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Catolog Number：LF0001

Features:

For DNA transfection to mammalian cells. 1. Simplest transfection protocol – ready to use, just mix with DNA ａnd vortex; 2. Superior transfection efficiency; 3. Effective for both adherent ａnd suspension cells; 4. Minimal cytotoxicity; 5. Change of cell culture media after transfection is recommended but not required; 6. Highly reproducible results; 7. Product stable at 4°C ａnd at -20°C.

Description:

Lipofectine is a proprietary cationic lipid-based transfection reagent for mammalian cells. The ratio of Lipofectine to plasmid DNA is fixed at 50 ul Lipofectine for 1 ug of plasmid DNA. Lipofectine is formulated in a ready-to-use format - all users need to do is add DNA ａnd vortex the mix. After a 15-minute incubation, the transfection mix can be added to cells. As judged by target protein production, the transfection efficiency of Lipofectine is consistently higher than that of similar product from other suppliers. Protocol for transfection of adherent cells: 1. For each microgram of DNA to be transfected, add 50 ul of Lipofectine; 2. Flick, rock, pipet up ａnd down, or vortex the mix for 5 seconds; 3. Incubate the mix at room temperature for 15 minutes; 4. Add the mix to media ａnd cells. Note: Change of media 12 to 24 hour post transfection may improve cell growth condition, ａnd is recommended. With Lipofectine, change of media is not absolutely required. Protocol for transfection of suspension cells: Lipofectine has proven to be an effective reagent to transfect suspension cells. The ideal cell density at time of transfection is 1 x 10⁶ per ml. Therefore, the day before transfection, suspension CHO cells are seeded at 0.5 x 10⁶ per ml in media such as ProCHO （Lonza） or CD-CHO （Invitrogen） without serum. The amount of DNA used in transfection should be 3 µg per ml of cells, which yields optimal cell growth ａnd target protein expression. Transfection mix can be prepared exactly as described in the previous section. The transfection mix is then added to the suspension cells. Media replacement is recommended 12 to 24 hour post transfection, ａnd data has shown that media replacement can increase cell growth rate after transfection for suspension. For protein or antibody production, cell media can be harvested 4-7 days post transfection. Note: Each cell line ａnd plasmid is different. The protocols ａnd recommendations are for CHO cells. Users should optimize the condition for their cell lines ａnd plasmids.

 

特點

Quintix 能夠極大地簡化在電子表格中處理數(shù)據(jù)的流程。 如何實現(xiàn)？將 Quintix 的 USB 端口連接到計算機之后，只需打開您所需的 Microsoft® Office 程序。Quintix 會以您選定的格式（文本或數(shù)字）提供數(shù)據(jù)，便于輕松而準確的電子表格計算。不需要使用其它軟件或特殊配置設(shè)置。

在嚴苛的“模擬測試室”內(nèi)，Quintix 的測試樣機經(jīng)過了 1,000,000次以上負載應(yīng)力測試，以確保在不同重量負載下，經(jīng)過多年的日常頻繁使用之后，天平仍能提供如第一天工作時一樣的準確結(jié)果。

溫度波動會對稱重結(jié)果的精確性產(chǎn)生影響。這就是每臺 Quintix均配備 isoCAL 內(nèi)部校準和調(diào)整功能的原因，此功能可確保稱重結(jié)果始終準確。每次運行 isoCAL 時，Quintix 內(nèi)的 Cal Audit Trail 會記錄該程序的所有數(shù)據(jù)，因而可以跟蹤，以用于質(zhì)量保證。

Quintix 能夠自動檢測sartorius 標(biāo)準打印機 YDP40。通過人 機對話式的用戶引導(dǎo)（例如 通過輸入正確的樣本 ID 編號），Quintix 能夠幫助打印機輸出無差錯的結(jié)果。

技術(shù)數(shù)據(jù)

應(yīng)用

配方－－允許您快速稱量配方中各個組分的重量，具有100% 可跟蹤性。

組分－－使您可以輕松地稱出配方中各組分的重量并放入獨立的容器之內(nèi)，并且隨時獲取總稱重量。

統(tǒng)計－－當(dāng)您需要知道整組不同樣品的標(biāo)準偏差和其它統(tǒng)計數(shù)據(jù)時，應(yīng)用該程序即可減輕工作量，使您無需將時間耗費在枯 燥的數(shù)據(jù)統(tǒng)計工作上。

轉(zhuǎn)換－－當(dāng)您需要使用系數(shù)來換算重量，例如計算每個單位 面積的重量時，天平自動進行數(shù)學(xué)計算。

密度－－無需將時間耗費在確定不規(guī)則形狀的實心物體的密度上。

檢重－－當(dāng)您需要快速確定每份樣品是否處于特定偏差范圍 之內(nèi)時，該應(yīng)用程序可幫助您快速處理一系列樣品。

稱重－－所有Quintix 天平均配有賽多利斯精心設(shè)計的 isoCAL － 溫度時間觸發(fā)的全自動內(nèi)部校準和調(diào)整功 能，以確保稱重結(jié)果完全準確。

峰值保持－－該功能被激活時，會鎖定 一個時間段內(nèi)的最大讀數(shù)；還可用于顯示屏被大體積樣品遮擋時鎖定讀數(shù)。

不穩(wěn)定稱重－－當(dāng)您需要在完全不穩(wěn)定的環(huán)境下稱重，或者想要測 量好動的小鼠的重量時， 該功能可提供穩(wěn)定的結(jié)果。

百分比－－在您需要確定樣本與參考標(biāo)準之間的百分比偏差時，能夠方便地對樣本進行比較。

計數(shù)－－在您需要了解一個包裝袋 內(nèi)有多少件完全相同的部件（例如藥片）時，為您提供確切的數(shù)量。