序號 | 公稱壓力 | 進口 | 出口 | 適用溫度 | 閥體材料 |
1 | 40MPa | 腰型法蘭 | 板式安裝 | -20~450℃ | 20# |
2 | -70~240℃ | 1Cr18Ni9Ti |
SPME操作平臺(含15ml瓶架)/15ml固相微萃取操作平臺簡介:美國Supelco公司專利產(chǎn)品-固相微萃取(Solid Phase Micro Extraction),1994年獲美國匹茲堡分析儀器會議R&D100項革新大獎,是一種應現(xiàn)代儀器的要求而產(chǎn)生的樣品前處理新技術,幾乎克服了以往一些傳統(tǒng)樣品處理技術的所有缺點,集采樣、萃取、濃縮、進樣于一體,便于攜帶,真正實現(xiàn)樣品的現(xiàn)場采集和富集,能夠與氣相、氣相-質(zhì)譜、液相、液相-質(zhì)譜儀聯(lián)用,有手動或自動兩種操作方式,讓更多的分析工作者從重復、煩瑣的操作中解脫出來。廣泛應用于環(huán)保及水質(zhì)處理、臨床藥理、公安案件分析、制藥、化工、國防等領域。固相微萃取(SPME)非常小巧,狀似一只色譜注射器,由手柄(Holder)和萃取頭或纖維頭(Fiber)兩部分構成。萃取頭是一根外套不銹鋼細管的1cm長、涂有不同色譜固定相或吸附劑的熔融石英纖維頭,纖維頭在不銹鋼管內(nèi)可自由伸縮,用于萃取、吸附樣品;手柄用于安裝或固定萃取頭,可永久使用。SPME操作平臺(含15ml瓶架)/15ml固相微萃取操作平臺?典型應用:* 表面活性劑* 環(huán)境水樣* 食品、香精、香料* 法庭樣品* 血、尿和體液中藥物* 聚合物和固樣中痕量雜質(zhì)(頂空方式)* 藥物中殘留溶劑* 氣體硫化物及揮發(fā)物(VOC)
銷售/租賃通訊測試儀8960|E5515C
名稱:無線通訊測試儀 8960|E5515C 廠家:Agilent 型號:8960|E515B|E5515C
描述
用于8960系列10無線通訊測試儀的Agilent E5515C硬件平臺為手機制造商提供直接的競爭優(yōu)勢。業(yè)經(jīng)驗證的8960無線通訊測試儀專為大規(guī)模、自動化手機生產(chǎn)測試而設計,具有快速、精確、可重復、多格式、易于編程和格式靈活的架構等特點。對于手機制造商來說,這有助于降低測試成本,提高產(chǎn)量,幫助他們滿足客戶當前和未來對手機的需求。
備注:
本公司進口電子儀器儀表的代理銷售、租賃和維修,成色新,價格低,以質(zhì)量為本,服務至誠!力求更,服務力求更! 歡迎來電 垂詢或親臨選購,并歡迎預定。
周經(jīng)理 135 6403 5149 021-6172 8576 / 021-61124 992
高價各類高頻儀器 儀表示波器、信號發(fā)生器、頻譜分析儀、網(wǎng)絡分析儀、直流穩(wěn)壓電源、手持示波表、邏輯分析儀、耐壓測試儀等各種測試儀器。
2015藥典要求用的封閉式薄膜過濾器一次購買可長期使用,每試驗一個濾頭損耗一張濾膜.其它部件均為耐用,多次使 用,無廢物處理,符合環(huán)保(GLP)要求.貴重藥液可以回收.在抗生素藥品檢查,所需培養(yǎng)基僅為封閉式濾器的一半量,大地節(jié)約試驗費用; 2聯(lián)、3聯(lián)、6聯(lián)封閉式薄膜過濾器配套,一次完成一批供試品試驗。
2015藥典要求用的封閉式薄膜過濾器規(guī)格:
1. 底座三聯(lián)式、六聯(lián)式均適合凈化臺內(nèi)操作.
2. 濾杯容積100ml,有50ml及100ml刻度.
2015藥典要求用的封閉式薄膜過濾器使用方法:
1.擰松底部圓螺母,抬起金屬固定架,取出玻璃桶,在多孔墊板下先放置一橡膠墊圈,在墊板上放置一聚四氟乙烯(PTFE)濾膜墊圈,然后放上微孔濾膜 (φ50mm,0.45μ),再放置一四氟乙烯(PTFE)濾膜墊圈,安裝玻璃桶,在玻璃桶頂部再安裝一片密封橡膠片,套上金屬固定架,擰緊底部螺母,蓋上頂部可啟開的蓋即成密閉式結構,如不需要全密閉結構,可不安裝頂部橡膠片;
2.底部排液管口接一橡膠管,并用紗布或別的包扎好管口;
3.將整個濾器連底座包扎好,進行121℃蒸汽消毒滅菌后即可使用;
4.加液體試樣,可用滅菌器注射,刺入橡膠片,注入濾器內(nèi),加固體、半固體試樣,可用開發(fā)式直接加入濾器內(nèi);
5.采用密閉培養(yǎng)時,請參考儀器操作說明;
Rat 15-LO/LOX ELISA Kit測定,大鼠血清15脂加氧酶 elisa試劑盒價格,15-LO/LOX實驗代檢測
ELISA種屬:人Human、大鼠Rat、小鼠Mouse、兔子Rabbit、雞Chicken、猴Monkey、牛Bovine、魚Fish、馬Equine、犬Canine、駱駝Camel、鹿Deer、植物Plant、山羊Goat、綿羊Sheep、豚鼠Guinea、鴨Duck、豬Porcine、驢Donkey等。
大鼠15脂加氧酶(15-LO/LOX)ELISA試劑盒 北京ELISA實驗代做 Rat 15-lipoxygenase,15-LO/LOX ELISA Kit 加拿大HCB公司ELISA試劑盒價格 北京代理 加拿大HCB進口原裝試劑盒,折扣低,貨期2-3周,原裝進口,質(zhì)量保證,國產(chǎn)ELISA試劑盒,外地用戶3天發(fā)貨,北京用戶可以送貨上門,詳詢: 15311986590 在線QQ:2694024752
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Wachendorff(沃申道夫)產(chǎn)品簡介及常用型號
Wachendorff(沃申道夫)主要的產(chǎn)品包括以下三個生產(chǎn)線:Opus 系列移動操作面板WDG 系列增量型編碼器 其他工業(yè)電子產(chǎn)品。具體產(chǎn)品有:Opus 系列 OPUS A1 , OPUS A2 , OPUS Thor ,OPUS 21WDG 系列 WDG 58B , WDG SSI , WDG 40A , WDG 80H , WDG 58V , WDG 58K, WDG 115T , WDG 115M 等 WDG 58B-1500-ABN-G24-K3 WDG 40A-500-ABN-I24-K2 WDG 53S-5000-ABN-I24-K2 WDG 90B-1024-AB-H30-S2 WDG 115T-2048-AB-R30-S3 WDG 115M-1800-ABN-G24-S3 SZG 65-1250-N-UP-G24-05 WDG-DPB1B-0013-C100-0CC WDG-SL00G-1213-C100-CRW WDG-S100G-0013-B150-PRL WDG-C8B1B-0013-C100-CRW WDGHA1005 WDGHA1210
詳細介紹:
WDG58B-1024-ABN-G24-L3德國沃申道夫WACHENDORFF
德國WACHENDORFF編碼器
Wachendorff 成立于1978年,是歐洲最大的編碼器生產(chǎn)廠家之一。幾十年來,Wachendorff公司的核心價值觀一直是為控制、檢測、優(yōu)化工控系統(tǒng)設計生產(chǎn)銷售設備。Wachendorff以產(chǎn)品廣泛應用于惡劣的環(huán)境而贏得美譽,同時公司產(chǎn)品也大量應用于便攜式或可移動的操作平臺。
德國WACHENDORFF編碼器
YM1151LT法蘭式液位變送器 | ||||||||
|
微處理器控制,頻率及其沖程可調(diào),工作時間可根據(jù)需要設定的,電磁驅動的隔膜式計量泵。可用于多種液體的計量輸送。
性能參數(shù)表
HT/HT-Z系列電磁隔膜計量泵性能參數(shù)表 | |||||||
產(chǎn)品名稱 | 流量(L/h) | 壓力(Bar) | 口徑(mm) | 沖程頻率(次/分鐘) | 功率(W) | 電壓(V) | 重量(kg) |
SDP-E0.5/12 | 0.5 | 12 | 6 | 120 | 35 | 220 | 2.8 |
SDP-E01/12 | 1 | 12 | 6 | 120 | 35 | 220 | 2.8 |
SDP-E03/8 | 3 | 8 | 6 | 120 | 46 | 220 | 2.8 |
SDP-E05/4 | 5 | 4 | 6 | 120 | 46 | 220 | 2.8 |
SDP-E07/4 | 7 | 4 | 6 | 120 | 46 | 220 | 2.8 |
SDP-E10/3 | 10 | 3 | 6 | 120 | 46 | 220 | 2.8 |
SDP-E12/2 | 12 | 2 | 6 | 120 | 50 | 220 | 2.8 |
SDP-E14/2 | 14 | 2 | 6 | 120 | 50 | 220 | 2.8 |
SDP-E18/2 | 18 | 2 | 6 | 120 | 50 | 220 | 2.8 |
SDP-E20/1.2 | 20 | 1.2 | 6 | 120 | 50 | 220 | 2.8 |
SDP-E25/1.2 | 25 | 1.2 | 6 | 120 | 50 | 220 | 2.8 |
SDP-E手動型:可調(diào)節(jié)沖程長度0-100%,可調(diào)節(jié)沖程頻率0-100%,可選液位開關功能。 | |||||||
SDP-EZ自動型:擁有手動功能外,可接受外部模擬量信號、脈沖信號進行自動控制 |
計量泵材質(zhì)表
SDP-E系列電磁隔膜計量泵材質(zhì)表 | |||||
配件名稱 Part name | 過流部分材質(zhì) Over-current part | ||||
泵頭材質(zhì) Pump head | 聚丙烯(RPP) | 聚氯乙烯(PVC) | 聚偏氟乙烯(PVDF) | SS304 | SS316 |
隔膜材質(zhì) Diaphragm | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE |
閥座材質(zhì) Valve seat | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE | 聚四氟乙烯PTFE | 316不銹鋼 | 316不銹鋼 |
閥球材質(zhì) Valve ball | 陶瓷ceramic | 陶瓷ceramic | 陶瓷ceramic | 陶瓷ceramic | 陶瓷ceramic |
吸入管 Inlet pipe | PVC/PE | PVC/PE | PE/PVDF | PE/PVDF | PE/PVDF |
吐出管 Outlet pipe | PE | PE | PE/PVDF | PE/PVDF | PE/PVDF |
斯得浦HL系列機械隔膜計量泵
產(chǎn)品特點Product features
.先進的結構緊湊牢固、使用壽命更長。.泵體選用優(yōu)質(zhì)的工程塑料,耐腐蝕性能更優(yōu)秀.泵頭可選擇PVDF/RPP/PVC/SUS304/SUS316等材質(zhì)應用范圍更廣。.加強型PTFE-橡膠多層復合隔膜、完全無泄漏、使用更放心。.運行或停泵時可進行0-100%的流量精確調(diào)節(jié)。.模塊化設計、保養(yǎng)維護更容易。產(chǎn)品應用Product application
.污水處理工程、電廠循環(huán)水處理、電鍍、線路板、造紙、游池等行業(yè)的聚合氯化鋁(PAC)、聚丙烯酰胺(PAM)、漂白劑等化學藥劑/助劑的精確投加。.石油、化工、實驗室等行業(yè)的化學藥劑的精確配比及投加.助磨劑及各類助燃劑的添加性能參數(shù)表
HL系列機械隔膜計量泵性能參數(shù)表 | ||||||
產(chǎn)品名稱 | 流量(L/h) | 壓力(Mpa) | 口徑(mm) | 電機功率(W) | 電壓(V) | 重量(kg) |
HL13/1.0 | 13 | 1 | 9X15軟管含注射閥及過濾器 | 40 | 380/220 | 5.8 |
HL26/1.0 | 26 | 1 | 9X15軟管含注射閥及過濾器 | 40 | 380/220 | 5.8 |
HL40/1.0 | 40 | 1 | 9X15軟管含注射閥及過濾器 | 40 | 380/220 | 5.8 |
HL55/1.0 | 55 | 1 | 9X15軟管含注射閥及過濾器 | 40 | 380/220 | 5.8 |
HL70/0.5 | 70 | 0.5 | 9X15軟管含注射閥及過濾器 | 60 | 380/220 | 5.8 |
HL100/0.3 | 100 | 0.3 | 9X15軟管含注射閥及過濾器 | 60 | 380/220 | 5.8 |
HL128/0.3 | 128 | 0.3 | 9X15軟管含注射閥及過濾器 | 60 | 380/220 | 5.8 |
HL-A150/0.3 | 150 | 0.3 | 9X15軟管含注射閥及過濾器 | 60 | 380 | 6 |
HL-A170/0.3 | 170 | 0.3 | 9X15軟管含注射閥及過濾器 | 60 | 380 | 6 |
HL-A200/0.3 | 200 | 0.3 | 9X15軟管含注射閥及過濾器 | 60 | 380 | 6 |
HL-A220/0.3 | 220 | 0.3 | 9X15軟管含注射閥及過濾器 | 90 | 380 | 6 |
注:本系列計量泵基本配置是9X15軟管連接,接口可另選擇:DN15PVC承插接口方式,和DN15法蘭盤連接方式。 |
計量泵過流部分材質(zhì)表
HL系列機械隔膜計量泵過流部分材質(zhì)表、 | ||||||
零配件名稱 | 過流部分材質(zhì) | |||||
泵 頭 Pump | RPP | PVC | PVDF | SUS304 | SUS316L | |
隔 膜 Diaphragm | PTFE+橡膠PTFE+Rubber | PTFE+橡膠PTFE+Rubber | PTFE+橡膠PTFE+Rubber | PTFE+橡膠PTFE+Rubber | PTFE+橡膠PTFE+Rubber | |
單 向 閥 Check Valves | RPP | PVC | PVDF | SUS304 | SUS316L | |
閥 球 座 Ball seat | PTFE | PTFE | PTFE | SUS304 | SUS316L | |
閥 球 Ball | 陶瓷Ceramics | 陶瓷Ceramics | 陶瓷Ceramics | 陶瓷Ceramics | 陶瓷Ceramics | |
密封墊片 Gasket | PTFE | PTFE | PTFE | PTFE | PTFE |
斯得浦HK系列機械隔膜計量泵
HK系列機械隔膜計量泵是可按各種工藝流程的需要,輸送水及類似水的化學液體以及腐蝕性和非腐蝕性液體的一種往復式容積泵,流量可在0-100%范圍內(nèi)調(diào)節(jié),產(chǎn)品執(zhí)行中國國家標準GB/T7782-2008及參照國際先進設計理念設計并生產(chǎn)。產(chǎn)品廣泛應用于石油、化工、水處理、環(huán)保、食品、輕工、造紙、制藥、印染、冶金、礦山、電鍍等行業(yè)。產(chǎn)品特點Product characteristics:
◆ 結構簡單緊湊,維護更加方便。◆ 雙偏心凸輪結構驅動,結構緊湊,運行平穩(wěn)可靠。◆ 油浸潤滑,只需定期更換潤滑油,潤滑系統(tǒng)無需專門維護。◆ 可靠的流量調(diào)節(jié)機構,產(chǎn)品運行或停止狀態(tài)時均可輕松調(diào)節(jié)流量。◆ 采用高強度的聚四氟乙烯與橡膠的復合膜片,耐腐蝕、壽命長、適合輸送各種腐蝕性、危險性液體。◆ 多種可選用的過流材質(zhì),還可按使用要求定制其它特殊材料,以適用輸送各種腐蝕性和非腐蝕性液體。◆ 完全不泄露,安全性能高,可輸送各種易燃、易爆、劇毒、放射性、強刺激性、強腐蝕性液體。◆ 高精度單向止回閥結構,具有計量精確、結構緊湊、密封性好、壽命長、互換性強、成本低、安裝方便等諸多優(yōu)點。◆ 可配套變頻電機接收4-20mA信號來調(diào)節(jié)流量,進行外部自動控制。◆ 可配套沖程調(diào)節(jié)器來自動調(diào)節(jié)流量。主要參數(shù)
◆ 額定流量24-2000L/h,最高額定壓力1.0Mpa。◆ 輸送介質(zhì)溫度-30-50℃(不銹鋼為100℃)。◆ 介質(zhì)粘度:0.3-800 mm2/s.◆ 計量復位精度+-1%。
性能參數(shù)表
HK系列機械隔膜計量泵性能參數(shù)表 | ||||||||
產(chǎn)品型號 Model | 最大流量Flow(L/h) | 最大出口壓力Pressure(Mpa) | 功率Power(W) | 電壓Voltage(V) | 泵頭材質(zhì)Pump head material | 口徑Diameter(mm) | 連接方式Connection | 重量Weight(kgs) |
HK24/1.0 |
24 |
1.0 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 16 |
HK54/1.0 | 54 |
1.0 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 16 |
HK85/0.8 | 85 |
0.8 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 16 |
HK100/0.8 | 100 |
0.8 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 16 |
HK120/0.8 | 120 |
0.8 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 17 |
HK175/0.7 | 175 |
0.7 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 17 |
HK210/0.6 |
210 |
0.6 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 17 |
HK280/0.6 | 280 |
0.6 |
250 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
承插socket | 17 |
HK320/0.6 | 320 |
0.6 | 370 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 17 |
HK380/0.5 | 380 |
0.5 | 370 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange |
16 |
HK410/0.5 |
410 | 0.5 |
550 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 18 |
HK500/0.4 |
500 |
0.4 |
550 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 18 |
HK550/0.4 | 550 | 0.4 |
550 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 18 |
HK600/0.3 | 600 | 0.3 |
550 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 18 |
HK630/0.3 | 630 | 0.3 |
550 |
380 |
RPP/PVDF、SS304(可選) |
DN15 |
法蘭flange | 18 |
HK-A800/0.4 |
800 |
0.4 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
50 |
HK-B1000/0.4 |
1000 |
0.4 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
50 |
HK-B1200/0.4 |
1200 |
0.4 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
50 |
HK-B1500/0.4 |
1500 |
0.4 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
50 |
HK-B1800/0.3 |
1800 |
0.3 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
53 |
HK-B2000/0.3 |
2000 |
0.3 |
750 |
380 |
RPP/SS304(可選) |
DN32 |
承插 socket/法蘭flange |
53 |
HK--C2400 | 2400 | 0.4 | 1100 | 380 | RPP/SS304(可選) | DN40 | 法蘭flange | 75 |
HK--C2800 | 2800 | 0.3 | 1500 | 380 | RPP/SS304(可選) | DN40 | 法蘭flange | 75 |
HK--C3300 | 3300 | 0.3 | 1500 | 380 | RPP/SS304(可選) | DN40 | 法蘭flange | 75 |
計量泵過流部分材質(zhì)表
HK系列機械隔膜計量泵過流部分材質(zhì)表 | |||||
零配件名稱 Part Name | 過流部分材質(zhì)over-current part | ||||
泵 頭 Pump head | RPP | PVC | PVDF | SUS304 | SUS316L |
隔 膜 Diaphragm | PTFE+橡膠(Rubber) | PTFE+橡膠(Rubber) | PTFE+橡膠(Rubber) | PTFE+橡膠(Rubber) | PTFE+橡膠(Rubber) |
單向閥 Check Valves | RPP | PVC | PVDF | SUS304 | SUS316L |
閥球座 Ball seat | PTFE/PP | PTFE/PVC | PTFE | SUS304 | SUS316L |
閥 球 Ball | 陶瓷 Ceramics | 陶瓷 Ceramics | 陶瓷 Ceramics | 陶瓷 Ceramics | 陶瓷 Ceramics |
O型密封圈 O-ring | 氟橡膠(FKM) | 氟橡膠(FKM) | PTFE | PTFE | PTFE |
569003100P伯格空濾外殼569000503P空濾壓差指示器1622515680博萊特進氣閥1625165331博萊特進氣閥2906904300阿特拉斯恒溫閥保養(yǎng)包02250138-090壽力空壓機配件02250145-097壽力空壓機配件02250155-971壽力空壓機配件02250155-970壽力空壓機配件C14200空氣過濾器外殼C16400空氣過濾器外殼C20500空氣過濾器外殼C23610空氣過濾器外殼C25860空氣過濾器外殼C25710空氣過濾器外殼C30810空氣過濾器外殼C281440空氣過濾器外殼C630空氣過濾器外殼C1140空氣過濾器外殼C1250空氣過濾器外殼4520092910空氣過濾器總成4520092920空氣過濾器總成4540092910空氣過濾器總成4550092910空氣過濾器總成4560092910空氣過濾器總成4486085951空氣過濾器總成4570092940空氣過濾器總成4580092940空氣過濾器總成4403077997空氣過濾器總成4403072998空氣過濾器總成4403072999空氣過濾器總成4404077997空氣過濾器總成4404077998空氣過濾器總成4404077999空氣過濾器總成4405077995空氣過濾器總成4405077996空氣過濾器總成4405077997空氣過濾器總成4406077998空氣過濾器總成4405077999空氣過濾器總成油氣分離器22051181462205117483空壓機濾芯1625165740空壓機濾芯1089057520阿特拉斯壓差傳感器64EB803登?諌簷C配件64EB805登?諌簷C配件TVS15170博萊特溫控閥TVSC80B博萊特溫控閥G40259200-010昆西空壓機配件24038846英格索蘭空壓機配件2104080019熱控制閥2104080020熱控制閥ATC35033英格索蘭空壓機配件ATC35033埃爾特空壓機配件2605702930復盛機油過濾器711823E1-301ETY369復盛機油過濾器阿斯埃爾離心機專用自潔式空濾芯離心機專用自潔式空濾芯3003497625離心機專用自潔式空濾芯3003003468離心機專用自潔式空濾芯3003001068離心機專用自潔式空濾芯3003000968離心機專用自潔式空濾芯3003001168離心機專用自潔式空濾芯離心機自潔式空濾芯空分專用自潔式空濾芯空分設備自潔式空濾芯2906096800阿特拉斯軸封維修包
23733306最小壓力閥23698715進氣閥23800584壓力傳感器19035641溫度傳感器23759236過濾網(wǎng)23699978空氣過濾器23711428機油過濾器23716475油氣分離器23702772電機潤滑脂100001110進氣閥維修包
Mouse Interleukin 15 (IL-15)ELISA KitCatalog No. CSB-E04604m(96T) This immunoassay kit allows for the in vitro quantitative determination of mouseIL-15 concentrations in serum, plasma and other biological fluids. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2INTRODUCTIONInterleukin 15 (IL-15) is a cytokine with structural similarity to IL-2 that issecreted by mononuclear phagocytes (and some other cells) followinginfection by virus(es). This cytokine induces cell proliferation of natural killercells; cells of the innate immune system whose principal role is to kill virallyinfected cells.The protein encoded by this gene is a cytokine that regulates T and naturalkiller cell activation and proliferation. This cytokine and interleukin 2 sharemany biological activities. They are found to bind common hematopoietinreceptor subunits, and may compete for the same receptor, and thusnegatively regulate each other's activity. The number of CD8+ memory cells isshown to be controlled by a balance between this cytokine and IL2. Thiscytokine induces the activation of JAK kinases, as well as the phosphorylationand activation of transcription activators STAT3, STAT5, and STAT6. Studiesof the mouse counterpart suggested that this cytokine may increase theexpression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through thetranscription activation activity of STAT6, and thus prevent apoptosis. Twoalternatively spliced transcript variants of this gene encoding the same proteinhave been reported.Maintenance of memory cells does not appear to require persistence of theoriginal antigen; instead, survival signals for memory lymphocytes areprovided by cytokines such as IL-15. In transgenic mice that have the IL-15receptor alpha (IL-15Ralpha) gene knocked out, natural killer cells cells do notdevelop.3PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated with an antibodyspecific to IL-15. Standards or samples are then added to the appropriatemicrotiter plate wells with a biotin-conjugated polyclonal antibody preparationspecific for IL-15 and Avidin conjugated to Horseradish Peroxidase (HRP) isadded to each microplate well and incubated. Then a TMB (3,3'5, 5'tetramethyl-benzidine) substrate solution is added to each well. Only thosewells that contain IL-15, biotin-conjugated antibody and enzyme-conjugatedAvidin will exhibit a change in color. The enzyme-substrate reaction isterminated by the addition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Theconcentration of IL-15 in the samples is then determined by comparing theO.D. of the samples to the standard curve.DETECTION RANGE6.25 pg/ml-400 pg/ml. The standard curve concentrations used for the ELISA’swere 400 pg/ml, 200 pg/ml,100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25pg/ml.SPECIFICITYThis assay recognizes recombinant and natural mouse IL-15. No significantcross-reactivity or interference was observed.SENSITIVITYThe minimum detectable dose of mouse IL-15 is typically less than 1.56 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as4the lowest protein concentration that could be differentiated from zero.MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard 2Sample Diluent 1 x 20 mlBiotin-antibody Diluent 1 x 10 mlHRP-avidin Diluent 1 x 10 mlBiotin-antibody 1 x 120μlHRP-avidin 1 x 120μlWash Buffer1 x 20 ml(25×concentrate)TMB Substrate 1 x 10 mlStop Solution 1 x 10 mlSTORAGE1. Unopened test kits should be stored at 2-8 ° C upon receipt and themicrotiter plate should be kept in a sealed bag. The test kit may be usedthroughout the expiration date of the kit, provided it is stored as prescribedabove. Refer to the package label for the expiration date.2. Opened test plate should be stored at 2-8°C in the aluminum foil bag withdesiccants to minimize exposure to damp air. The kits will remain stableuntil the expiring date shown, provided it is stored as prescribed above.53. A microtiter plate reader with a bandwidth of 10 nm or less and an opticaldensity range of 0-3 OD or greater at 450nm wavelength is acceptable foruse in absorbance measurement.REAGENT PREPARATIONBring all reagents to room temperature before use.1. Wash Buffer If crystals have formed in the concentrate, warm up toroom temperature and mix gently until the crystals have completelydissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized ordistilled water to prepare 500 ml of Wash Buffer.2. Standard Centrifuge the standard vial at 6000-10000rpm for 30s.Reconstitute the Standard with 1.0 ml of Sample Diluent. Thisreconstitution produces a stock solution of 400 pg/ml. Allow the standardto sit for a minimum of 15 minutes with gentle agitation prior to makingserial dilutions. The undiluted standard serves as the high standard (400pg/ml). The Sample Diluent serves as the zero standard (0 pg/ml).Prepare fresh for each assay. Use within 4 hours and discard after use.3. Biotin-antibody Centrifuge the vial before opening. Dilute to the workingconcentration using Biotin-antibody Diluent(1:100), respectively.4. HRP-avidin Centrifuge the vial before opening. Dilute to the workingconcentration using HRP-avidin Diluent(1:100), respectively.Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye,hand, face, and clothing protection when using this material.OTHER SUPPLIES REQUIRED Microplate reader capable of measuring absorbance at 450 nm, with thecorrection wavelength set at 540 nm or 570 nm. Pipettes and pipette tips.6 Deionized or distilled water. Squirt bottle, manifold dispenser, or automated microplate washer. An incubator which can provide stable incubation conditions up to37°C±0.5°C.SAMPLE COLLECTION AND STORAGE Serum Use a serum separator tube (SST) and allow samples to clot for30 minutes before centrifugation for 15 minutes at 1000 g. Remove serumand assay immediately or aliquot and store samples at -20°C. Centrifugethe sample again after thawing before the assay. Avoid repeatedfreeze-thaw cycles. Plasma Collect plasma using citrate, EDTA, or heparin as ananticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes ofcollection. Assay immediately or aliquot and store samples at -20°C.Centrifuge the sample again after thawing before the assay. Avoidrepeated freeze-thaw cycles.Note: Grossly hemolyzed samples are not suitable for use in this assay.ASSAY PROCEDUREBring all reagents and samples to room temperature before use. It is recommendedthat all samples, standards, and controls be assayed in duplicate. All the reagentsshould be added directly to the liquid level in the well. The pipette should avoidcontacting the inner wall of the well.1. Add 100μl of Standard, Blank, or Sample per well. Cover with theadhesive strip. Incubate for 2 hours at 37°C.72. Remove the liquid of each well, don’t wash.3. Add 100μl of Biotin-antibody working solution to each well. Incubate for 1hour at 37°C. Biotin-antibody working solution may appear cloudy. Warmup to room temperature and mix gently until solution appears uniform.4. Aspirate each well and wash, repeating the process three times for a totalof three washes. Wash: Fill each well with Wash Buffer (200μl) and let itstand for 2 minutes, then remove the liquid by flicking the plate over a sink.The remaining drops are removed by patting the plate on a paper towel.Complete removal of liquid at each step is essential to good performance.5. Add 100μl of HRP-avidin working solution to each well. Cover themicrotiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.6. Repeat the aspiration and wash three times as step 4.7. Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at37°C. Keeping the plate away from drafts and other temperaturefluctuations in the dark.8. Add 50μl of Stop Solution to each well when the first four wells containingthe highest concentration of standards develop obvious blue color. If colorchange does not appear uniform, gently tap the plate to ensure thoroughmixing.9. Determine the optical density of each well within 30 minutes, using amicroplate reader set to 450 nm.CALCULATION OF RESULTSUsing the professional soft "Curve Exert 1.3" to make a standard curve isrecommended, which can be downloaded from our web.Average the duplicate readings for each standard, control, and sample andsubtract the average zero standard optical density. Create a standard curve byreducing the data using computer software capable of generating a four8parameter logistic (4-PL) curve-fit. As an alternative, construct a standardcurve by plotting the mean absorbance for each standard on the y-axis againstthe concentration on the x-axis and draw a best fit curve through the points onthe graph. The data may be linearized by plotting the log of the IL-15concentrations versus the log of the O.D. and the best fit line can bedetermined by regression analysis. This procedure will produce an adequatebut less precise fit of the data. If samples have been diluted, the concentrationread from the standard curve must be multiplied by the dilution factor.LIMITATIONS OF THE PROCEDURE The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. It is important that the Standard Diluent selected for the standard curve beconsistent with the samples being assayed. If samples generate values higher than the highest standard, dilute thesamples with the appropriate Standard Diluent and repeat the assay. Any variation in Standard Diluent, operator, pipetting technique, washingtechnique, incubation time or temperature, and kit age can cause variationin binding. This assay is designed to eliminate interference by soluble receptors,binding proteins, and other factors present in biological samples. Until allfactors have been tested in the Immunoassay, the possibility ofinterference cannot be excluded.TECHNICAL HINTS Centrifuge vials before opening to collect contents. When mixing or reconstituting protein solutions, always avoid foaming. To avoid cross-contamination, change pipette tips between additions ofeach standard level, between sample additions, and between reagent9additions. Also, use separate reservoirs for each reagent. When using an automated plate washer, adding a 30 second soak periodfollowing the addition of wash buffer, and/or rotating the plate 180 degreesbetween wash steps may improve assay precision. To ensure accurate results, proper adhesion of plate sealers duringincubation steps is necessary. Substrate Solution should remain colorless or light blue until added to theplate. Keep Substrate Solution protected from light. Substrate Solutionshould change from colorless or light blue to gradations of blue. Stop Solution should be added to the plate in the same order as theSubstrate Solution. The color developed in the wells will turn from blue toyellow upon addition of the Stop Solution. Wells that are green in colorindicate that the Stop Solution has not mixed thoroughly with the SubstrateSolution.