發(fā)表了酶聯(lián)免疫吸附劑測定(enzyme linked immunosorbent assay,ELISA)用于IgG定量測定的文章,使得1966年開始用于抗原定位的酶標抗體技術(shù)發(fā)展成液體標本中微量物質(zhì)的測定方法。這一方法的基本原理是:①使抗原或抗體結(jié)合到某種固相載體表面,并保持其免疫活性。②使抗原或抗體與某種酶連接成酶標抗原或抗體,這種酶標抗原或抗體既保留其免疫活性,又保留酶的活性。在測定時,把受檢標本(測定其中的抗體或抗原)和酶標抗原或抗體按不同的步驟與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復合物與其他物質(zhì)分開,最后結(jié)合在固相載體上的酶量與標本中受檢物質(zhì)的量成一定的比例。加入酶反應(yīng)的底物后,底物被酶催化變?yōu)橛猩a(chǎn)物,產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)顏色反應(yīng)的深淺刊物定性或定量分析。由于酶的催化效率很高,故可極大地放大反應(yīng)效果,從而使測定方法達到很高的敏感度。
elisa試劑盒廠家,酶聯(lián)免疫吸附測定試劑盒使用方法:
夾心法
夾心法常用于檢測大分子抗原,一般之操作步驟為:
1、將具有專一性之抗體固著(coating)于塑膠孔盤上,完成后洗去多余抗體;
2、加入待測檢體,檢體中若含有待測之抗原,則其會與塑膠孔盤上的抗體進行專一性鍵結(jié);
3、洗去多余待測檢體,加入另一種對抗原專一之一次抗體,與待測抗原進行鍵結(jié);
4、洗去多余未鍵結(jié)一次抗體,加入帶有酵素之二次抗體,與一次抗體鍵結(jié);
5、洗去多余未鍵結(jié)二次抗體,加入酵素受質(zhì)使酵素呈色,以肉眼或儀器讀取呈色結(jié)果。
間接法
間接法常用于檢測抗體,一般之操作步驟為:
1、將已知之抗原固著于塑膠孔盤上,完成后洗去多余之抗原;
2、加入待測檢體,檢體中若含有待測之一次抗體,則其會與塑膠孔盤上的抗原進行專一性鍵結(jié);
3、洗去多余待測檢體,加入帶有酵素之二次抗體,與待測之一次抗體鍵結(jié);
4、洗去多余未鍵結(jié)二次抗體,加入酵素受質(zhì)使酵素呈色,藉儀器(ELISA reader)測定塑膠盤中的吸光值(OD值),以評估有色終產(chǎn)物的含量即可測量待測抗體的含量。
競爭法
競爭法是一種較少用到的ELISA檢測機制,一般用于檢測小分子抗原,其操作步驟為:
1、將具有專一性之抗體固著于塑膠孔盤上,完成后洗去多余抗體;
2、加入待測檢體,使檢體中的待測抗原與塑膠孔盤上的抗體進行專一性鍵結(jié);
3、加入帶有酵素之抗原,此抗原也可與塑膠孔盤上的抗體進行專一性鍵結(jié),由于塑膠孔盤上固著的抗體數(shù)量有限,因此當檢體中抗原的量越多,則帶有酵素之抗原可鍵結(jié)的固著抗體就越少,亦即,兩種抗原皆競相與塑膠孔盤上抗體鍵結(jié),即所謂競爭法之由來;
4、洗去檢體與帶有酵素之抗原,加入酵素受質(zhì)使酵素呈色,當檢體中抗原量越多,代表塑膠孔盤內(nèi)留下之帶有酵素的抗原越少,顯色也就越淺。
當需要偵測無法獲得兩種以上單一性抗體的抗原,或是不易得到足夠的純化抗體以固著于孔盤上時,一般會考慮使用競爭法ELISA。
elisa試劑盒廠家,酶聯(lián)免疫吸附測定試劑盒結(jié)果判斷:
定性測定
定性測定的結(jié)果判斷是對受檢標本中是否含有待測抗原或抗體作出"有"或"無"的簡單回答,分別用"陽性"、"陰性"表示。"陽性"表示該標本在該測定系統(tǒng)中有反應(yīng)。"陰性"則為無反應(yīng)。用定性判斷法也可得到半定量結(jié)果,即用滴度來表示反應(yīng)的強度,其實質(zhì)仍是一個定性試驗。在這種半定量測定中,將標本作一系列稀釋后進行試驗,呈陽性反應(yīng)的最高稀釋度即為滴度。根據(jù)滴度的高低,可以判斷標本反應(yīng)性的強弱,這比觀察不稀釋標本呈色的深淺判斷為強陽性、弱陽性更具定量意義。
在間接法和夾心法ELISA中,陽性孔呈色深于陰性孔。在競爭法ELISA中則相反,陰性孔呈色深于陽性孔。
定量測定
ELISA操作步驟復雜,影響反應(yīng)因素較多,特別是固相載體的包被難達到各個體之間的一致,因此在定量測定中,每批測試均須用一系列不同濃度的參考標準品在相同的條件下制作標準曲線。測定大分子量物質(zhì)的夾心法ELISA,標準曲線的范圍一般較寬,曲線最高點的吸光度可接近2.0,繪制時常用半對數(shù)紙,以檢測物的濃度為橫坐標,以吸光度為縱坐標,將各濃度的值逐點連接,所得曲線一般呈S形,其頭、尾部曲線趨于平坦,中央較呈直線的部分是最理想的檢測區(qū)域。
測定小分子量物質(zhì)常用競爭法,其標準曲線中吸光度與受檢物質(zhì)的濃度呈負相關(guān)。標準曲線的形狀因試劑盒所用模式的差別而略有不同。ELISA測定的標準曲線注意圖中橫坐標為對數(shù)關(guān)系,這更有利于測定系統(tǒng)的表達。
現(xiàn)在訂購試劑盒享有更多優(yōu)惠活動,提供免費待測服務(wù),全程提供技術(shù)支持,產(chǎn)品質(zhì)量保證,性價比高,售后有保障,歡迎新老客戶咨詢訂購:人細胞毒性T淋巴細胞相關(guān)抗原4(CTLA-4/CD152)ELISA分析試劑盒說明書,品牌elisa試劑盒哪家好。
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